TALEN outperforms Cas9 in editing heterochromatin target sites

While Cas9 outperforms TALENs in euchromatin, it is less efficient in heterochromatic regions. Here the authors, using single-molecule imaging, show that Cas9 uses a less efficient search strategy compared to TALENs in these regions.

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Autores principales:	Surbhi Jain, Saurabh Shukla, Che Yang, Meng Zhang, Zia Fatma, Manasi Lingamaneni, Shireen Abesteh, Stephan Thomas Lane, Xiong Xiong, Yuchuan Wang, Charles M. Schroeder, Paul R. Selvin, Huimin Zhao
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/6c3cb81693a24a32a018bf1763f7bcf5
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

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spelling	oai:doaj.org-article:6c3cb81693a24a32a018bf1763f7bcf52021-12-02T10:48:18ZTALEN outperforms Cas9 in editing heterochromatin target sites10.1038/s41467-020-20672-52041-1723https://doaj.org/article/6c3cb81693a24a32a018bf1763f7bcf52021-01-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-20672-5https://doaj.org/toc/2041-1723While Cas9 outperforms TALENs in euchromatin, it is less efficient in heterochromatic regions. Here the authors, using single-molecule imaging, show that Cas9 uses a less efficient search strategy compared to TALENs in these regions.Surbhi JainSaurabh ShuklaChe YangMeng ZhangZia FatmaManasi LingamaneniShireen AbestehStephan Thomas LaneXiong XiongYuchuan WangCharles M. SchroederPaul R. SelvinHuimin ZhaoNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Surbhi Jain Saurabh Shukla Che Yang Meng Zhang Zia Fatma Manasi Lingamaneni Shireen Abesteh Stephan Thomas Lane Xiong Xiong Yuchuan Wang Charles M. Schroeder Paul R. Selvin Huimin Zhao TALEN outperforms Cas9 in editing heterochromatin target sites
description	While Cas9 outperforms TALENs in euchromatin, it is less efficient in heterochromatic regions. Here the authors, using single-molecule imaging, show that Cas9 uses a less efficient search strategy compared to TALENs in these regions.
format	article
author	Surbhi Jain Saurabh Shukla Che Yang Meng Zhang Zia Fatma Manasi Lingamaneni Shireen Abesteh Stephan Thomas Lane Xiong Xiong Yuchuan Wang Charles M. Schroeder Paul R. Selvin Huimin Zhao
author_facet	Surbhi Jain Saurabh Shukla Che Yang Meng Zhang Zia Fatma Manasi Lingamaneni Shireen Abesteh Stephan Thomas Lane Xiong Xiong Yuchuan Wang Charles M. Schroeder Paul R. Selvin Huimin Zhao
author_sort	Surbhi Jain
title	TALEN outperforms Cas9 in editing heterochromatin target sites
title_short	TALEN outperforms Cas9 in editing heterochromatin target sites
title_full	TALEN outperforms Cas9 in editing heterochromatin target sites
title_fullStr	TALEN outperforms Cas9 in editing heterochromatin target sites
title_full_unstemmed	TALEN outperforms Cas9 in editing heterochromatin target sites
title_sort	talen outperforms cas9 in editing heterochromatin target sites
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/6c3cb81693a24a32a018bf1763f7bcf5
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TALEN outperforms Cas9 in editing heterochromatin target sites

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