Identification of autoreactive B cells with labeled nucleosomes

Abstract The pathogenesis of autoimmune diseases has not been completely elucidated yet, and only a few specific treatments have been developed so far. In autoimmune diseases mediated by pathogenic autoantibodies, such as systemic lupus erythematosus, the specific detection and analysis of autoreact...

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Autores principales:	Vincent Gies, Alain Wagner, Cécile Seifert, Aurélien Guffroy, Jean-D. Fauny, Anne-M. Knapp, Jean-L. Pasquali, Thierry Martin, Hélène Dumortier, Anne-S. Korganow, Pauline Soulas-Sprauel
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6c77ffbdd46a4c88b289d491d3de3d79
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spelling	oai:doaj.org-article:6c77ffbdd46a4c88b289d491d3de3d792021-12-02T11:52:19ZIdentification of autoreactive B cells with labeled nucleosomes10.1038/s41598-017-00664-02045-2322https://doaj.org/article/6c77ffbdd46a4c88b289d491d3de3d792017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00664-0https://doaj.org/toc/2045-2322Abstract The pathogenesis of autoimmune diseases has not been completely elucidated yet, and only a few specific treatments have been developed so far. In autoimmune diseases mediated by pathogenic autoantibodies, such as systemic lupus erythematosus, the specific detection and analysis of autoreactive B cells is crucial for a better understanding of the physiopathology. Biological characterization of these cells may help to define new therapeutic targets. Very few techniques allowing the precise detection of autoreactive B cells have been described so far. Herein we propose a new flow cytometry technique for specific detection of anti-nucleosome B cells, which secrete autoantibodies in systemic lupus erythematosus, using labeled nucleosomes. We produced different fluorochrome-labeled nucleosomes, characterized them, and finally tested them in flow cytometry. Nucleosomes labeled via the cysteines present in H3 histone specifically bind to autoreactive B cells in the anti-DNA transgenic B6.56R mice model. The present work validates the use of fluorochrome-labeled nucleosomes via cysteines to identify anti-nucleosome B cells and offers new opportunities for the description of autoreactive B cell phenotype.Vincent GiesAlain WagnerCécile SeifertAurélien GuffroyJean-D. FaunyAnne-M. KnappJean-L. PasqualiThierry MartinHélène DumortierAnne-S. KorganowPauline Soulas-SprauelNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Vincent Gies Alain Wagner Cécile Seifert Aurélien Guffroy Jean-D. Fauny Anne-M. Knapp Jean-L. Pasquali Thierry Martin Hélène Dumortier Anne-S. Korganow Pauline Soulas-Sprauel Identification of autoreactive B cells with labeled nucleosomes
description	Abstract The pathogenesis of autoimmune diseases has not been completely elucidated yet, and only a few specific treatments have been developed so far. In autoimmune diseases mediated by pathogenic autoantibodies, such as systemic lupus erythematosus, the specific detection and analysis of autoreactive B cells is crucial for a better understanding of the physiopathology. Biological characterization of these cells may help to define new therapeutic targets. Very few techniques allowing the precise detection of autoreactive B cells have been described so far. Herein we propose a new flow cytometry technique for specific detection of anti-nucleosome B cells, which secrete autoantibodies in systemic lupus erythematosus, using labeled nucleosomes. We produced different fluorochrome-labeled nucleosomes, characterized them, and finally tested them in flow cytometry. Nucleosomes labeled via the cysteines present in H3 histone specifically bind to autoreactive B cells in the anti-DNA transgenic B6.56R mice model. The present work validates the use of fluorochrome-labeled nucleosomes via cysteines to identify anti-nucleosome B cells and offers new opportunities for the description of autoreactive B cell phenotype.
format	article
author	Vincent Gies Alain Wagner Cécile Seifert Aurélien Guffroy Jean-D. Fauny Anne-M. Knapp Jean-L. Pasquali Thierry Martin Hélène Dumortier Anne-S. Korganow Pauline Soulas-Sprauel
author_facet	Vincent Gies Alain Wagner Cécile Seifert Aurélien Guffroy Jean-D. Fauny Anne-M. Knapp Jean-L. Pasquali Thierry Martin Hélène Dumortier Anne-S. Korganow Pauline Soulas-Sprauel
author_sort	Vincent Gies
title	Identification of autoreactive B cells with labeled nucleosomes
title_short	Identification of autoreactive B cells with labeled nucleosomes
title_full	Identification of autoreactive B cells with labeled nucleosomes
title_fullStr	Identification of autoreactive B cells with labeled nucleosomes
title_full_unstemmed	Identification of autoreactive B cells with labeled nucleosomes
title_sort	identification of autoreactive b cells with labeled nucleosomes
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/6c77ffbdd46a4c88b289d491d3de3d79
work_keys_str_mv	AT vincentgies identificationofautoreactivebcellswithlabelednucleosomes AT alainwagner identificationofautoreactivebcellswithlabelednucleosomes AT cecileseifert identificationofautoreactivebcellswithlabelednucleosomes AT aurelienguffroy identificationofautoreactivebcellswithlabelednucleosomes AT jeandfauny identificationofautoreactivebcellswithlabelednucleosomes AT annemknapp identificationofautoreactivebcellswithlabelednucleosomes AT jeanlpasquali identificationofautoreactivebcellswithlabelednucleosomes AT thierrymartin identificationofautoreactivebcellswithlabelednucleosomes AT helenedumortier identificationofautoreactivebcellswithlabelednucleosomes AT anneskorganow identificationofautoreactivebcellswithlabelednucleosomes AT paulinesoulassprauel identificationofautoreactivebcellswithlabelednucleosomes
_version_	1718395108079960064

Identification of autoreactive B cells with labeled nucleosomes

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