Genome-Wide Identification and Expression Analysis of Terpene Synthase Genes in Cymbidium faberi
Terpene synthases (TPSs) are essential for forming terpenes, which play numerous functional roles in attracting pollinators, defending plants, and moderating the interaction between plants. TPSs have been reported in some orchids, but genome-wide identification of terpenes in Cymbidium faberi is sti...
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Frontiers Media S.A.
2021
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oai:doaj.org-article:6c283a0a3f054429aee47db9b87e32592021-12-01T01:33:15ZGenome-Wide Identification and Expression Analysis of Terpene Synthase Genes in Cymbidium faberi1664-462X10.3389/fpls.2021.751853https://doaj.org/article/6c283a0a3f054429aee47db9b87e32592021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.751853/fullhttps://doaj.org/toc/1664-462XTerpene synthases (TPSs) are essential for forming terpenes, which play numerous functional roles in attracting pollinators, defending plants, and moderating the interaction between plants. TPSs have been reported in some orchids, but genome-wide identification of terpenes in Cymbidium faberi is still lacking. In this study, 32 putative TPS genes were classified in C. faberi and divided into three subfamilies (TPS-a, TPS-b, and TPS-e/f). Motif and gene structure analysis revealed that most CfTPS genes had the conserved aspartate-rich DDxxD motif. TPS genes in the TPS-a and TPS-b subfamilies had variations in the RRX8W motif. Most cis-elements of CfTPS genes were found in the phytohormone responsiveness category, and MYC contained most of the numbers associated with MeJA responsiveness. The Ka/Ks ratios of 12/13 CfTPS gene pairs were less than one, indicated that most CfTPS genes have undergone negative selection. The tissue-specific expression patterns showed that 28 genes were expressed in at least one tissue in C. faberi, and TPS genes were most highly expressed in flowers, followed by leaves and pseudobulbs. In addition, four CfTPS genes were selected for the real-time reverse transcription quantitative PCR (RT-qPCR) experiment. The results revealed that CfTPS12, CfTPS18, CfTPS23, and CfTPS28 were mainly expressed in the full flowering stage. CfTPS18 could convert GPP to β-myrcene, geraniol, and α-pinene in vitro. These findings of CfTPS genes of C. faberi may provide valuable information for further studies on TPSs in orchids.Qian-Qian WangMeng-Jia ZhuXia YuYuan-Yang BiZhuang ZhouZhuang ZhouMing-Kun ChenJiating ChenDiyang ZhangYe AiZhong-Jian LiuZhong-Jian LiuZhong-Jian LiuSiren LanSiren LanFrontiers Media S.A.articleterpenesterpene synthaseorchidsfloral scentexpression analysisCymbidiumPlant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021) |
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| collection |
DOAJ |
| language |
EN |
| topic |
terpenes terpene synthase orchids floral scent expression analysis Cymbidium Plant culture SB1-1110 |
| spellingShingle |
terpenes terpene synthase orchids floral scent expression analysis Cymbidium Plant culture SB1-1110 Qian-Qian Wang Meng-Jia Zhu Xia Yu Yuan-Yang Bi Zhuang Zhou Zhuang Zhou Ming-Kun Chen Jiating Chen Diyang Zhang Ye Ai Zhong-Jian Liu Zhong-Jian Liu Zhong-Jian Liu Siren Lan Siren Lan Genome-Wide Identification and Expression Analysis of Terpene Synthase Genes in Cymbidium faberi |
| description |
Terpene synthases (TPSs) are essential for forming terpenes, which play numerous functional roles in attracting pollinators, defending plants, and moderating the interaction between plants. TPSs have been reported in some orchids, but genome-wide identification of terpenes in Cymbidium faberi is still lacking. In this study, 32 putative TPS genes were classified in C. faberi and divided into three subfamilies (TPS-a, TPS-b, and TPS-e/f). Motif and gene structure analysis revealed that most CfTPS genes had the conserved aspartate-rich DDxxD motif. TPS genes in the TPS-a and TPS-b subfamilies had variations in the RRX8W motif. Most cis-elements of CfTPS genes were found in the phytohormone responsiveness category, and MYC contained most of the numbers associated with MeJA responsiveness. The Ka/Ks ratios of 12/13 CfTPS gene pairs were less than one, indicated that most CfTPS genes have undergone negative selection. The tissue-specific expression patterns showed that 28 genes were expressed in at least one tissue in C. faberi, and TPS genes were most highly expressed in flowers, followed by leaves and pseudobulbs. In addition, four CfTPS genes were selected for the real-time reverse transcription quantitative PCR (RT-qPCR) experiment. The results revealed that CfTPS12, CfTPS18, CfTPS23, and CfTPS28 were mainly expressed in the full flowering stage. CfTPS18 could convert GPP to β-myrcene, geraniol, and α-pinene in vitro. These findings of CfTPS genes of C. faberi may provide valuable information for further studies on TPSs in orchids. |
| format |
article |
| author |
Qian-Qian Wang Meng-Jia Zhu Xia Yu Yuan-Yang Bi Zhuang Zhou Zhuang Zhou Ming-Kun Chen Jiating Chen Diyang Zhang Ye Ai Zhong-Jian Liu Zhong-Jian Liu Zhong-Jian Liu Siren Lan Siren Lan |
| author_facet |
Qian-Qian Wang Meng-Jia Zhu Xia Yu Yuan-Yang Bi Zhuang Zhou Zhuang Zhou Ming-Kun Chen Jiating Chen Diyang Zhang Ye Ai Zhong-Jian Liu Zhong-Jian Liu Zhong-Jian Liu Siren Lan Siren Lan |
| author_sort |
Qian-Qian Wang |
| title |
Genome-Wide Identification and Expression Analysis of Terpene Synthase Genes in Cymbidium faberi |
| title_short |
Genome-Wide Identification and Expression Analysis of Terpene Synthase Genes in Cymbidium faberi |
| title_full |
Genome-Wide Identification and Expression Analysis of Terpene Synthase Genes in Cymbidium faberi |
| title_fullStr |
Genome-Wide Identification and Expression Analysis of Terpene Synthase Genes in Cymbidium faberi |
| title_full_unstemmed |
Genome-Wide Identification and Expression Analysis of Terpene Synthase Genes in Cymbidium faberi |
| title_sort |
genome-wide identification and expression analysis of terpene synthase genes in cymbidium faberi |
| publisher |
Frontiers Media S.A. |
| publishDate |
2021 |
| url |
https://doaj.org/article/6c283a0a3f054429aee47db9b87e3259 |
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