Transcriptome-Based Identification of Genes Responding to the Organophosphate Pesticide Phosmet in <i>Danio rerio</i>

Organophosphate pesticides (OPPs) are one of the most widely used insecticides. OPPs exert their neurotoxic effects by inhibiting acetylcholine esterase (AChE). Most of the gross developmental abnormalities observed in OPP-treated fish, on the other hand, may not be explained solely by AChE inhibiti...

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Autores principales:	Bala Murali Krishna Vasamsetti, Kyongmi Chon, Juyeong Kim, Jin-A Oh, Chang-Young Yoon, Hong-Hyun Park
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	pesticide toxicity phosmet transcriptome analysis zebrafish Genetics QH426-470
Acceso en línea:	https://doaj.org/article/6c728c2bcb714085acfa1efc9abacf44
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spelling	oai:doaj.org-article:6c728c2bcb714085acfa1efc9abacf442021-11-25T17:41:23ZTranscriptome-Based Identification of Genes Responding to the Organophosphate Pesticide Phosmet in <i>Danio rerio</i>10.3390/genes121117382073-4425https://doaj.org/article/6c728c2bcb714085acfa1efc9abacf442021-10-01T00:00:00Zhttps://www.mdpi.com/2073-4425/12/11/1738https://doaj.org/toc/2073-4425Organophosphate pesticides (OPPs) are one of the most widely used insecticides. OPPs exert their neurotoxic effects by inhibiting acetylcholine esterase (AChE). Most of the gross developmental abnormalities observed in OPP-treated fish, on the other hand, may not be explained solely by AChE inhibition. To understand the overall molecular mechanisms involved in OPP toxicity, we used the zebrafish (ZF) model. We exposed ZF embryos to an OPP, phosmet, for 96 h, and then analyzed developmental abnormalities and performed whole transcriptome analysis. Phenotypic abnormalities, such as bradycardia, spine curvature, and growth retardation, were observed in phosmet-treated ZF (PTZF). Whole transcriptome analysis revealed 2190 differentially expressed genes (DEGs), with 822 and 1368 significantly up-and downregulated genes, respectively. System process and sensory and visual perception were among the top biological pathways affected by phosmet toxicity. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed significant enrichment of metabolic pathways, calcium signaling pathway, regulation of actin cytoskeleton, cardiac muscle contraction, drug metabolism–other enzymes, and phototransduction. Quantitative real-time PCR results of six DEGs agreed with the sequencing data expression profile trend. Our findings provide insights into the consequences of phosmet exposure in ZF, as well as an estimate of the potential risk of OPPs to off-target species.Bala Murali Krishna VasamsettiKyongmi ChonJuyeong KimJin-A OhChang-Young YoonHong-Hyun ParkMDPI AGarticlepesticide toxicityphosmettranscriptome analysiszebrafishGeneticsQH426-470ENGenes, Vol 12, Iss 1738, p 1738 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	pesticide toxicity phosmet transcriptome analysis zebrafish Genetics QH426-470
spellingShingle	pesticide toxicity phosmet transcriptome analysis zebrafish Genetics QH426-470 Bala Murali Krishna Vasamsetti Kyongmi Chon Juyeong Kim Jin-A Oh Chang-Young Yoon Hong-Hyun Park Transcriptome-Based Identification of Genes Responding to the Organophosphate Pesticide Phosmet in <i>Danio rerio</i>
description	Organophosphate pesticides (OPPs) are one of the most widely used insecticides. OPPs exert their neurotoxic effects by inhibiting acetylcholine esterase (AChE). Most of the gross developmental abnormalities observed in OPP-treated fish, on the other hand, may not be explained solely by AChE inhibition. To understand the overall molecular mechanisms involved in OPP toxicity, we used the zebrafish (ZF) model. We exposed ZF embryos to an OPP, phosmet, for 96 h, and then analyzed developmental abnormalities and performed whole transcriptome analysis. Phenotypic abnormalities, such as bradycardia, spine curvature, and growth retardation, were observed in phosmet-treated ZF (PTZF). Whole transcriptome analysis revealed 2190 differentially expressed genes (DEGs), with 822 and 1368 significantly up-and downregulated genes, respectively. System process and sensory and visual perception were among the top biological pathways affected by phosmet toxicity. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed significant enrichment of metabolic pathways, calcium signaling pathway, regulation of actin cytoskeleton, cardiac muscle contraction, drug metabolism–other enzymes, and phototransduction. Quantitative real-time PCR results of six DEGs agreed with the sequencing data expression profile trend. Our findings provide insights into the consequences of phosmet exposure in ZF, as well as an estimate of the potential risk of OPPs to off-target species.
format	article
author	Bala Murali Krishna Vasamsetti Kyongmi Chon Juyeong Kim Jin-A Oh Chang-Young Yoon Hong-Hyun Park
author_facet	Bala Murali Krishna Vasamsetti Kyongmi Chon Juyeong Kim Jin-A Oh Chang-Young Yoon Hong-Hyun Park
author_sort	Bala Murali Krishna Vasamsetti
title	Transcriptome-Based Identification of Genes Responding to the Organophosphate Pesticide Phosmet in <i>Danio rerio</i>
title_short	Transcriptome-Based Identification of Genes Responding to the Organophosphate Pesticide Phosmet in <i>Danio rerio</i>
title_full	Transcriptome-Based Identification of Genes Responding to the Organophosphate Pesticide Phosmet in <i>Danio rerio</i>
title_fullStr	Transcriptome-Based Identification of Genes Responding to the Organophosphate Pesticide Phosmet in <i>Danio rerio</i>
title_full_unstemmed	Transcriptome-Based Identification of Genes Responding to the Organophosphate Pesticide Phosmet in <i>Danio rerio</i>
title_sort	transcriptome-based identification of genes responding to the organophosphate pesticide phosmet in <i>danio rerio</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/6c728c2bcb714085acfa1efc9abacf44
work_keys_str_mv	AT balamuralikrishnavasamsetti transcriptomebasedidentificationofgenesrespondingtotheorganophosphatepesticidephosmetinidaniorerioi AT kyongmichon transcriptomebasedidentificationofgenesrespondingtotheorganophosphatepesticidephosmetinidaniorerioi AT juyeongkim transcriptomebasedidentificationofgenesrespondingtotheorganophosphatepesticidephosmetinidaniorerioi AT jinaoh transcriptomebasedidentificationofgenesrespondingtotheorganophosphatepesticidephosmetinidaniorerioi AT changyoungyoon transcriptomebasedidentificationofgenesrespondingtotheorganophosphatepesticidephosmetinidaniorerioi AT honghyunpark transcriptomebasedidentificationofgenesrespondingtotheorganophosphatepesticidephosmetinidaniorerioi
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Transcriptome-Based Identification of Genes Responding to the Organophosphate Pesticide Phosmet in <i>Danio rerio</i>

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