Extraction, Structural Characterization, and Anti-Hepatocellular Carcinoma Activity of Polysaccharides From Panax ginseng Meyer

Polysaccharides are the main active ingredients of ginseng. To extract the most effective polysaccharides against hepatocellular carcinoma (HCC), we isolated and characterized the polysaccharides from the mountain cultivated ginseng (MCG) and compared their composition and cytotoxic effect with cult...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Hui Jia, Bin Zhao, Fangfang Zhang, Ramesh Kumar Santhanam, Xinying Wang, Jincai Lu
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Panax ginseng Meyer MCG polysaccharides NMR HCC GC–MS analysis Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/6c2151440aaa4a7b8ea00815c993718f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:6c2151440aaa4a7b8ea00815c993718f
record_format	dspace
spelling	oai:doaj.org-article:6c2151440aaa4a7b8ea00815c993718f2021-12-01T12:38:29ZExtraction, Structural Characterization, and Anti-Hepatocellular Carcinoma Activity of Polysaccharides From Panax ginseng Meyer2234-943X10.3389/fonc.2021.785455https://doaj.org/article/6c2151440aaa4a7b8ea00815c993718f2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fonc.2021.785455/fullhttps://doaj.org/toc/2234-943XPolysaccharides are the main active ingredients of ginseng. To extract the most effective polysaccharides against hepatocellular carcinoma (HCC), we isolated and characterized the polysaccharides from the mountain cultivated ginseng (MCG) and compared their composition and cytotoxic effect with cultivated ginseng (CG) polysaccharide against HepG2 cell lines for the first time. MCG polysaccharides and CG polysaccharides were fractionated into two fractions such as MTPS-1, MTPS-2 and CTPS-1, CTPS-2 by salting out, respectively. Compared to CG, MCG possessed appreciable cytotoxic effect against HepG2 cells among that MTPS-1 possess fortified effect. Then, MTPS-1 was selected for further isolation process and seven acidic polysaccharides (MCGP-1–MCGP-7) were obtained using ethanol precipitation, ion-exchange, and gel permeation chromatography techniques. Structural characteristics of the polysaccharides (MCGP-1–MCGP-7) were done by adapting methylation/GC-MS and NMR analysis. Overall, MCGP-3 polysaccharide was found to possess significant cytotoxic effect against HepG2 cells with the IC50 value.Hui JiaBin ZhaoFangfang ZhangRamesh Kumar SanthanamXinying WangJincai LuJincai LuFrontiers Media S.A.articlePanax ginseng MeyerMCG polysaccharidesNMRHCCGC–MS analysisNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENFrontiers in Oncology, Vol 11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Panax ginseng Meyer MCG polysaccharides NMR HCC GC–MS analysis Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	Panax ginseng Meyer MCG polysaccharides NMR HCC GC–MS analysis Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Hui Jia Bin Zhao Fangfang Zhang Ramesh Kumar Santhanam Xinying Wang Jincai Lu Jincai Lu Extraction, Structural Characterization, and Anti-Hepatocellular Carcinoma Activity of Polysaccharides From Panax ginseng Meyer
description	Polysaccharides are the main active ingredients of ginseng. To extract the most effective polysaccharides against hepatocellular carcinoma (HCC), we isolated and characterized the polysaccharides from the mountain cultivated ginseng (MCG) and compared their composition and cytotoxic effect with cultivated ginseng (CG) polysaccharide against HepG2 cell lines for the first time. MCG polysaccharides and CG polysaccharides were fractionated into two fractions such as MTPS-1, MTPS-2 and CTPS-1, CTPS-2 by salting out, respectively. Compared to CG, MCG possessed appreciable cytotoxic effect against HepG2 cells among that MTPS-1 possess fortified effect. Then, MTPS-1 was selected for further isolation process and seven acidic polysaccharides (MCGP-1–MCGP-7) were obtained using ethanol precipitation, ion-exchange, and gel permeation chromatography techniques. Structural characteristics of the polysaccharides (MCGP-1–MCGP-7) were done by adapting methylation/GC-MS and NMR analysis. Overall, MCGP-3 polysaccharide was found to possess significant cytotoxic effect against HepG2 cells with the IC50 value.
format	article
author	Hui Jia Bin Zhao Fangfang Zhang Ramesh Kumar Santhanam Xinying Wang Jincai Lu Jincai Lu
author_facet	Hui Jia Bin Zhao Fangfang Zhang Ramesh Kumar Santhanam Xinying Wang Jincai Lu Jincai Lu
author_sort	Hui Jia
title	Extraction, Structural Characterization, and Anti-Hepatocellular Carcinoma Activity of Polysaccharides From Panax ginseng Meyer
title_short	Extraction, Structural Characterization, and Anti-Hepatocellular Carcinoma Activity of Polysaccharides From Panax ginseng Meyer
title_full	Extraction, Structural Characterization, and Anti-Hepatocellular Carcinoma Activity of Polysaccharides From Panax ginseng Meyer
title_fullStr	Extraction, Structural Characterization, and Anti-Hepatocellular Carcinoma Activity of Polysaccharides From Panax ginseng Meyer
title_full_unstemmed	Extraction, Structural Characterization, and Anti-Hepatocellular Carcinoma Activity of Polysaccharides From Panax ginseng Meyer
title_sort	extraction, structural characterization, and anti-hepatocellular carcinoma activity of polysaccharides from panax ginseng meyer
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/6c2151440aaa4a7b8ea00815c993718f
work_keys_str_mv	AT huijia extractionstructuralcharacterizationandantihepatocellularcarcinomaactivityofpolysaccharidesfrompanaxginsengmeyer AT binzhao extractionstructuralcharacterizationandantihepatocellularcarcinomaactivityofpolysaccharidesfrompanaxginsengmeyer AT fangfangzhang extractionstructuralcharacterizationandantihepatocellularcarcinomaactivityofpolysaccharidesfrompanaxginsengmeyer AT rameshkumarsanthanam extractionstructuralcharacterizationandantihepatocellularcarcinomaactivityofpolysaccharidesfrompanaxginsengmeyer AT xinyingwang extractionstructuralcharacterizationandantihepatocellularcarcinomaactivityofpolysaccharidesfrompanaxginsengmeyer AT jincailu extractionstructuralcharacterizationandantihepatocellularcarcinomaactivityofpolysaccharidesfrompanaxginsengmeyer AT jincailu extractionstructuralcharacterizationandantihepatocellularcarcinomaactivityofpolysaccharidesfrompanaxginsengmeyer
_version_	1718405178225328128

Extraction, Structural Characterization, and Anti-Hepatocellular Carcinoma Activity of Polysaccharides From Panax ginseng Meyer

Ejemplares similares