Determination of the composition of heterogeneous binder solutions by surface plasmon resonance biosensing

Abstract Surface plasmon resonance-based biosensors have been extensively applied to the characterization of the binding kinetics between purified (bio)molecules, thanks to robust data analysis techniques. However, data analysis for solutions containing multiple interactants is still at its infancy....

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Autores principales:	Jimmy Gaudreault, Benoît Liberelle, Yves Durocher, Olivier Henry, Gregory De Crescenzo
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6c26880f2fa54665b310f249ed96291e
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spelling	oai:doaj.org-article:6c26880f2fa54665b310f249ed96291e2021-12-02T14:11:30ZDetermination of the composition of heterogeneous binder solutions by surface plasmon resonance biosensing10.1038/s41598-021-83268-z2045-2322https://doaj.org/article/6c26880f2fa54665b310f249ed96291e2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83268-zhttps://doaj.org/toc/2045-2322Abstract Surface plasmon resonance-based biosensors have been extensively applied to the characterization of the binding kinetics between purified (bio)molecules, thanks to robust data analysis techniques. However, data analysis for solutions containing multiple interactants is still at its infancy. We here present two algorithms for (1) the reliable and accurate determination of the kinetic parameters of N interactants present at different ratios in N mixtures and (2) the estimation of the ratios of each interactant in a given mixture, assuming that their kinetic parameters are known. Both algorithms assume that the interactants compete to bind to an immobilized ligand in a 1:1 fashion and necessitate prior knowledge of the total concentration of all interactants combined. The effectiveness of these two algorithms was experimentally validated with a model system corresponding to mixtures of four small molecular weight drugs binding to an immobilized protein. This approach enables the in-depth characterization of mixtures using SPR, which may be of considerable interest for many drug discovery or development applications, notably for protein glycovariant analysis.Jimmy GaudreaultBenoît LiberelleYves DurocherOlivier HenryGregory De CrescenzoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jimmy Gaudreault Benoît Liberelle Yves Durocher Olivier Henry Gregory De Crescenzo Determination of the composition of heterogeneous binder solutions by surface plasmon resonance biosensing
description	Abstract Surface plasmon resonance-based biosensors have been extensively applied to the characterization of the binding kinetics between purified (bio)molecules, thanks to robust data analysis techniques. However, data analysis for solutions containing multiple interactants is still at its infancy. We here present two algorithms for (1) the reliable and accurate determination of the kinetic parameters of N interactants present at different ratios in N mixtures and (2) the estimation of the ratios of each interactant in a given mixture, assuming that their kinetic parameters are known. Both algorithms assume that the interactants compete to bind to an immobilized ligand in a 1:1 fashion and necessitate prior knowledge of the total concentration of all interactants combined. The effectiveness of these two algorithms was experimentally validated with a model system corresponding to mixtures of four small molecular weight drugs binding to an immobilized protein. This approach enables the in-depth characterization of mixtures using SPR, which may be of considerable interest for many drug discovery or development applications, notably for protein glycovariant analysis.
format	article
author	Jimmy Gaudreault Benoît Liberelle Yves Durocher Olivier Henry Gregory De Crescenzo
author_facet	Jimmy Gaudreault Benoît Liberelle Yves Durocher Olivier Henry Gregory De Crescenzo
author_sort	Jimmy Gaudreault
title	Determination of the composition of heterogeneous binder solutions by surface plasmon resonance biosensing
title_short	Determination of the composition of heterogeneous binder solutions by surface plasmon resonance biosensing
title_full	Determination of the composition of heterogeneous binder solutions by surface plasmon resonance biosensing
title_fullStr	Determination of the composition of heterogeneous binder solutions by surface plasmon resonance biosensing
title_full_unstemmed	Determination of the composition of heterogeneous binder solutions by surface plasmon resonance biosensing
title_sort	determination of the composition of heterogeneous binder solutions by surface plasmon resonance biosensing
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/6c26880f2fa54665b310f249ed96291e
work_keys_str_mv	AT jimmygaudreault determinationofthecompositionofheterogeneousbindersolutionsbysurfaceplasmonresonancebiosensing AT benoitliberelle determinationofthecompositionofheterogeneousbindersolutionsbysurfaceplasmonresonancebiosensing AT yvesdurocher determinationofthecompositionofheterogeneousbindersolutionsbysurfaceplasmonresonancebiosensing AT olivierhenry determinationofthecompositionofheterogeneousbindersolutionsbysurfaceplasmonresonancebiosensing AT gregorydecrescenzo determinationofthecompositionofheterogeneousbindersolutionsbysurfaceplasmonresonancebiosensing
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Determination of the composition of heterogeneous binder solutions by surface plasmon resonance biosensing

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