Experimental study of the effects of hypoxia simulator on osteointegration of titanium prosthesis in osteoporotic rats

Abstract Background Poor osseointegration is the key reason for implant failure after arthroplasty,whether under osteoporotic or normal bone conditions. To date, osseointegration remains a major challenge. Recent studies have shown that deferoxamine (DFO) can accelerate osteogenesis by activating th...

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Autores principales:	Jiangfeng Liu, Huijun Kang, Jiangfeng Lu, Yike Dai, Fei Wang
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Deferoxamine Osseointegration Osteoporosis Arthroplasty Titanium prosthesis Diseases of the musculoskeletal system RC925-935
Acceso en línea:	https://doaj.org/article/6c9ca311d0ca4a0da4ce341bc23a0de0
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spelling	oai:doaj.org-article:6c9ca311d0ca4a0da4ce341bc23a0de02021-11-14T12:28:24ZExperimental study of the effects of hypoxia simulator on osteointegration of titanium prosthesis in osteoporotic rats10.1186/s12891-021-04777-61471-2474https://doaj.org/article/6c9ca311d0ca4a0da4ce341bc23a0de02021-11-01T00:00:00Zhttps://doi.org/10.1186/s12891-021-04777-6https://doaj.org/toc/1471-2474Abstract Background Poor osseointegration is the key reason for implant failure after arthroplasty,whether under osteoporotic or normal bone conditions. To date, osseointegration remains a major challenge. Recent studies have shown that deferoxamine (DFO) can accelerate osteogenesis by activating the hypoxia signaling pathway. The purpose of this study was to test the following hypothesis: after knee replacement, intra-articular injection of DFO will promote osteogenesis and osseointegration with a 3D printed titanium prosthesis in the bones of osteoporotic rats. Materials and methods Ninety female Sprague–Dawley rats were used for the experiment. Ten rats were used to confirm the successful establishment of the osteoporosis model: five rats in the sham operation group and five rats in the ovariectomy group. After ovariectomy and knee arthroplasty were performed, the remaining 80 rats were randomly divided into DFO and control groups (n = 40 per group). The two groups were treated by intraarticular injection of DFO and saline respectively. After 2 weeks, polymerase chain reaction (PCR) and immunohistochemistry were used to evaluate the levels of HIF-1a, VEGF, and CD31. HIF-1a and VEGF have been shown to promote angiogenesis and bone regeneration, and CD31 is an important marker of angiogenesis. After 12 weeks, the specimens were examined by micro-computed tomography (micro-CT), biomechanics, and histopathology to evaluate osteogenesis and osseointegration. Results The results of PCR showed that the mRNA levels of VEGF and CD31 in the DFO group were significantly higher than those in the control group. The immunohistochemistry results indicated that positive cell expression of HIF-1a, VEGF, and CD31 in the DFO group was also higher. Compared with the control group, the micro-CT parameters of BMD, BV/TV, TB. N, and TB. Th were significantly higher. The maximal pull-out force and the bone-to-implant contact value were also higher. Conclusions The local administration of DFO, which is used to activate the HIF-1a signaling pathway, can promote osteogenesis and osseointegration with a prosthesis in osteoporotic bone.Jiangfeng LiuHuijun KangJiangfeng LuYike DaiFei WangBMCarticleDeferoxamineOsseointegrationOsteoporosisArthroplastyTitanium prosthesisDiseases of the musculoskeletal systemRC925-935ENBMC Musculoskeletal Disorders, Vol 22, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Deferoxamine Osseointegration Osteoporosis Arthroplasty Titanium prosthesis Diseases of the musculoskeletal system RC925-935
spellingShingle	Deferoxamine Osseointegration Osteoporosis Arthroplasty Titanium prosthesis Diseases of the musculoskeletal system RC925-935 Jiangfeng Liu Huijun Kang Jiangfeng Lu Yike Dai Fei Wang Experimental study of the effects of hypoxia simulator on osteointegration of titanium prosthesis in osteoporotic rats
description	Abstract Background Poor osseointegration is the key reason for implant failure after arthroplasty,whether under osteoporotic or normal bone conditions. To date, osseointegration remains a major challenge. Recent studies have shown that deferoxamine (DFO) can accelerate osteogenesis by activating the hypoxia signaling pathway. The purpose of this study was to test the following hypothesis: after knee replacement, intra-articular injection of DFO will promote osteogenesis and osseointegration with a 3D printed titanium prosthesis in the bones of osteoporotic rats. Materials and methods Ninety female Sprague–Dawley rats were used for the experiment. Ten rats were used to confirm the successful establishment of the osteoporosis model: five rats in the sham operation group and five rats in the ovariectomy group. After ovariectomy and knee arthroplasty were performed, the remaining 80 rats were randomly divided into DFO and control groups (n = 40 per group). The two groups were treated by intraarticular injection of DFO and saline respectively. After 2 weeks, polymerase chain reaction (PCR) and immunohistochemistry were used to evaluate the levels of HIF-1a, VEGF, and CD31. HIF-1a and VEGF have been shown to promote angiogenesis and bone regeneration, and CD31 is an important marker of angiogenesis. After 12 weeks, the specimens were examined by micro-computed tomography (micro-CT), biomechanics, and histopathology to evaluate osteogenesis and osseointegration. Results The results of PCR showed that the mRNA levels of VEGF and CD31 in the DFO group were significantly higher than those in the control group. The immunohistochemistry results indicated that positive cell expression of HIF-1a, VEGF, and CD31 in the DFO group was also higher. Compared with the control group, the micro-CT parameters of BMD, BV/TV, TB. N, and TB. Th were significantly higher. The maximal pull-out force and the bone-to-implant contact value were also higher. Conclusions The local administration of DFO, which is used to activate the HIF-1a signaling pathway, can promote osteogenesis and osseointegration with a prosthesis in osteoporotic bone.
format	article
author	Jiangfeng Liu Huijun Kang Jiangfeng Lu Yike Dai Fei Wang
author_facet	Jiangfeng Liu Huijun Kang Jiangfeng Lu Yike Dai Fei Wang
author_sort	Jiangfeng Liu
title	Experimental study of the effects of hypoxia simulator on osteointegration of titanium prosthesis in osteoporotic rats
title_short	Experimental study of the effects of hypoxia simulator on osteointegration of titanium prosthesis in osteoporotic rats
title_full	Experimental study of the effects of hypoxia simulator on osteointegration of titanium prosthesis in osteoporotic rats
title_fullStr	Experimental study of the effects of hypoxia simulator on osteointegration of titanium prosthesis in osteoporotic rats
title_full_unstemmed	Experimental study of the effects of hypoxia simulator on osteointegration of titanium prosthesis in osteoporotic rats
title_sort	experimental study of the effects of hypoxia simulator on osteointegration of titanium prosthesis in osteoporotic rats
publisher	BMC
publishDate	2021
url	https://doaj.org/article/6c9ca311d0ca4a0da4ce341bc23a0de0
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Experimental study of the effects of hypoxia simulator on osteointegration of titanium prosthesis in osteoporotic rats

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