Incorporation of heparin-binding proteins into preformed dextran sulfate-chitosan nanoparticles

Paula Zaman,1 Julia Wang,1 Adam Blau,1 Weiping Wang,2 Tina Li,1 Daniel S Kohane,2 Joseph Loscalzo,1 Ying-Yi Zhang1 1Department of Medicine, Brigham and Women’s Hospital, 2Department of Anesthesiology, Boston Children’s Hospital, Harvard Medical School, Boston, MA, USA Abstract:...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Zaman P, Wang J, Blau A, Wang W, Li T, Kohane DS, Loscalzo J, Zhang YY
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	polyelectrolyte complex polysaccharide glycan glycosaminoglycan Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/6c2acd9bf99141c4beee071a1351dfbc
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:6c2acd9bf99141c4beee071a1351dfbc
record_format	dspace
spelling	oai:doaj.org-article:6c2acd9bf99141c4beee071a1351dfbc2021-12-02T03:54:52ZIncorporation of heparin-binding proteins into preformed dextran sulfate-chitosan nanoparticles1178-2013https://doaj.org/article/6c2acd9bf99141c4beee071a1351dfbc2016-11-01T00:00:00Zhttps://www.dovepress.com/incorporation-of-heparin-binding-proteins-into-preformed-dextran-sulf-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Paula Zaman,1 Julia Wang,1 Adam Blau,1 Weiping Wang,2 Tina Li,1 Daniel S Kohane,2 Joseph Loscalzo,1 Ying-Yi Zhang1 1Department of Medicine, Brigham and Women’s Hospital, 2Department of Anesthesiology, Boston Children’s Hospital, Harvard Medical School, Boston, MA, USA Abstract: Incorporation of proteins into dextran sulfate (DS)-chitosan (CS) nanoparticles (DSCS NPs) is commonly performed using entrapment procedures, in which protein molecules are mixed with DS and CS until particle formation occurs. As DS is an analog of heparin, the authors examined whether proteins could be directly incorporated into preformed DSCS NPs through a heparin binding domain-mediated interaction. The authors formulated negatively-charged DSCS NPs, and quantified the amount of charged DS in the outer shell of the particles. The authors then mixed the DSCS NPs with heparin-binding proteins (SDF-1α, VEGF, FGF-2, BMP-2, or lysozyme) to achieve incorporation. Data show that for DSCS NPs containing 100 nmol charged glucose sulfate units in DS, up to ~1.5 nmol of monomeric or ~0.75 nmol of dimeric heparin-binding proteins were incorporated without significantly altering the size or zeta potential of the particles. Incorporation efficiencies of these proteins were 95%–100%. In contrast, serum albumin or serum globulin showed minimal incorporation (8% and 4%, respectively) in 50% physiological saline, despite their large adsorption in water (80% and 92%, respectively). The NP-incorporated SDF-1α and VEGF exhibited full activity and sustained thermal stability. An in vivo aerosolization study showed that NP-incorporated SDF-1α persisted in rat lungs for 72 h (~34% remaining), while free SDF-1α was no longer detectable after 16 h. As many growth factors and cytokines contain heparin-binding sites/domains, incorporation into preformed DSCS NPs could facilitate in vivo applications of these proteins. Keywords: polyelectrolyte complex, polysaccharide, glycan, glycosaminoglycanZaman PWang JBlau AWang WLi TKohane DSLoscalzo JZhang YYDove Medical Pressarticlepolyelectrolyte complexpolysaccharideglycanglycosaminoglycanMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 11, Pp 6149-6159 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	polyelectrolyte complex polysaccharide glycan glycosaminoglycan Medicine (General) R5-920
spellingShingle	polyelectrolyte complex polysaccharide glycan glycosaminoglycan Medicine (General) R5-920 Zaman P Wang J Blau A Wang W Li T Kohane DS Loscalzo J Zhang YY Incorporation of heparin-binding proteins into preformed dextran sulfate-chitosan nanoparticles
description	Paula Zaman,1 Julia Wang,1 Adam Blau,1 Weiping Wang,2 Tina Li,1 Daniel S Kohane,2 Joseph Loscalzo,1 Ying-Yi Zhang1 1Department of Medicine, Brigham and Women’s Hospital, 2Department of Anesthesiology, Boston Children’s Hospital, Harvard Medical School, Boston, MA, USA Abstract: Incorporation of proteins into dextran sulfate (DS)-chitosan (CS) nanoparticles (DSCS NPs) is commonly performed using entrapment procedures, in which protein molecules are mixed with DS and CS until particle formation occurs. As DS is an analog of heparin, the authors examined whether proteins could be directly incorporated into preformed DSCS NPs through a heparin binding domain-mediated interaction. The authors formulated negatively-charged DSCS NPs, and quantified the amount of charged DS in the outer shell of the particles. The authors then mixed the DSCS NPs with heparin-binding proteins (SDF-1α, VEGF, FGF-2, BMP-2, or lysozyme) to achieve incorporation. Data show that for DSCS NPs containing 100 nmol charged glucose sulfate units in DS, up to ~1.5 nmol of monomeric or ~0.75 nmol of dimeric heparin-binding proteins were incorporated without significantly altering the size or zeta potential of the particles. Incorporation efficiencies of these proteins were 95%–100%. In contrast, serum albumin or serum globulin showed minimal incorporation (8% and 4%, respectively) in 50% physiological saline, despite their large adsorption in water (80% and 92%, respectively). The NP-incorporated SDF-1α and VEGF exhibited full activity and sustained thermal stability. An in vivo aerosolization study showed that NP-incorporated SDF-1α persisted in rat lungs for 72 h (~34% remaining), while free SDF-1α was no longer detectable after 16 h. As many growth factors and cytokines contain heparin-binding sites/domains, incorporation into preformed DSCS NPs could facilitate in vivo applications of these proteins. Keywords: polyelectrolyte complex, polysaccharide, glycan, glycosaminoglycan
format	article
author	Zaman P Wang J Blau A Wang W Li T Kohane DS Loscalzo J Zhang YY
author_facet	Zaman P Wang J Blau A Wang W Li T Kohane DS Loscalzo J Zhang YY
author_sort	Zaman P
title	Incorporation of heparin-binding proteins into preformed dextran sulfate-chitosan nanoparticles
title_short	Incorporation of heparin-binding proteins into preformed dextran sulfate-chitosan nanoparticles
title_full	Incorporation of heparin-binding proteins into preformed dextran sulfate-chitosan nanoparticles
title_fullStr	Incorporation of heparin-binding proteins into preformed dextran sulfate-chitosan nanoparticles
title_full_unstemmed	Incorporation of heparin-binding proteins into preformed dextran sulfate-chitosan nanoparticles
title_sort	incorporation of heparin-binding proteins into preformed dextran sulfate-chitosan nanoparticles
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/6c2acd9bf99141c4beee071a1351dfbc
work_keys_str_mv	AT zamanp incorporationofheparinbindingproteinsintopreformeddextransulfatechitosannanoparticles AT wangj incorporationofheparinbindingproteinsintopreformeddextransulfatechitosannanoparticles AT blaua incorporationofheparinbindingproteinsintopreformeddextransulfatechitosannanoparticles AT wangw incorporationofheparinbindingproteinsintopreformeddextransulfatechitosannanoparticles AT lit incorporationofheparinbindingproteinsintopreformeddextransulfatechitosannanoparticles AT kohaneds incorporationofheparinbindingproteinsintopreformeddextransulfatechitosannanoparticles AT loscalzoj incorporationofheparinbindingproteinsintopreformeddextransulfatechitosannanoparticles AT zhangyy incorporationofheparinbindingproteinsintopreformeddextransulfatechitosannanoparticles
_version_	1718401555252641792

Incorporation of heparin-binding proteins into preformed dextran sulfate-chitosan nanoparticles

Ejemplares similares