The effect of dietary supplementation with high- or low-dose omega-3 fatty acid on inflammatory pathology after traumatic brain injury in rats

This study investigated dietary supplementation as a prophylactic for neuroinflammation following traumatic brain injury (TBI) in a preclinical model. Adult male Sprague-Dawley rats received 30 days of supplementation with either water or two dietary supplements. The first consisted of high-dose ome...

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Autores principales:	Black Elise K., Phillips Jack K., Seminetta Jack, Bailes Julian, Lee John M., Finan John D.
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2021
Materias:	omega-3 fatty acid docosahexanoic acid eicosapentaenoic acid traumatic brain injury dietary supplementation neuroinflammation neuroprotection Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
Acceso en línea:	https://doaj.org/article/6c67c9c3124b43fcaaa28eab419aae7c
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spelling	oai:doaj.org-article:6c67c9c3124b43fcaaa28eab419aae7c2021-12-05T14:11:05ZThe effect of dietary supplementation with high- or low-dose omega-3 fatty acid on inflammatory pathology after traumatic brain injury in rats2081-693610.1515/tnsci-2021-0010https://doaj.org/article/6c67c9c3124b43fcaaa28eab419aae7c2021-02-01T00:00:00Zhttps://doi.org/10.1515/tnsci-2021-0010https://doaj.org/toc/2081-6936This study investigated dietary supplementation as a prophylactic for neuroinflammation following traumatic brain injury (TBI) in a preclinical model. Adult male Sprague-Dawley rats received 30 days of supplementation with either water or two dietary supplements. The first consisted of high-dose omega-3 fatty acid (O3FA) (supplement A) along with vitamin D3 and vitamin E. The second had the same ingredients at different doses with an addition of cannabidiol (supplement B). Rats were subjected to an impact TBI and then euthanized 7 days post-injury and neuroinflammation quantified by histological detection of glial fibrillary acidic protein (GFAP), a marker of astrocyte activation, and CD68, a marker of microglial activity. There was a trend toward increased GFAP staining in injured, unsupplemented animals as compared to sham, unsupplemented animals, consistent with increased activation of astrocytes in response to trauma which was reversed by supplement A but not by supplement B. The pattern of CD68 staining across groups was similar to that of GFAP staining. There was a trend toward an increase in the injured unsupplemented group, relative to sham which was reversed by supplement A but not by supplement B. CD68 staining in injured animals was concentrated in the perivascular domain. The consistency between trends across different measures of neuroinflammation showing benefits of high-dose O3FA supplementation following TBI suggests that the observed effects are real. These findings are preliminary, but they justify further study to determine the functional benefits associated with improvements in histological outcomes and understand associated dose-response curves.Black Elise K.Phillips Jack K.Seminetta JackBailes JulianLee John M.Finan John D.De Gruyterarticleomega-3 fatty aciddocosahexanoic acideicosapentaenoic acidtraumatic brain injurydietary supplementationneuroinflammationneuroprotectionNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571ENTranslational Neuroscience, Vol 12, Iss 1, Pp 76-82 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	omega-3 fatty acid docosahexanoic acid eicosapentaenoic acid traumatic brain injury dietary supplementation neuroinflammation neuroprotection Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
spellingShingle	omega-3 fatty acid docosahexanoic acid eicosapentaenoic acid traumatic brain injury dietary supplementation neuroinflammation neuroprotection Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Black Elise K. Phillips Jack K. Seminetta Jack Bailes Julian Lee John M. Finan John D. The effect of dietary supplementation with high- or low-dose omega-3 fatty acid on inflammatory pathology after traumatic brain injury in rats
description	This study investigated dietary supplementation as a prophylactic for neuroinflammation following traumatic brain injury (TBI) in a preclinical model. Adult male Sprague-Dawley rats received 30 days of supplementation with either water or two dietary supplements. The first consisted of high-dose omega-3 fatty acid (O3FA) (supplement A) along with vitamin D3 and vitamin E. The second had the same ingredients at different doses with an addition of cannabidiol (supplement B). Rats were subjected to an impact TBI and then euthanized 7 days post-injury and neuroinflammation quantified by histological detection of glial fibrillary acidic protein (GFAP), a marker of astrocyte activation, and CD68, a marker of microglial activity. There was a trend toward increased GFAP staining in injured, unsupplemented animals as compared to sham, unsupplemented animals, consistent with increased activation of astrocytes in response to trauma which was reversed by supplement A but not by supplement B. The pattern of CD68 staining across groups was similar to that of GFAP staining. There was a trend toward an increase in the injured unsupplemented group, relative to sham which was reversed by supplement A but not by supplement B. CD68 staining in injured animals was concentrated in the perivascular domain. The consistency between trends across different measures of neuroinflammation showing benefits of high-dose O3FA supplementation following TBI suggests that the observed effects are real. These findings are preliminary, but they justify further study to determine the functional benefits associated with improvements in histological outcomes and understand associated dose-response curves.
format	article
author	Black Elise K. Phillips Jack K. Seminetta Jack Bailes Julian Lee John M. Finan John D.
author_facet	Black Elise K. Phillips Jack K. Seminetta Jack Bailes Julian Lee John M. Finan John D.
author_sort	Black Elise K.
title	The effect of dietary supplementation with high- or low-dose omega-3 fatty acid on inflammatory pathology after traumatic brain injury in rats
title_short	The effect of dietary supplementation with high- or low-dose omega-3 fatty acid on inflammatory pathology after traumatic brain injury in rats
title_full	The effect of dietary supplementation with high- or low-dose omega-3 fatty acid on inflammatory pathology after traumatic brain injury in rats
title_fullStr	The effect of dietary supplementation with high- or low-dose omega-3 fatty acid on inflammatory pathology after traumatic brain injury in rats
title_full_unstemmed	The effect of dietary supplementation with high- or low-dose omega-3 fatty acid on inflammatory pathology after traumatic brain injury in rats
title_sort	effect of dietary supplementation with high- or low-dose omega-3 fatty acid on inflammatory pathology after traumatic brain injury in rats
publisher	De Gruyter
publishDate	2021
url	https://doaj.org/article/6c67c9c3124b43fcaaa28eab419aae7c
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The effect of dietary supplementation with high- or low-dose omega-3 fatty acid on inflammatory pathology after traumatic brain injury in rats

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