Genome-wide identification and analysis of long non-coding RNAs involved in fatty acid biosynthesis in young soybean pods

Abstract Long non-coding RNAs (lncRNAs) are non-coding RNAs of more than 200 nucleotides. To date, the roles of lncRNAs in soybean fatty acid synthesis have not been fully studied. Here, the low-linolenic acid mutant ‘MT72′ and the wild-type control ‘JN18′ were used as materials. The lncRNAs in youn...

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Autores principales:	Bohan Ma, Aijing Zhang, Qiuzhu Zhao, Zeyuan Li, Abraham Lamboro, Haobo He, Yue Li, Suqi Jiao, Shuyan Guan, Siyan Liu, Dan Yao, Jun Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6c227e0862834708878c6de9ab711793
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spelling	oai:doaj.org-article:6c227e0862834708878c6de9ab7117932021-12-02T14:37:46ZGenome-wide identification and analysis of long non-coding RNAs involved in fatty acid biosynthesis in young soybean pods10.1038/s41598-021-87048-72045-2322https://doaj.org/article/6c227e0862834708878c6de9ab7117932021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-87048-7https://doaj.org/toc/2045-2322Abstract Long non-coding RNAs (lncRNAs) are non-coding RNAs of more than 200 nucleotides. To date, the roles of lncRNAs in soybean fatty acid synthesis have not been fully studied. Here, the low-linolenic acid mutant ‘MT72′ and the wild-type control ‘JN18′ were used as materials. The lncRNAs in young pods at 30 and 40 days (d) after flowering were systematically identified and analyzed using transcriptome sequencing technology combined with bioinformatics tools. A total of 39,324 lncRNAs and 561 differentially expressed lncRNAs were identified. A lncRNAs-miRNAs-protein-coding genes (mRNAs) network was constructed, and 46 lncRNAs, 46 miRNAs and 137 mRNAs were found to be correlated. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of 12 targeted mRNAs in the competing endogenous RNA network showed that these lncRNAs may be involved in the biological processes of fatty acid transport, lipid synthesis and cell division. Finally, the expression levels of differentially expressed lncRNAs, miRNAs and mRNAs were verified using qRT-PCR. The expression patterns of most genes were consistent with the sequencing results. In conclusion, new information was provided for the study of fatty acid synthesis by lncRNAs in young soybean pods.Bohan MaAijing ZhangQiuzhu ZhaoZeyuan LiAbraham LamboroHaobo HeYue LiSuqi JiaoShuyan GuanSiyan LiuDan YaoJun ZhangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-17 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Bohan Ma Aijing Zhang Qiuzhu Zhao Zeyuan Li Abraham Lamboro Haobo He Yue Li Suqi Jiao Shuyan Guan Siyan Liu Dan Yao Jun Zhang Genome-wide identification and analysis of long non-coding RNAs involved in fatty acid biosynthesis in young soybean pods
description	Abstract Long non-coding RNAs (lncRNAs) are non-coding RNAs of more than 200 nucleotides. To date, the roles of lncRNAs in soybean fatty acid synthesis have not been fully studied. Here, the low-linolenic acid mutant ‘MT72′ and the wild-type control ‘JN18′ were used as materials. The lncRNAs in young pods at 30 and 40 days (d) after flowering were systematically identified and analyzed using transcriptome sequencing technology combined with bioinformatics tools. A total of 39,324 lncRNAs and 561 differentially expressed lncRNAs were identified. A lncRNAs-miRNAs-protein-coding genes (mRNAs) network was constructed, and 46 lncRNAs, 46 miRNAs and 137 mRNAs were found to be correlated. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of 12 targeted mRNAs in the competing endogenous RNA network showed that these lncRNAs may be involved in the biological processes of fatty acid transport, lipid synthesis and cell division. Finally, the expression levels of differentially expressed lncRNAs, miRNAs and mRNAs were verified using qRT-PCR. The expression patterns of most genes were consistent with the sequencing results. In conclusion, new information was provided for the study of fatty acid synthesis by lncRNAs in young soybean pods.
format	article
author	Bohan Ma Aijing Zhang Qiuzhu Zhao Zeyuan Li Abraham Lamboro Haobo He Yue Li Suqi Jiao Shuyan Guan Siyan Liu Dan Yao Jun Zhang
author_facet	Bohan Ma Aijing Zhang Qiuzhu Zhao Zeyuan Li Abraham Lamboro Haobo He Yue Li Suqi Jiao Shuyan Guan Siyan Liu Dan Yao Jun Zhang
author_sort	Bohan Ma
title	Genome-wide identification and analysis of long non-coding RNAs involved in fatty acid biosynthesis in young soybean pods
title_short	Genome-wide identification and analysis of long non-coding RNAs involved in fatty acid biosynthesis in young soybean pods
title_full	Genome-wide identification and analysis of long non-coding RNAs involved in fatty acid biosynthesis in young soybean pods
title_fullStr	Genome-wide identification and analysis of long non-coding RNAs involved in fatty acid biosynthesis in young soybean pods
title_full_unstemmed	Genome-wide identification and analysis of long non-coding RNAs involved in fatty acid biosynthesis in young soybean pods
title_sort	genome-wide identification and analysis of long non-coding rnas involved in fatty acid biosynthesis in young soybean pods
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/6c227e0862834708878c6de9ab711793
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Genome-wide identification and analysis of long non-coding RNAs involved in fatty acid biosynthesis in young soybean pods

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