DNA Self-assembly Catalyzed by Artificial Agents

Abstract Nucleic acids have been shown to be versatile molecules and engineered to produce various nanostructures. However, the poor rate of these uncatalyzed nucleic acid reactions has restricted the development and applications. Herein, we reported a novel finding that DNA self-assembly could be n...

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Autores principales:	Chao Shi, Yifan Wang, Menghua Zhang, Cuiping Ma
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6ca7fd9b0a054bc7a9610cd2b750a439
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spelling	oai:doaj.org-article:6ca7fd9b0a054bc7a9610cd2b750a4392021-12-02T11:40:45ZDNA Self-assembly Catalyzed by Artificial Agents10.1038/s41598-017-07210-y2045-2322https://doaj.org/article/6ca7fd9b0a054bc7a9610cd2b750a4392017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07210-yhttps://doaj.org/toc/2045-2322Abstract Nucleic acids have been shown to be versatile molecules and engineered to produce various nanostructures. However, the poor rate of these uncatalyzed nucleic acid reactions has restricted the development and applications. Herein, we reported a novel finding that DNA self-assembly could be nonenzymatically catalyzed by artificial agents with an increasing dissociation rate constant K2. The catalytic role of several artificial agents in DNA self-assembly was verified by real-time fluorescent detection or agarose gel electrophoresis. We found that 20% PEG 200 could significantly catalyze DNA self-assembly and increase the reaction efficiency, such as linear hybridization chain reaction (HCR) and exponential hairpin assembly (EHA). Therefore, we foresee that a fast and efficient DNA self-assembly in structural DNA nanotechnology will be desirable.Chao ShiYifan WangMenghua ZhangCuiping MaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-5 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chao Shi Yifan Wang Menghua Zhang Cuiping Ma DNA Self-assembly Catalyzed by Artificial Agents
description	Abstract Nucleic acids have been shown to be versatile molecules and engineered to produce various nanostructures. However, the poor rate of these uncatalyzed nucleic acid reactions has restricted the development and applications. Herein, we reported a novel finding that DNA self-assembly could be nonenzymatically catalyzed by artificial agents with an increasing dissociation rate constant K2. The catalytic role of several artificial agents in DNA self-assembly was verified by real-time fluorescent detection or agarose gel electrophoresis. We found that 20% PEG 200 could significantly catalyze DNA self-assembly and increase the reaction efficiency, such as linear hybridization chain reaction (HCR) and exponential hairpin assembly (EHA). Therefore, we foresee that a fast and efficient DNA self-assembly in structural DNA nanotechnology will be desirable.
format	article
author	Chao Shi Yifan Wang Menghua Zhang Cuiping Ma
author_facet	Chao Shi Yifan Wang Menghua Zhang Cuiping Ma
author_sort	Chao Shi
title	DNA Self-assembly Catalyzed by Artificial Agents
title_short	DNA Self-assembly Catalyzed by Artificial Agents
title_full	DNA Self-assembly Catalyzed by Artificial Agents
title_fullStr	DNA Self-assembly Catalyzed by Artificial Agents
title_full_unstemmed	DNA Self-assembly Catalyzed by Artificial Agents
title_sort	dna self-assembly catalyzed by artificial agents
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/6ca7fd9b0a054bc7a9610cd2b750a439
work_keys_str_mv	AT chaoshi dnaselfassemblycatalyzedbyartificialagents AT yifanwang dnaselfassemblycatalyzedbyartificialagents AT menghuazhang dnaselfassemblycatalyzedbyartificialagents AT cuipingma dnaselfassemblycatalyzedbyartificialagents
_version_	1718395521025966080

DNA Self-assembly Catalyzed by Artificial Agents

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