Subwavelength hyperspectral THz studies of articular cartilage

Abstract Terahertz-spectroscopy probes dynamics and spectral response of collective vibrational modes in condensed phase, which can yield insight into composition and topology. However, due to the long wavelengths employed (λ = 300 μm at 1THz), diffraction limited imaging is typically restricted to...

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Autores principales:	Rayko I. Stantchev, Jessica C. Mansfield, Ryan S. Edginton, Peter Hobson, Francesca Palombo, Euan Hendry
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6caed23553e54666851982fdc07b0268
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spelling	oai:doaj.org-article:6caed23553e54666851982fdc07b02682021-12-02T12:33:01ZSubwavelength hyperspectral THz studies of articular cartilage10.1038/s41598-018-25057-92045-2322https://doaj.org/article/6caed23553e54666851982fdc07b02682018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25057-9https://doaj.org/toc/2045-2322Abstract Terahertz-spectroscopy probes dynamics and spectral response of collective vibrational modes in condensed phase, which can yield insight into composition and topology. However, due to the long wavelengths employed (λ = 300 μm at 1THz), diffraction limited imaging is typically restricted to spatial resolutions around a millimeter. Here, we demonstrate a new form of subwavelength hyperspectral, polarization-resolved THz imaging which employs an optical pattern projected onto a 6 μm-thin silicon wafer to achieve near-field modulation of a co-incident THz pulse. By placing near-field scatterers, one can measure the interaction of object with the evanescent THz fields. Further, by measuring the temporal evolution of the THz field a sample’s permittivity can be extracted with 65 μm spatial resolution due to the presence of evanescent fields. Here, we present the first application of this new approach to articular cartilage. We show that the THz permittivity in this material varies progressively from the superficial zone to the deep layer, and that this correlates with a change in orientation of the collagen fibrils that compose the extracellular matrix (ECM) of the tissue. Our approach enables direct interrogation of the sample’s biophysical properties, in this case concerning the structure and permittivity of collagen fibrils and their anisotropic organisation in connective tissue.Rayko I. StantchevJessica C. MansfieldRyan S. EdgintonPeter HobsonFrancesca PalomboEuan HendryNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-8 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rayko I. Stantchev Jessica C. Mansfield Ryan S. Edginton Peter Hobson Francesca Palombo Euan Hendry Subwavelength hyperspectral THz studies of articular cartilage
description	Abstract Terahertz-spectroscopy probes dynamics and spectral response of collective vibrational modes in condensed phase, which can yield insight into composition and topology. However, due to the long wavelengths employed (λ = 300 μm at 1THz), diffraction limited imaging is typically restricted to spatial resolutions around a millimeter. Here, we demonstrate a new form of subwavelength hyperspectral, polarization-resolved THz imaging which employs an optical pattern projected onto a 6 μm-thin silicon wafer to achieve near-field modulation of a co-incident THz pulse. By placing near-field scatterers, one can measure the interaction of object with the evanescent THz fields. Further, by measuring the temporal evolution of the THz field a sample’s permittivity can be extracted with 65 μm spatial resolution due to the presence of evanescent fields. Here, we present the first application of this new approach to articular cartilage. We show that the THz permittivity in this material varies progressively from the superficial zone to the deep layer, and that this correlates with a change in orientation of the collagen fibrils that compose the extracellular matrix (ECM) of the tissue. Our approach enables direct interrogation of the sample’s biophysical properties, in this case concerning the structure and permittivity of collagen fibrils and their anisotropic organisation in connective tissue.
format	article
author	Rayko I. Stantchev Jessica C. Mansfield Ryan S. Edginton Peter Hobson Francesca Palombo Euan Hendry
author_facet	Rayko I. Stantchev Jessica C. Mansfield Ryan S. Edginton Peter Hobson Francesca Palombo Euan Hendry
author_sort	Rayko I. Stantchev
title	Subwavelength hyperspectral THz studies of articular cartilage
title_short	Subwavelength hyperspectral THz studies of articular cartilage
title_full	Subwavelength hyperspectral THz studies of articular cartilage
title_fullStr	Subwavelength hyperspectral THz studies of articular cartilage
title_full_unstemmed	Subwavelength hyperspectral THz studies of articular cartilage
title_sort	subwavelength hyperspectral thz studies of articular cartilage
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/6caed23553e54666851982fdc07b0268
work_keys_str_mv	AT raykoistantchev subwavelengthhyperspectralthzstudiesofarticularcartilage AT jessicacmansfield subwavelengthhyperspectralthzstudiesofarticularcartilage AT ryansedginton subwavelengthhyperspectralthzstudiesofarticularcartilage AT peterhobson subwavelengthhyperspectralthzstudiesofarticularcartilage AT francescapalombo subwavelengthhyperspectralthzstudiesofarticularcartilage AT euanhendry subwavelengthhyperspectralthzstudiesofarticularcartilage
_version_	1718393863131889664

Subwavelength hyperspectral THz studies of articular cartilage

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