Identification and validation of reference genes for transcript normalization in strawberry (Fragaria × ananassa) defense responses.

Strawberry (Fragaria spp) is an emerging model for the development of basic genomics and recombinant DNA studies among rosaceous crops. Functional genomic and molecular studies involve relative quantification of gene expression under experimental conditions of interest. Accuracy and reliability are...

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Autores principales:	Francisco Amil-Ruiz, José Garrido-Gala, Rosario Blanco-Portales, Kevin M Folta, Juan Muñoz-Blanco, José L Caballero
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6ca8e7bb74b0413db9b368c6bc437d2f
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spelling	oai:doaj.org-article:6ca8e7bb74b0413db9b368c6bc437d2f2021-11-18T09:01:19ZIdentification and validation of reference genes for transcript normalization in strawberry (Fragaria × ananassa) defense responses.1932-620310.1371/journal.pone.0070603https://doaj.org/article/6ca8e7bb74b0413db9b368c6bc437d2f2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23940602/?tool=EBIhttps://doaj.org/toc/1932-6203Strawberry (Fragaria spp) is an emerging model for the development of basic genomics and recombinant DNA studies among rosaceous crops. Functional genomic and molecular studies involve relative quantification of gene expression under experimental conditions of interest. Accuracy and reliability are dependent upon the choice of an optimal reference control transcript. There is no information available on validated endogenous reference genes for use in studies testing strawberry-pathogen interactions. Thirteen potential pre-selected strawberry reference genes were tested against different tissues, strawberry cultivars, biotic stresses, ripening and senescent conditions, and SA/JA treatments. Evaluation of reference candidate's suitability was analyzed by five different methodologies, and information was merged to identify best reference transcripts. A combination of all five methods was used for selective classification of reference genes. The resulting superior reference genes, FaRIB413, FaACTIN, FaEF1α and FaGAPDH2 are strongly recommended as control genes for relative quantification of gene expression in strawberry. This report constitutes the first systematic study to identify and validate optimal reference genes for accurate normalization of gene expression in strawberry plant defense response studies.Francisco Amil-RuizJosé Garrido-GalaRosario Blanco-PortalesKevin M FoltaJuan Muñoz-BlancoJosé L CaballeroPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 8, p e70603 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Francisco Amil-Ruiz José Garrido-Gala Rosario Blanco-Portales Kevin M Folta Juan Muñoz-Blanco José L Caballero Identification and validation of reference genes for transcript normalization in strawberry (Fragaria × ananassa) defense responses.
description	Strawberry (Fragaria spp) is an emerging model for the development of basic genomics and recombinant DNA studies among rosaceous crops. Functional genomic and molecular studies involve relative quantification of gene expression under experimental conditions of interest. Accuracy and reliability are dependent upon the choice of an optimal reference control transcript. There is no information available on validated endogenous reference genes for use in studies testing strawberry-pathogen interactions. Thirteen potential pre-selected strawberry reference genes were tested against different tissues, strawberry cultivars, biotic stresses, ripening and senescent conditions, and SA/JA treatments. Evaluation of reference candidate's suitability was analyzed by five different methodologies, and information was merged to identify best reference transcripts. A combination of all five methods was used for selective classification of reference genes. The resulting superior reference genes, FaRIB413, FaACTIN, FaEF1α and FaGAPDH2 are strongly recommended as control genes for relative quantification of gene expression in strawberry. This report constitutes the first systematic study to identify and validate optimal reference genes for accurate normalization of gene expression in strawberry plant defense response studies.
format	article
author	Francisco Amil-Ruiz José Garrido-Gala Rosario Blanco-Portales Kevin M Folta Juan Muñoz-Blanco José L Caballero
author_facet	Francisco Amil-Ruiz José Garrido-Gala Rosario Blanco-Portales Kevin M Folta Juan Muñoz-Blanco José L Caballero
author_sort	Francisco Amil-Ruiz
title	Identification and validation of reference genes for transcript normalization in strawberry (Fragaria × ananassa) defense responses.
title_short	Identification and validation of reference genes for transcript normalization in strawberry (Fragaria × ananassa) defense responses.
title_full	Identification and validation of reference genes for transcript normalization in strawberry (Fragaria × ananassa) defense responses.
title_fullStr	Identification and validation of reference genes for transcript normalization in strawberry (Fragaria × ananassa) defense responses.
title_full_unstemmed	Identification and validation of reference genes for transcript normalization in strawberry (Fragaria × ananassa) defense responses.
title_sort	identification and validation of reference genes for transcript normalization in strawberry (fragaria × ananassa) defense responses.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/6ca8e7bb74b0413db9b368c6bc437d2f
work_keys_str_mv	AT franciscoamilruiz identificationandvalidationofreferencegenesfortranscriptnormalizationinstrawberryfragariaananassadefenseresponses AT josegarridogala identificationandvalidationofreferencegenesfortranscriptnormalizationinstrawberryfragariaananassadefenseresponses AT rosarioblancoportales identificationandvalidationofreferencegenesfortranscriptnormalizationinstrawberryfragariaananassadefenseresponses AT kevinmfolta identificationandvalidationofreferencegenesfortranscriptnormalizationinstrawberryfragariaananassadefenseresponses AT juanmunozblanco identificationandvalidationofreferencegenesfortranscriptnormalizationinstrawberryfragariaananassadefenseresponses AT joselcaballero identificationandvalidationofreferencegenesfortranscriptnormalizationinstrawberryfragariaananassadefenseresponses
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Identification and validation of reference genes for transcript normalization in strawberry (Fragaria × ananassa) defense responses.

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