Mast cell survival and mediator secretion in response to hypoxia.

Tissue hypoxia is a consequence of decreased oxygen levels in different inflammatory conditions, many associated with mast cell activation. However, the effect of hypoxia on mast cell functions is not well established. Here, we have investigated the effect of hypoxia per se on human mast cell surviv...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Magdalena Gulliksson, Ricardo F S Carvalho, Erik Ullerås, Gunnar Nilsson
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6c5577d1d2df4c97be5292b0621f4a8b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:6c5577d1d2df4c97be5292b0621f4a8b
record_format	dspace
spelling	oai:doaj.org-article:6c5577d1d2df4c97be5292b0621f4a8b2021-11-18T06:35:48ZMast cell survival and mediator secretion in response to hypoxia.1932-620310.1371/journal.pone.0012360https://doaj.org/article/6c5577d1d2df4c97be5292b0621f4a8b2010-08-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20808808/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Tissue hypoxia is a consequence of decreased oxygen levels in different inflammatory conditions, many associated with mast cell activation. However, the effect of hypoxia on mast cell functions is not well established. Here, we have investigated the effect of hypoxia per se on human mast cell survival, mediator secretion, and reactivity. Human cord blood derived mast cells were subjected to three different culturing conditions: culture and stimulation in normoxia (21% O(2)); culture and stimulation in hypoxia (1% O(2)); or 24 hour culture in hypoxia followed by stimulation in normoxia. Hypoxia, per se, did not induce mast cell degranulation, but we observed an increased secretion of IL-6, where autocrine produced IL-6 promoted mast cell survival. Hypoxia did not have any effect on A23187 induced degranulation or secretion of cytokines. In contrast, cytokine secretion after LPS or CD30 treatment was attenuated, but not inhibited, in hypoxia compared to normoxia. Our data suggests that mast cell survival, degranulation and cytokine release are sustained under hypoxia. This may be of importance for host defence where mast cells in a hypoxic tissue can react to intruders, but also in chronic inflammations where mast cell reactivity is not inhibited by the inflammatory associated hypoxia.Magdalena GullikssonRicardo F S CarvalhoErik UlleråsGunnar NilssonPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 8, p e12360 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Magdalena Gulliksson Ricardo F S Carvalho Erik Ullerås Gunnar Nilsson Mast cell survival and mediator secretion in response to hypoxia.
description	Tissue hypoxia is a consequence of decreased oxygen levels in different inflammatory conditions, many associated with mast cell activation. However, the effect of hypoxia on mast cell functions is not well established. Here, we have investigated the effect of hypoxia per se on human mast cell survival, mediator secretion, and reactivity. Human cord blood derived mast cells were subjected to three different culturing conditions: culture and stimulation in normoxia (21% O(2)); culture and stimulation in hypoxia (1% O(2)); or 24 hour culture in hypoxia followed by stimulation in normoxia. Hypoxia, per se, did not induce mast cell degranulation, but we observed an increased secretion of IL-6, where autocrine produced IL-6 promoted mast cell survival. Hypoxia did not have any effect on A23187 induced degranulation or secretion of cytokines. In contrast, cytokine secretion after LPS or CD30 treatment was attenuated, but not inhibited, in hypoxia compared to normoxia. Our data suggests that mast cell survival, degranulation and cytokine release are sustained under hypoxia. This may be of importance for host defence where mast cells in a hypoxic tissue can react to intruders, but also in chronic inflammations where mast cell reactivity is not inhibited by the inflammatory associated hypoxia.
format	article
author	Magdalena Gulliksson Ricardo F S Carvalho Erik Ullerås Gunnar Nilsson
author_facet	Magdalena Gulliksson Ricardo F S Carvalho Erik Ullerås Gunnar Nilsson
author_sort	Magdalena Gulliksson
title	Mast cell survival and mediator secretion in response to hypoxia.
title_short	Mast cell survival and mediator secretion in response to hypoxia.
title_full	Mast cell survival and mediator secretion in response to hypoxia.
title_fullStr	Mast cell survival and mediator secretion in response to hypoxia.
title_full_unstemmed	Mast cell survival and mediator secretion in response to hypoxia.
title_sort	mast cell survival and mediator secretion in response to hypoxia.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/6c5577d1d2df4c97be5292b0621f4a8b
work_keys_str_mv	AT magdalenagulliksson mastcellsurvivalandmediatorsecretioninresponsetohypoxia AT ricardofscarvalho mastcellsurvivalandmediatorsecretioninresponsetohypoxia AT erikulleras mastcellsurvivalandmediatorsecretioninresponsetohypoxia AT gunnarnilsson mastcellsurvivalandmediatorsecretioninresponsetohypoxia
_version_	1718424420830150656

Mast cell survival and mediator secretion in response to hypoxia.

Ejemplares similares