Chlamydia pecorum Ovine Abortion: Associations between Maternal Infection and Perinatal Mortality

Chlamydia pecorum is a common gastrointestinal inhabitant of livestock but infections can manifest in a broad array of clinical presentations and in a range of host species. While C. pecorum is a known cause of ovine abortion, clinical cases have only recently b...

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Autores principales:	Cheryl Jenkins, Martina Jelocnik, Emily Onizawa, Justine McNally, Ronald Coilparampil, Pedro Pinczowski, Daniel Bogema, Thomas Westermann
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Chlamydia pecorum</i> abortion ovine serology ST23 plasmid Medicine R
Acceso en línea:	https://doaj.org/article/6c9bb863f85d4fcdbb72ce7213033b18
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spelling	oai:doaj.org-article:6c9bb863f85d4fcdbb72ce7213033b182021-11-25T18:37:40Z<i>Chlamydia pecorum</i> Ovine Abortion: Associations between Maternal Infection and Perinatal Mortality10.3390/pathogens101113672076-0817https://doaj.org/article/6c9bb863f85d4fcdbb72ce7213033b182021-10-01T00:00:00Zhttps://www.mdpi.com/2076-0817/10/11/1367https://doaj.org/toc/2076-0817<i>Chlamydia pecorum</i> is a common gastrointestinal inhabitant of livestock but infections can manifest in a broad array of clinical presentations and in a range of host species. While <i>C. pecorum</i> is a known cause of ovine abortion, clinical cases have only recently been described in detail. Here, the prevalence and sequence types (STs) of <i>C. pecorum</i> in ewes from a property experiencing high levels of perinatal mortality (PNM) in New South Wales (NSW), Australia, were investigated using serological and molecular methods. Ewes that were PNM+ were statistically more likely to test seropositive compared to PNM− ewes and displayed higher antibody titres; however, an increase in chlamydial shedding from either the rectum, vagina or conjunctiva of PNM+ ewes was not observed. Multilocus sequence typing (MLST) indicated that <i>C. pecorum</i> ST23 was the major ST shed by ewes in the flock, was the only ST identified from the vaginal site, and was the same ST detected within aborted foetal tissues. Whole genome sequencing of <i>C. pecorum</i> isolated from one abortion case revealed that the <i>C. pecorum</i> plasmid (pCpec) contained a unique deletion in coding sequence 1 (CDS1) that was also present in <i>C. pecorum</i> ST23 shed from the ewes. A further unique deletion was noted in a polymorphic membrane protein gene (pmpG) of the <i>C. pecorum</i> chromosome, which warrants further investigation given the role of PmpG in host cell adherence and tissue tropism.This study describes novel infection parameters in a sheep flock experiencing <i>C. pecorum</i>-associated perinatal mortality, provides the first genomic data from an abortigenic <i>C. pecorum</i> strain, and raises questions about possible links between unique genetic features of this strain and <i>C. pecorum</i> abortion.Cheryl JenkinsMartina JelocnikEmily OnizawaJustine McNallyRonald CoilparampilPedro PinczowskiDaniel BogemaThomas WestermannMDPI AGarticle<i>Chlamydia pecorum</i>abortionovineserologyST23plasmidMedicineRENPathogens, Vol 10, Iss 1367, p 1367 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Chlamydia pecorum</i> abortion ovine serology ST23 plasmid Medicine R
spellingShingle	<i>Chlamydia pecorum</i> abortion ovine serology ST23 plasmid Medicine R Cheryl Jenkins Martina Jelocnik Emily Onizawa Justine McNally Ronald Coilparampil Pedro Pinczowski Daniel Bogema Thomas Westermann <i>Chlamydia pecorum</i> Ovine Abortion: Associations between Maternal Infection and Perinatal Mortality
description	<i>Chlamydia pecorum</i> is a common gastrointestinal inhabitant of livestock but infections can manifest in a broad array of clinical presentations and in a range of host species. While <i>C. pecorum</i> is a known cause of ovine abortion, clinical cases have only recently been described in detail. Here, the prevalence and sequence types (STs) of <i>C. pecorum</i> in ewes from a property experiencing high levels of perinatal mortality (PNM) in New South Wales (NSW), Australia, were investigated using serological and molecular methods. Ewes that were PNM+ were statistically more likely to test seropositive compared to PNM− ewes and displayed higher antibody titres; however, an increase in chlamydial shedding from either the rectum, vagina or conjunctiva of PNM+ ewes was not observed. Multilocus sequence typing (MLST) indicated that <i>C. pecorum</i> ST23 was the major ST shed by ewes in the flock, was the only ST identified from the vaginal site, and was the same ST detected within aborted foetal tissues. Whole genome sequencing of <i>C. pecorum</i> isolated from one abortion case revealed that the <i>C. pecorum</i> plasmid (pCpec) contained a unique deletion in coding sequence 1 (CDS1) that was also present in <i>C. pecorum</i> ST23 shed from the ewes. A further unique deletion was noted in a polymorphic membrane protein gene (pmpG) of the <i>C. pecorum</i> chromosome, which warrants further investigation given the role of PmpG in host cell adherence and tissue tropism.This study describes novel infection parameters in a sheep flock experiencing <i>C. pecorum</i>-associated perinatal mortality, provides the first genomic data from an abortigenic <i>C. pecorum</i> strain, and raises questions about possible links between unique genetic features of this strain and <i>C. pecorum</i> abortion.
format	article
author	Cheryl Jenkins Martina Jelocnik Emily Onizawa Justine McNally Ronald Coilparampil Pedro Pinczowski Daniel Bogema Thomas Westermann
author_facet	Cheryl Jenkins Martina Jelocnik Emily Onizawa Justine McNally Ronald Coilparampil Pedro Pinczowski Daniel Bogema Thomas Westermann
author_sort	Cheryl Jenkins
title	<i>Chlamydia pecorum</i> Ovine Abortion: Associations between Maternal Infection and Perinatal Mortality
title_short	<i>Chlamydia pecorum</i> Ovine Abortion: Associations between Maternal Infection and Perinatal Mortality
title_full	<i>Chlamydia pecorum</i> Ovine Abortion: Associations between Maternal Infection and Perinatal Mortality
title_fullStr	<i>Chlamydia pecorum</i> Ovine Abortion: Associations between Maternal Infection and Perinatal Mortality
title_full_unstemmed	<i>Chlamydia pecorum</i> Ovine Abortion: Associations between Maternal Infection and Perinatal Mortality
title_sort	<i>chlamydia pecorum</i> ovine abortion: associations between maternal infection and perinatal mortality
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/6c9bb863f85d4fcdbb72ce7213033b18
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<i>Chlamydia pecorum</i> Ovine Abortion: Associations between Maternal Infection and Perinatal Mortality

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