Antibiotic resistance determinants in a Pseudomonas putida strain isolated from a hospital.

Environmental microbes harbor an enormous pool of antibiotic and biocide resistance genes that can impact the resistance profiles of animal and human pathogens via horizontal gene transfer. Pseudomonas putida strains are ubiquitous in soil and water but have been seldom isolated from humans. We have...

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Autores principales:	Lázaro Molina, Zulema Udaondo, Estrella Duque, Matilde Fernández, Carlos Molina-Santiago, Amalia Roca, Mario Porcel, Jesús de la Torre, Ana Segura, Patrick Plesiat, Katy Jeannot, Juan-Luis Ramos
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6c44e76386b847dc901dccbde2cc70f8
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spelling	oai:doaj.org-article:6c44e76386b847dc901dccbde2cc70f82021-11-18T08:37:31ZAntibiotic resistance determinants in a Pseudomonas putida strain isolated from a hospital.1932-620310.1371/journal.pone.0081604https://doaj.org/article/6c44e76386b847dc901dccbde2cc70f82014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24465371/?tool=EBIhttps://doaj.org/toc/1932-6203Environmental microbes harbor an enormous pool of antibiotic and biocide resistance genes that can impact the resistance profiles of animal and human pathogens via horizontal gene transfer. Pseudomonas putida strains are ubiquitous in soil and water but have been seldom isolated from humans. We have established a collection of P. putida strains isolated from in-patients in different hospitals in France. One of the isolated strains (HB3267) kills insects and is resistant to the majority of the antibiotics used in laboratories and hospitals, including aminoglycosides, ß-lactams, cationic peptides, chromoprotein enediyne antibiotics, dihydrofolate reductase inhibitors, fluoroquinolones and quinolones, glycopeptide antibiotics, macrolides, polyketides and sulfonamides. Similar to other P. putida clinical isolates the strain was sensitive to amikacin. To shed light on the broad pattern of antibiotic resistance, which is rarely found in clinical isolates of this species, the genome of this strain was sequenced and analysed. The study revealed that the determinants of multiple resistance are both chromosomally-borne as well as located on the pPC9 plasmid. Further analysis indicated that pPC9 has recruited antibiotic and biocide resistance genes from environmental microorganisms as well as from opportunistic and true human pathogens. The pPC9 plasmid is not self-transmissible, but can be mobilized by other bacterial plasmids making it capable of spreading antibiotic resistant determinants to new hosts.Lázaro MolinaZulema UdaondoEstrella DuqueMatilde FernándezCarlos Molina-SantiagoAmalia RocaMario PorcelJesús de la TorreAna SeguraPatrick PlesiatKaty JeannotJuan-Luis RamosPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 1, p e81604 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lázaro Molina Zulema Udaondo Estrella Duque Matilde Fernández Carlos Molina-Santiago Amalia Roca Mario Porcel Jesús de la Torre Ana Segura Patrick Plesiat Katy Jeannot Juan-Luis Ramos Antibiotic resistance determinants in a Pseudomonas putida strain isolated from a hospital.
description	Environmental microbes harbor an enormous pool of antibiotic and biocide resistance genes that can impact the resistance profiles of animal and human pathogens via horizontal gene transfer. Pseudomonas putida strains are ubiquitous in soil and water but have been seldom isolated from humans. We have established a collection of P. putida strains isolated from in-patients in different hospitals in France. One of the isolated strains (HB3267) kills insects and is resistant to the majority of the antibiotics used in laboratories and hospitals, including aminoglycosides, ß-lactams, cationic peptides, chromoprotein enediyne antibiotics, dihydrofolate reductase inhibitors, fluoroquinolones and quinolones, glycopeptide antibiotics, macrolides, polyketides and sulfonamides. Similar to other P. putida clinical isolates the strain was sensitive to amikacin. To shed light on the broad pattern of antibiotic resistance, which is rarely found in clinical isolates of this species, the genome of this strain was sequenced and analysed. The study revealed that the determinants of multiple resistance are both chromosomally-borne as well as located on the pPC9 plasmid. Further analysis indicated that pPC9 has recruited antibiotic and biocide resistance genes from environmental microorganisms as well as from opportunistic and true human pathogens. The pPC9 plasmid is not self-transmissible, but can be mobilized by other bacterial plasmids making it capable of spreading antibiotic resistant determinants to new hosts.
format	article
author	Lázaro Molina Zulema Udaondo Estrella Duque Matilde Fernández Carlos Molina-Santiago Amalia Roca Mario Porcel Jesús de la Torre Ana Segura Patrick Plesiat Katy Jeannot Juan-Luis Ramos
author_facet	Lázaro Molina Zulema Udaondo Estrella Duque Matilde Fernández Carlos Molina-Santiago Amalia Roca Mario Porcel Jesús de la Torre Ana Segura Patrick Plesiat Katy Jeannot Juan-Luis Ramos
author_sort	Lázaro Molina
title	Antibiotic resistance determinants in a Pseudomonas putida strain isolated from a hospital.
title_short	Antibiotic resistance determinants in a Pseudomonas putida strain isolated from a hospital.
title_full	Antibiotic resistance determinants in a Pseudomonas putida strain isolated from a hospital.
title_fullStr	Antibiotic resistance determinants in a Pseudomonas putida strain isolated from a hospital.
title_full_unstemmed	Antibiotic resistance determinants in a Pseudomonas putida strain isolated from a hospital.
title_sort	antibiotic resistance determinants in a pseudomonas putida strain isolated from a hospital.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/6c44e76386b847dc901dccbde2cc70f8
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Antibiotic resistance determinants in a Pseudomonas putida strain isolated from a hospital.

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