Counteracting the effect of leukemia exosomes by antiangiogenic gold nanoparticles

Catarina Roma-Rodrigues, Alexandra R Fernandes, Pedro V BaptistaUCIBIO, Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, Campus de Caparica, Caparica 2829-516, PortugalCorrespondence: Alexandra R Fernandes; Pedro V BaptistaUCIBIO,...

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Autores principales:	Roma-Rodrigues C, Fernandes AR, Baptista PV
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	Chronic myeloid leukemia exosomes chorioallantoic membrane gold nanoparticles angiogenesis Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/6c1e8356c1824deab5145fc9f5c1203a
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spelling	oai:doaj.org-article:6c1e8356c1824deab5145fc9f5c1203a2021-12-02T09:23:10ZCounteracting the effect of leukemia exosomes by antiangiogenic gold nanoparticles1178-2013https://doaj.org/article/6c1e8356c1824deab5145fc9f5c1203a2019-08-01T00:00:00Zhttps://www.dovepress.com/counteracting-the-effect-of-leukemia-exosomes-by-antiangiogenic-gold-n-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Catarina Roma-Rodrigues, Alexandra R Fernandes, Pedro V BaptistaUCIBIO, Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, Campus de Caparica, Caparica 2829-516, PortugalCorrespondence: Alexandra R Fernandes; Pedro V BaptistaUCIBIO, Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, Campus de Caparica, Caparica 2829-516, PortugalTel +351 21 294 8530Fax +351 21 294 8530Email ma.fernandes@fct.unl.pt pmvb@fct.unl.ptPurpose: Progression of chronic myeloid leukemia (CML) is frequently associated with increased angiogenesis at the bone marrow mediated by exosomes. The capability of gold nanoparticles (AuNPs) functionalized with antiangiogenic peptides to hinder the formation of new blood vessels has been demonstrated in a chorioallantoic membrane (CAM) model.Methods: Exosomes of K562 CML cell line were isolated and their angiogenic effect assessed in a CAM model. AuNPs functionalized with antiangiogenic peptides were used to block the angiogenic effect of CML-derived exosomes, assessed by evaluation of expression levels of key modulators involved in angiogenic pathways - VEGFA, VEGFR1 (also known as FLT1) and IL8.Results: Exosomes isolated from K562 cells promoted the doubling of newly formed vessels associated with the increase of VEGFR1 expression. This is a concentration and time-dependent effect. The AuNPs functionalized with antiangiogenic peptides were capable to block the angiogenic effect by modulating VEGFR1 associated pathway.Conclusion: Exosomes derived from blast cells are capable to trigger (neo)-angiogenesis, a key factor for the progression and spreading of cancer, in particular in CML. AuNPs functionalized with specific antiangiogenic peptides are capable to block the effect of the exosomes produced by malignant cells via modulation of the intrinsic VEGFR pathway. Together, these data highlight the potential of nanomedicine-based strategies against cancer proliferation.Keywords: chronic myeloid leukemia, exosomes, chorioallantoic membrane, gold nanoparticles, angiogenesisRoma-Rodrigues CFernandes ARBaptista PVDove Medical PressarticleChronic myeloid leukemiaexosomeschorioallantoic membranegold nanoparticlesangiogenesisMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 6843-6854 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Chronic myeloid leukemia exosomes chorioallantoic membrane gold nanoparticles angiogenesis Medicine (General) R5-920
spellingShingle	Chronic myeloid leukemia exosomes chorioallantoic membrane gold nanoparticles angiogenesis Medicine (General) R5-920 Roma-Rodrigues C Fernandes AR Baptista PV Counteracting the effect of leukemia exosomes by antiangiogenic gold nanoparticles
description	Catarina Roma-Rodrigues, Alexandra R Fernandes, Pedro V BaptistaUCIBIO, Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, Campus de Caparica, Caparica 2829-516, PortugalCorrespondence: Alexandra R Fernandes; Pedro V BaptistaUCIBIO, Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, Campus de Caparica, Caparica 2829-516, PortugalTel +351 21 294 8530Fax +351 21 294 8530Email ma.fernandes@fct.unl.pt pmvb@fct.unl.ptPurpose: Progression of chronic myeloid leukemia (CML) is frequently associated with increased angiogenesis at the bone marrow mediated by exosomes. The capability of gold nanoparticles (AuNPs) functionalized with antiangiogenic peptides to hinder the formation of new blood vessels has been demonstrated in a chorioallantoic membrane (CAM) model.Methods: Exosomes of K562 CML cell line were isolated and their angiogenic effect assessed in a CAM model. AuNPs functionalized with antiangiogenic peptides were used to block the angiogenic effect of CML-derived exosomes, assessed by evaluation of expression levels of key modulators involved in angiogenic pathways - VEGFA, VEGFR1 (also known as FLT1) and IL8.Results: Exosomes isolated from K562 cells promoted the doubling of newly formed vessels associated with the increase of VEGFR1 expression. This is a concentration and time-dependent effect. The AuNPs functionalized with antiangiogenic peptides were capable to block the angiogenic effect by modulating VEGFR1 associated pathway.Conclusion: Exosomes derived from blast cells are capable to trigger (neo)-angiogenesis, a key factor for the progression and spreading of cancer, in particular in CML. AuNPs functionalized with specific antiangiogenic peptides are capable to block the effect of the exosomes produced by malignant cells via modulation of the intrinsic VEGFR pathway. Together, these data highlight the potential of nanomedicine-based strategies against cancer proliferation.Keywords: chronic myeloid leukemia, exosomes, chorioallantoic membrane, gold nanoparticles, angiogenesis
format	article
author	Roma-Rodrigues C Fernandes AR Baptista PV
author_facet	Roma-Rodrigues C Fernandes AR Baptista PV
author_sort	Roma-Rodrigues C
title	Counteracting the effect of leukemia exosomes by antiangiogenic gold nanoparticles
title_short	Counteracting the effect of leukemia exosomes by antiangiogenic gold nanoparticles
title_full	Counteracting the effect of leukemia exosomes by antiangiogenic gold nanoparticles
title_fullStr	Counteracting the effect of leukemia exosomes by antiangiogenic gold nanoparticles
title_full_unstemmed	Counteracting the effect of leukemia exosomes by antiangiogenic gold nanoparticles
title_sort	counteracting the effect of leukemia exosomes by antiangiogenic gold nanoparticles
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/6c1e8356c1824deab5145fc9f5c1203a
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Counteracting the effect of leukemia exosomes by antiangiogenic gold nanoparticles

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