Delayed effects of transcriptional responses in Mycobacterium tuberculosis exposed to nitric oxide suggest other mechanisms involved in survival

Abstract Mycobacterium tuberculosis has succeeded as a human pathogen for tens of thousands of years thanks to its ability to resist and adapt to the adverse conditions it encounters upon infection. Bacterial adaptation to stress is commonly viewed in the context of transcriptional regulation, with...

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Autores principales:	Teresa Cortes, Olga T. Schubert, Amir Banaei-Esfahani, Ben C. Collins, Ruedi Aebersold, Douglas B. Young
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6c777e17ef37415aa7763f9a16e8e9b1
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spelling	oai:doaj.org-article:6c777e17ef37415aa7763f9a16e8e9b12021-12-02T16:06:22ZDelayed effects of transcriptional responses in Mycobacterium tuberculosis exposed to nitric oxide suggest other mechanisms involved in survival10.1038/s41598-017-08306-12045-2322https://doaj.org/article/6c777e17ef37415aa7763f9a16e8e9b12017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08306-1https://doaj.org/toc/2045-2322Abstract Mycobacterium tuberculosis has succeeded as a human pathogen for tens of thousands of years thanks to its ability to resist and adapt to the adverse conditions it encounters upon infection. Bacterial adaptation to stress is commonly viewed in the context of transcriptional regulation, with the implicit expectation that an initial transcriptomic response is tightly coupled to an ensuing proteomic response. However, after challenging M. tuberculosis with nitric oxide we found that the rapid transcriptional responses, detectable within minutes of nitric oxide exposure, typically took several hours to manifest on the protein level. Furthermore, early proteomic responses were dominated by the degradation of a set of proteins, specifically those containing damaged iron-sulphur clusters. Overall, our findings are consistent with transcriptional responses participating mostly in late-stage recovery rather than in generating an immediate resistance to nitric oxide stress, suggesting that survival of M. tuberculosis under acute stress is contingent on mechanisms other than transcriptional regulation. These findings provide a revised molecular understanding of an important human pathogen.Teresa CortesOlga T. SchubertAmir Banaei-EsfahaniBen C. CollinsRuedi AebersoldDouglas B. YoungNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Teresa Cortes Olga T. Schubert Amir Banaei-Esfahani Ben C. Collins Ruedi Aebersold Douglas B. Young Delayed effects of transcriptional responses in Mycobacterium tuberculosis exposed to nitric oxide suggest other mechanisms involved in survival
description	Abstract Mycobacterium tuberculosis has succeeded as a human pathogen for tens of thousands of years thanks to its ability to resist and adapt to the adverse conditions it encounters upon infection. Bacterial adaptation to stress is commonly viewed in the context of transcriptional regulation, with the implicit expectation that an initial transcriptomic response is tightly coupled to an ensuing proteomic response. However, after challenging M. tuberculosis with nitric oxide we found that the rapid transcriptional responses, detectable within minutes of nitric oxide exposure, typically took several hours to manifest on the protein level. Furthermore, early proteomic responses were dominated by the degradation of a set of proteins, specifically those containing damaged iron-sulphur clusters. Overall, our findings are consistent with transcriptional responses participating mostly in late-stage recovery rather than in generating an immediate resistance to nitric oxide stress, suggesting that survival of M. tuberculosis under acute stress is contingent on mechanisms other than transcriptional regulation. These findings provide a revised molecular understanding of an important human pathogen.
format	article
author	Teresa Cortes Olga T. Schubert Amir Banaei-Esfahani Ben C. Collins Ruedi Aebersold Douglas B. Young
author_facet	Teresa Cortes Olga T. Schubert Amir Banaei-Esfahani Ben C. Collins Ruedi Aebersold Douglas B. Young
author_sort	Teresa Cortes
title	Delayed effects of transcriptional responses in Mycobacterium tuberculosis exposed to nitric oxide suggest other mechanisms involved in survival
title_short	Delayed effects of transcriptional responses in Mycobacterium tuberculosis exposed to nitric oxide suggest other mechanisms involved in survival
title_full	Delayed effects of transcriptional responses in Mycobacterium tuberculosis exposed to nitric oxide suggest other mechanisms involved in survival
title_fullStr	Delayed effects of transcriptional responses in Mycobacterium tuberculosis exposed to nitric oxide suggest other mechanisms involved in survival
title_full_unstemmed	Delayed effects of transcriptional responses in Mycobacterium tuberculosis exposed to nitric oxide suggest other mechanisms involved in survival
title_sort	delayed effects of transcriptional responses in mycobacterium tuberculosis exposed to nitric oxide suggest other mechanisms involved in survival
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/6c777e17ef37415aa7763f9a16e8e9b1
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Delayed effects of transcriptional responses in Mycobacterium tuberculosis exposed to nitric oxide suggest other mechanisms involved in survival

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