Docking protein 1 and free fatty acids are associated with insulin resistance in patients with type 2 diabetes mellitus

Objective Insulin resistance (IR) is a key defect in type 2 diabetes mellitus (T2DM); therefore, effective means of ameliorating IR are sought. Methods We performed a retrospective cohort study of 154 patients with T2DM and 39 with pre-diabetes (pre-DM). The effects of IR and a high concentration of...

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Autores principales:	Xiaoqin Ha, Xiaoling Cai, Huizhe Cao, Jie Li, Bo Yang, Ruru Jiang, Xin Li, Bin Li, Yuan Xin
Formato:	article
Lenguaje:	EN
Publicado:	SAGE Publishing 2021
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/6c2838e6bf7244e5946ed622c4a7dff4
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spelling	oai:doaj.org-article:6c2838e6bf7244e5946ed622c4a7dff42021-11-04T00:03:37ZDocking protein 1 and free fatty acids are associated with insulin resistance in patients with type 2 diabetes mellitus1473-230010.1177/03000605211048293https://doaj.org/article/6c2838e6bf7244e5946ed622c4a7dff42021-11-01T00:00:00Zhttps://doi.org/10.1177/03000605211048293https://doaj.org/toc/1473-2300Objective Insulin resistance (IR) is a key defect in type 2 diabetes mellitus (T2DM); therefore, effective means of ameliorating IR are sought. Methods We performed a retrospective cohort study of 154 patients with T2DM and 39 with pre-diabetes (pre-DM). The effects of IR and a high concentration of FFA on gene expression were determined using microarray analysis and quantitative reverse transcription polymerase chain reaction (RT-qPCR) in patients with T2DM or pre-DM. Results Serum FFA concentration and homeostasis model assessment of IR (HOMA-IR) were significantly higher in patients with T2DM but no obesity and in those with pre-DM than in controls. HOMA-IR was significantly associated with T2DM. RT-qPCR showed that the expression of FBJ murine osteosarcoma viral oncogene homolog ( FOS ) and AE binding protein 1 ( AEBP1 ) was much lower in the circulation of participants with obesity and diabetes. RT-qPCR showed that the expression of docking protein 1 ( DOK1 ) was significantly lower in the blood of participants with diabetes but no obesity and in those with pre-DM than in controls. Conclusions FFA and DOK1 are associated with IR in patients with T2DM but no obesity or pre-DM. The downregulation of DOK1 might inhibit lipid synthesis and induce lipolysis, inducing or worsening IR.Xiaoqin HaXiaoling CaiHuizhe CaoJie LiBo YangRuru JiangXin LiBin LiYuan XinSAGE PublishingarticleMedicine (General)R5-920ENJournal of International Medical Research, Vol 49 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Xiaoqin Ha Xiaoling Cai Huizhe Cao Jie Li Bo Yang Ruru Jiang Xin Li Bin Li Yuan Xin Docking protein 1 and free fatty acids are associated with insulin resistance in patients with type 2 diabetes mellitus
description	Objective Insulin resistance (IR) is a key defect in type 2 diabetes mellitus (T2DM); therefore, effective means of ameliorating IR are sought. Methods We performed a retrospective cohort study of 154 patients with T2DM and 39 with pre-diabetes (pre-DM). The effects of IR and a high concentration of FFA on gene expression were determined using microarray analysis and quantitative reverse transcription polymerase chain reaction (RT-qPCR) in patients with T2DM or pre-DM. Results Serum FFA concentration and homeostasis model assessment of IR (HOMA-IR) were significantly higher in patients with T2DM but no obesity and in those with pre-DM than in controls. HOMA-IR was significantly associated with T2DM. RT-qPCR showed that the expression of FBJ murine osteosarcoma viral oncogene homolog ( FOS ) and AE binding protein 1 ( AEBP1 ) was much lower in the circulation of participants with obesity and diabetes. RT-qPCR showed that the expression of docking protein 1 ( DOK1 ) was significantly lower in the blood of participants with diabetes but no obesity and in those with pre-DM than in controls. Conclusions FFA and DOK1 are associated with IR in patients with T2DM but no obesity or pre-DM. The downregulation of DOK1 might inhibit lipid synthesis and induce lipolysis, inducing or worsening IR.
format	article
author	Xiaoqin Ha Xiaoling Cai Huizhe Cao Jie Li Bo Yang Ruru Jiang Xin Li Bin Li Yuan Xin
author_facet	Xiaoqin Ha Xiaoling Cai Huizhe Cao Jie Li Bo Yang Ruru Jiang Xin Li Bin Li Yuan Xin
author_sort	Xiaoqin Ha
title	Docking protein 1 and free fatty acids are associated with insulin resistance in patients with type 2 diabetes mellitus
title_short	Docking protein 1 and free fatty acids are associated with insulin resistance in patients with type 2 diabetes mellitus
title_full	Docking protein 1 and free fatty acids are associated with insulin resistance in patients with type 2 diabetes mellitus
title_fullStr	Docking protein 1 and free fatty acids are associated with insulin resistance in patients with type 2 diabetes mellitus
title_full_unstemmed	Docking protein 1 and free fatty acids are associated with insulin resistance in patients with type 2 diabetes mellitus
title_sort	docking protein 1 and free fatty acids are associated with insulin resistance in patients with type 2 diabetes mellitus
publisher	SAGE Publishing
publishDate	2021
url	https://doaj.org/article/6c2838e6bf7244e5946ed622c4a7dff4
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Docking protein 1 and free fatty acids are associated with insulin resistance in patients with type 2 diabetes mellitus

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