Combination therapy with cannabidiol and chemotherapeutics in canine urothelial carcinoma cells.

<h4>Background</h4>Canine urothelial carcinoma is the most common form of canine bladder cancer. Treatment with chemotherapy has variable response rates leading to most dogs succumbing to their disease within a year. Cannabidiol is an emerging treatment within the field of oncology. In r...

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Autores principales:	Jordon M Inkol, Samuel E Hocker, Anthony J Mutsaers
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6c664f96fd7544fcb2ea5f18529eb020
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spelling	oai:doaj.org-article:6c664f96fd7544fcb2ea5f18529eb0202021-12-02T20:18:37ZCombination therapy with cannabidiol and chemotherapeutics in canine urothelial carcinoma cells.1932-620310.1371/journal.pone.0255591https://doaj.org/article/6c664f96fd7544fcb2ea5f18529eb0202021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0255591https://doaj.org/toc/1932-6203<h4>Background</h4>Canine urothelial carcinoma is the most common form of canine bladder cancer. Treatment with chemotherapy has variable response rates leading to most dogs succumbing to their disease within a year. Cannabidiol is an emerging treatment within the field of oncology. In reported in vivo studies, cannabidiol has induced apoptosis, reduced cell migration, and acted as a chemotherapy sensitizer in various human tumor types. The aim of this study was to characterize the effects of cannabidiol on canine urothelial carcinoma cell viability and apoptosis as both a single agent and in combination with chemotherapy in vitro.<h4>Results</h4>Cannabidiol reduced cell viability and induced apoptosis in canine urothelial cells as determined by crystal violet viability assay and annexin V/propidium iodide flow cytometry. Furthermore, combinations of cannabidiol with mitoxantrone and vinblastine chemotherapy yielded significantly reduced cell viability and increased apoptosis compared to single agent treatment alone. The drug interactions were deemed synergistic based on combination index calculations. Conversely, the combination of cannabidiol and carboplatin did not result in decreased cell viability and increased apoptosis compared to single agent treatment. Combination index calculations suggested an antagonistic interaction between these drugs. Finally, the combination of the non-steroidal anti-inflammatory drug piroxicam with cannabidiol did not significantly affect cell viability, although, some cell lines demonstrated decreased cell viability when mitoxantrone was combined with piroxicam.<h4>Conclusions</h4>Cannabidiol showed promising results as a single agent or in combination with mitoxantrone and vinblastine for treatment of canine urothelial carcinoma cells. Further studies are justified to investigate whether these results are translatable in vivo.Jordon M InkolSamuel E HockerAnthony J MutsaersPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 8, p e0255591 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jordon M Inkol Samuel E Hocker Anthony J Mutsaers Combination therapy with cannabidiol and chemotherapeutics in canine urothelial carcinoma cells.
description	<h4>Background</h4>Canine urothelial carcinoma is the most common form of canine bladder cancer. Treatment with chemotherapy has variable response rates leading to most dogs succumbing to their disease within a year. Cannabidiol is an emerging treatment within the field of oncology. In reported in vivo studies, cannabidiol has induced apoptosis, reduced cell migration, and acted as a chemotherapy sensitizer in various human tumor types. The aim of this study was to characterize the effects of cannabidiol on canine urothelial carcinoma cell viability and apoptosis as both a single agent and in combination with chemotherapy in vitro.<h4>Results</h4>Cannabidiol reduced cell viability and induced apoptosis in canine urothelial cells as determined by crystal violet viability assay and annexin V/propidium iodide flow cytometry. Furthermore, combinations of cannabidiol with mitoxantrone and vinblastine chemotherapy yielded significantly reduced cell viability and increased apoptosis compared to single agent treatment alone. The drug interactions were deemed synergistic based on combination index calculations. Conversely, the combination of cannabidiol and carboplatin did not result in decreased cell viability and increased apoptosis compared to single agent treatment. Combination index calculations suggested an antagonistic interaction between these drugs. Finally, the combination of the non-steroidal anti-inflammatory drug piroxicam with cannabidiol did not significantly affect cell viability, although, some cell lines demonstrated decreased cell viability when mitoxantrone was combined with piroxicam.<h4>Conclusions</h4>Cannabidiol showed promising results as a single agent or in combination with mitoxantrone and vinblastine for treatment of canine urothelial carcinoma cells. Further studies are justified to investigate whether these results are translatable in vivo.
format	article
author	Jordon M Inkol Samuel E Hocker Anthony J Mutsaers
author_facet	Jordon M Inkol Samuel E Hocker Anthony J Mutsaers
author_sort	Jordon M Inkol
title	Combination therapy with cannabidiol and chemotherapeutics in canine urothelial carcinoma cells.
title_short	Combination therapy with cannabidiol and chemotherapeutics in canine urothelial carcinoma cells.
title_full	Combination therapy with cannabidiol and chemotherapeutics in canine urothelial carcinoma cells.
title_fullStr	Combination therapy with cannabidiol and chemotherapeutics in canine urothelial carcinoma cells.
title_full_unstemmed	Combination therapy with cannabidiol and chemotherapeutics in canine urothelial carcinoma cells.
title_sort	combination therapy with cannabidiol and chemotherapeutics in canine urothelial carcinoma cells.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/6c664f96fd7544fcb2ea5f18529eb020
work_keys_str_mv	AT jordonminkol combinationtherapywithcannabidiolandchemotherapeuticsincanineurothelialcarcinomacells AT samuelehocker combinationtherapywithcannabidiolandchemotherapeuticsincanineurothelialcarcinomacells AT anthonyjmutsaers combinationtherapywithcannabidiolandchemotherapeuticsincanineurothelialcarcinomacells
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Combination therapy with cannabidiol and chemotherapeutics in canine urothelial carcinoma cells.

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