Development of High-Throughput Multiplex Serology to Detect Serum Antibodies against Coxiella burnetii

The causative agent of Q fever, the bacterium Coxiella burnetii (C. burnetii), has gained increasing interest due to outbreak events and reports about it being a potential risk factor for the development of lymphomas. In order to conduct large-scale studies for...

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Autores principales:	Rima Jeske, Larissa Dangel, Leander Sauerbrey, Dimitrios Frangoulidis, Lauren R. Teras, Silke F. Fischer, Tim Waterboer
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Coxiella burnetii</i> multiplex serology seroepidemiology infection marker Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/6ca68918621246378e37c5e6a917ce42
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spelling	oai:doaj.org-article:6ca68918621246378e37c5e6a917ce422021-11-25T18:25:30ZDevelopment of High-Throughput Multiplex Serology to Detect Serum Antibodies against <i>Coxiella burnetii</i>10.3390/microorganisms91123732076-2607https://doaj.org/article/6ca68918621246378e37c5e6a917ce422021-11-01T00:00:00Zhttps://www.mdpi.com/2076-2607/9/11/2373https://doaj.org/toc/2076-2607The causative agent of Q fever, the bacterium <i>Coxiella burnetii</i> (<i>C. burnetii</i>), has gained increasing interest due to outbreak events and reports about it being a potential risk factor for the development of lymphomas. In order to conduct large-scale studies for population monitoring and to investigate possible associations more closely, accurate and cost-effective high-throughput assays are highly desired. To address this need, nine <i>C. burnetii</i> proteins were expressed as recombinant antigens for multiplex serology. This technique enables the quantitative high-throughput detection of antibodies to multiple antigens simultaneously in a single reaction. Based on a reference group of 76 seropositive and 91 seronegative sera, three antigens were able to detect <i>C. burnetii</i> infections. Com1, GroEL, and DnaK achieved specificities of 93%, 69%, and 77% and sensitivities of 64%, 72%, and 47%, respectively. Double positivity to Com1 and GroEL led to a combined specificity of 90% and a sensitivity of 71%. In a subgroup of seropositives with an increased risk for chronic Q fever, the double positivity to these markers reached a specificity of 90% and a sensitivity of 86%. Multiplex serology enables the detection of antibodies against <i>C. burnetii</i> and appears well-suited to investigate associations between <i>C. burnetii</i> infections and the clinical manifestations in large-scale studies.Rima JeskeLarissa DangelLeander SauerbreyDimitrios FrangoulidisLauren R. TerasSilke F. FischerTim WaterboerMDPI AGarticle<i>Coxiella burnetii</i>multiplex serologyseroepidemiologyinfection markerBiology (General)QH301-705.5ENMicroorganisms, Vol 9, Iss 2373, p 2373 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Coxiella burnetii</i> multiplex serology seroepidemiology infection marker Biology (General) QH301-705.5
spellingShingle	<i>Coxiella burnetii</i> multiplex serology seroepidemiology infection marker Biology (General) QH301-705.5 Rima Jeske Larissa Dangel Leander Sauerbrey Dimitrios Frangoulidis Lauren R. Teras Silke F. Fischer Tim Waterboer Development of High-Throughput Multiplex Serology to Detect Serum Antibodies against <i>Coxiella burnetii</i>
description	The causative agent of Q fever, the bacterium <i>Coxiella burnetii</i> (<i>C. burnetii</i>), has gained increasing interest due to outbreak events and reports about it being a potential risk factor for the development of lymphomas. In order to conduct large-scale studies for population monitoring and to investigate possible associations more closely, accurate and cost-effective high-throughput assays are highly desired. To address this need, nine <i>C. burnetii</i> proteins were expressed as recombinant antigens for multiplex serology. This technique enables the quantitative high-throughput detection of antibodies to multiple antigens simultaneously in a single reaction. Based on a reference group of 76 seropositive and 91 seronegative sera, three antigens were able to detect <i>C. burnetii</i> infections. Com1, GroEL, and DnaK achieved specificities of 93%, 69%, and 77% and sensitivities of 64%, 72%, and 47%, respectively. Double positivity to Com1 and GroEL led to a combined specificity of 90% and a sensitivity of 71%. In a subgroup of seropositives with an increased risk for chronic Q fever, the double positivity to these markers reached a specificity of 90% and a sensitivity of 86%. Multiplex serology enables the detection of antibodies against <i>C. burnetii</i> and appears well-suited to investigate associations between <i>C. burnetii</i> infections and the clinical manifestations in large-scale studies.
format	article
author	Rima Jeske Larissa Dangel Leander Sauerbrey Dimitrios Frangoulidis Lauren R. Teras Silke F. Fischer Tim Waterboer
author_facet	Rima Jeske Larissa Dangel Leander Sauerbrey Dimitrios Frangoulidis Lauren R. Teras Silke F. Fischer Tim Waterboer
author_sort	Rima Jeske
title	Development of High-Throughput Multiplex Serology to Detect Serum Antibodies against <i>Coxiella burnetii</i>
title_short	Development of High-Throughput Multiplex Serology to Detect Serum Antibodies against <i>Coxiella burnetii</i>
title_full	Development of High-Throughput Multiplex Serology to Detect Serum Antibodies against <i>Coxiella burnetii</i>
title_fullStr	Development of High-Throughput Multiplex Serology to Detect Serum Antibodies against <i>Coxiella burnetii</i>
title_full_unstemmed	Development of High-Throughput Multiplex Serology to Detect Serum Antibodies against <i>Coxiella burnetii</i>
title_sort	development of high-throughput multiplex serology to detect serum antibodies against <i>coxiella burnetii</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/6ca68918621246378e37c5e6a917ce42
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Development of High-Throughput Multiplex Serology to Detect Serum Antibodies against <i>Coxiella burnetii</i>

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