Curcumin prevents muscle damage by regulating NF-kB and Nrf2 pathways and improves performance: an in vivo model

Kazim Sahin,1 Ragip Pala,2 Mehmet Tuzcu,3 Oguzhan Ozdemir,3 Cemal Orhan,1 Nurhan Sahin,1 Vijaya Juturu4 1Department of Animal Nutrition, Faculty of Veterinary Medicine, 2Department of Movement and Training Science, 3Department of Biology, Firat University, Elazig, Turkey; 4OmniActi...

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Autores principales:	Sahin K, Pala R, Tuzcu M, Ozdemir O, Orhan C, Sahin N, Juturu V
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	Exercise Curcumin Oxidative Stress NF-kB Nrf2 Muscle Pathology RB1-214 Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/6c75cbf780ba4317bca6d77c337ea9e8
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spelling	oai:doaj.org-article:6c75cbf780ba4317bca6d77c337ea9e82021-12-02T05:18:41ZCurcumin prevents muscle damage by regulating NF-kB and Nrf2 pathways and improves performance: an in vivo model1178-7031https://doaj.org/article/6c75cbf780ba4317bca6d77c337ea9e82016-08-01T00:00:00Zhttps://www.dovepress.com/curcumin-prevents-muscle-damage-by-regulating-nf-kb-and-nrf2-pathways--peer-reviewed-article-JIRhttps://doaj.org/toc/1178-7031Kazim Sahin,1 Ragip Pala,2 Mehmet Tuzcu,3 Oguzhan Ozdemir,3 Cemal Orhan,1 Nurhan Sahin,1 Vijaya Juturu4 1Department of Animal Nutrition, Faculty of Veterinary Medicine, 2Department of Movement and Training Science, 3Department of Biology, Firat University, Elazig, Turkey; 4OmniActive Health Technologies Inc., Morristown, NJ, USA Purpose: Exercise (Ex) increases reactive oxygen species and impairs antioxidant defense systems. Recent data suggest that curcumin (CW) possesses peroxisome proliferator-activated receptor gamma activity and anti-inflammatory properties. Therefore, this study was designed to investigate the effects of CW supplementation on Ex performance, endurance, and changes in serum and muscle proteins in rats after exhaustive Ex.Materials and methods: Twenty-eight (28) male Wistar rats (age: 8 weeks and body weight: 180±20 g) were divided into four treatment groups: 1) control (C; no Ex), 2) C + CW (no Ex + CW), 3) C + Ex, and 4) C + Ex + CW (Ex + CW). CW was administered as 100 mg/kg CurcuWin®, providing 20 mg of curcuminoids daily for 6 weeks. A motor-driven rodent treadmill was used to carry out the Ex protocols. During a 5-day period, animals in chronic Ex groups were put through different regimens: day 1, 10 m/min for 10 minutes; day 2, 20 m/min for 10 minutes; day 3, 25 m/min for 10 minutes; day 4, 25 m/min for 20 minutes; and day 5, 25 m/min for 30 minutes. Animals were exercised at 25 m/min for 45 min/d for 5 d/wk for 6 weeks. Blood and muscle samples were analyzed for muscle markers, oxidative stress, and antioxidant markers.Results: Lactate and muscle malondialdehyde levels decreased in the CW-treated groups (P<0.0001). However, activities of antioxidant enzyme levels increased in the CW-treated groups. Run to exhaustion (minutes) improved in the CW-treated groups. Muscle nuclear factor-κB (P<0.05) and heat shock protein 70 (P<0.05) levels were much lowered in the CW treated group followed by Ex group. In addition, muscle inhibitors of kappa B, peroxisome proliferator-activated receptor gamma coactivator 1-alpha, thioredoxin-1, sirtuin 1, nuclear factor (erythroid-derived 2)-like 2, and glucose transporter 4 protein levels in the Ex + CW group were higher than those in the control and Ex groups (P<0.05).Conclusion: This study suggests that novel CW has the potential to help prevent muscle damage by regulating the nuclear factor-κB and nuclear factor (erythroid-derived 2)-like 2 pathways and improve the performance and nutritional values of CW.Keywords: exercise, curcumin, oxidative stress, NF-kB, Nrf2, muscleSahin KPala RTuzcu MOzdemir OOrhan CSahin NJuturu VDove Medical PressarticleExerciseCurcuminOxidative StressNF-kBNrf2MusclePathologyRB1-214Therapeutics. PharmacologyRM1-950ENJournal of Inflammation Research, Vol Volume 9, Pp 147-154 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Exercise Curcumin Oxidative Stress NF-kB Nrf2 Muscle Pathology RB1-214 Therapeutics. Pharmacology RM1-950
spellingShingle	Exercise Curcumin Oxidative Stress NF-kB Nrf2 Muscle Pathology RB1-214 Therapeutics. Pharmacology RM1-950 Sahin K Pala R Tuzcu M Ozdemir O Orhan C Sahin N Juturu V Curcumin prevents muscle damage by regulating NF-kB and Nrf2 pathways and improves performance: an in vivo model
description	Kazim Sahin,1 Ragip Pala,2 Mehmet Tuzcu,3 Oguzhan Ozdemir,3 Cemal Orhan,1 Nurhan Sahin,1 Vijaya Juturu4 1Department of Animal Nutrition, Faculty of Veterinary Medicine, 2Department of Movement and Training Science, 3Department of Biology, Firat University, Elazig, Turkey; 4OmniActive Health Technologies Inc., Morristown, NJ, USA Purpose: Exercise (Ex) increases reactive oxygen species and impairs antioxidant defense systems. Recent data suggest that curcumin (CW) possesses peroxisome proliferator-activated receptor gamma activity and anti-inflammatory properties. Therefore, this study was designed to investigate the effects of CW supplementation on Ex performance, endurance, and changes in serum and muscle proteins in rats after exhaustive Ex.Materials and methods: Twenty-eight (28) male Wistar rats (age: 8 weeks and body weight: 180±20 g) were divided into four treatment groups: 1) control (C; no Ex), 2) C + CW (no Ex + CW), 3) C + Ex, and 4) C + Ex + CW (Ex + CW). CW was administered as 100 mg/kg CurcuWin®, providing 20 mg of curcuminoids daily for 6 weeks. A motor-driven rodent treadmill was used to carry out the Ex protocols. During a 5-day period, animals in chronic Ex groups were put through different regimens: day 1, 10 m/min for 10 minutes; day 2, 20 m/min for 10 minutes; day 3, 25 m/min for 10 minutes; day 4, 25 m/min for 20 minutes; and day 5, 25 m/min for 30 minutes. Animals were exercised at 25 m/min for 45 min/d for 5 d/wk for 6 weeks. Blood and muscle samples were analyzed for muscle markers, oxidative stress, and antioxidant markers.Results: Lactate and muscle malondialdehyde levels decreased in the CW-treated groups (P<0.0001). However, activities of antioxidant enzyme levels increased in the CW-treated groups. Run to exhaustion (minutes) improved in the CW-treated groups. Muscle nuclear factor-κB (P<0.05) and heat shock protein 70 (P<0.05) levels were much lowered in the CW treated group followed by Ex group. In addition, muscle inhibitors of kappa B, peroxisome proliferator-activated receptor gamma coactivator 1-alpha, thioredoxin-1, sirtuin 1, nuclear factor (erythroid-derived 2)-like 2, and glucose transporter 4 protein levels in the Ex + CW group were higher than those in the control and Ex groups (P<0.05).Conclusion: This study suggests that novel CW has the potential to help prevent muscle damage by regulating the nuclear factor-κB and nuclear factor (erythroid-derived 2)-like 2 pathways and improve the performance and nutritional values of CW.Keywords: exercise, curcumin, oxidative stress, NF-kB, Nrf2, muscle
format	article
author	Sahin K Pala R Tuzcu M Ozdemir O Orhan C Sahin N Juturu V
author_facet	Sahin K Pala R Tuzcu M Ozdemir O Orhan C Sahin N Juturu V
author_sort	Sahin K
title	Curcumin prevents muscle damage by regulating NF-kB and Nrf2 pathways and improves performance: an in vivo model
title_short	Curcumin prevents muscle damage by regulating NF-kB and Nrf2 pathways and improves performance: an in vivo model
title_full	Curcumin prevents muscle damage by regulating NF-kB and Nrf2 pathways and improves performance: an in vivo model
title_fullStr	Curcumin prevents muscle damage by regulating NF-kB and Nrf2 pathways and improves performance: an in vivo model
title_full_unstemmed	Curcumin prevents muscle damage by regulating NF-kB and Nrf2 pathways and improves performance: an in vivo model
title_sort	curcumin prevents muscle damage by regulating nf-kb and nrf2 pathways and improves performance: an in vivo model
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/6c75cbf780ba4317bca6d77c337ea9e8
work_keys_str_mv	AT sahink curcuminpreventsmuscledamagebyregulatingnfkbandnrf2pathwaysandimprovesperformanceaninvivomodel AT palar curcuminpreventsmuscledamagebyregulatingnfkbandnrf2pathwaysandimprovesperformanceaninvivomodel AT tuzcum curcuminpreventsmuscledamagebyregulatingnfkbandnrf2pathwaysandimprovesperformanceaninvivomodel AT ozdemiro curcuminpreventsmuscledamagebyregulatingnfkbandnrf2pathwaysandimprovesperformanceaninvivomodel AT orhanc curcuminpreventsmuscledamagebyregulatingnfkbandnrf2pathwaysandimprovesperformanceaninvivomodel AT sahinn curcuminpreventsmuscledamagebyregulatingnfkbandnrf2pathwaysandimprovesperformanceaninvivomodel AT juturuv curcuminpreventsmuscledamagebyregulatingnfkbandnrf2pathwaysandimprovesperformanceaninvivomodel
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Curcumin prevents muscle damage by regulating NF-kB and Nrf2 pathways and improves performance: an in vivo model

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