Overexpression of BBX18 Promotes Thermomorphogenesis Through the PRR5-PIF4 Pathway

Thermomorphogenesis is the morphological response of plants to an elevation in the ambient temperature, which is mediated by the bHLH transcription factor PIF4. The evening-expressed clock component, PRR5, directly represses the expression of PIF4 mRNA. Additionally, PRR5 interacts with PIF4 protein...

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Autores principales:	Geonhee Hwang, Jeeyoon Park, Soohwan Kim, Jeonghyang Park, Dain Seo, Eunkyoo Oh
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	BBX18 PRR5 thermomorphogenesis PIF4 high temperature Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/6caf7fbd9d2643078d514513ace76ffb
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spelling	oai:doaj.org-article:6caf7fbd9d2643078d514513ace76ffb2021-11-30T16:38:24ZOverexpression of BBX18 Promotes Thermomorphogenesis Through the PRR5-PIF4 Pathway1664-462X10.3389/fpls.2021.782352https://doaj.org/article/6caf7fbd9d2643078d514513ace76ffb2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.782352/fullhttps://doaj.org/toc/1664-462XThermomorphogenesis is the morphological response of plants to an elevation in the ambient temperature, which is mediated by the bHLH transcription factor PIF4. The evening-expressed clock component, PRR5, directly represses the expression of PIF4 mRNA. Additionally, PRR5 interacts with PIF4 protein and represses its transactivation activity, which in turn suppresses the thermoresponsive growth in the evening. Here, we found that the B-box zinc finger protein, BBX18, interacts with PRR5 through the B-Box2 domain. Deletion of the B-Box2 domain abolished the functions of BBX18, including the stimulation of PIF4 mRNA expression and hypocotyl growth. Overexpression of BBX18, and not of B-Box2-deleted BBX18, restored the expression of thermoresponsive genes in the evening. We further show that BBX18 prevents PRR5 from inhibiting PIF4-mediated high temperature responses. Taken together, our results suggest that BBX18 regulates thermoresponsive growth through the PRR5-PIF4 pathway.Geonhee HwangJeeyoon ParkSoohwan KimJeonghyang ParkDain SeoEunkyoo OhFrontiers Media S.A.articleBBX18PRR5thermomorphogenesisPIF4high temperaturePlant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	BBX18 PRR5 thermomorphogenesis PIF4 high temperature Plant culture SB1-1110
spellingShingle	BBX18 PRR5 thermomorphogenesis PIF4 high temperature Plant culture SB1-1110 Geonhee Hwang Jeeyoon Park Soohwan Kim Jeonghyang Park Dain Seo Eunkyoo Oh Overexpression of BBX18 Promotes Thermomorphogenesis Through the PRR5-PIF4 Pathway
description	Thermomorphogenesis is the morphological response of plants to an elevation in the ambient temperature, which is mediated by the bHLH transcription factor PIF4. The evening-expressed clock component, PRR5, directly represses the expression of PIF4 mRNA. Additionally, PRR5 interacts with PIF4 protein and represses its transactivation activity, which in turn suppresses the thermoresponsive growth in the evening. Here, we found that the B-box zinc finger protein, BBX18, interacts with PRR5 through the B-Box2 domain. Deletion of the B-Box2 domain abolished the functions of BBX18, including the stimulation of PIF4 mRNA expression and hypocotyl growth. Overexpression of BBX18, and not of B-Box2-deleted BBX18, restored the expression of thermoresponsive genes in the evening. We further show that BBX18 prevents PRR5 from inhibiting PIF4-mediated high temperature responses. Taken together, our results suggest that BBX18 regulates thermoresponsive growth through the PRR5-PIF4 pathway.
format	article
author	Geonhee Hwang Jeeyoon Park Soohwan Kim Jeonghyang Park Dain Seo Eunkyoo Oh
author_facet	Geonhee Hwang Jeeyoon Park Soohwan Kim Jeonghyang Park Dain Seo Eunkyoo Oh
author_sort	Geonhee Hwang
title	Overexpression of BBX18 Promotes Thermomorphogenesis Through the PRR5-PIF4 Pathway
title_short	Overexpression of BBX18 Promotes Thermomorphogenesis Through the PRR5-PIF4 Pathway
title_full	Overexpression of BBX18 Promotes Thermomorphogenesis Through the PRR5-PIF4 Pathway
title_fullStr	Overexpression of BBX18 Promotes Thermomorphogenesis Through the PRR5-PIF4 Pathway
title_full_unstemmed	Overexpression of BBX18 Promotes Thermomorphogenesis Through the PRR5-PIF4 Pathway
title_sort	overexpression of bbx18 promotes thermomorphogenesis through the prr5-pif4 pathway
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/6caf7fbd9d2643078d514513ace76ffb
work_keys_str_mv	AT geonheehwang overexpressionofbbx18promotesthermomorphogenesisthroughtheprr5pif4pathway AT jeeyoonpark overexpressionofbbx18promotesthermomorphogenesisthroughtheprr5pif4pathway AT soohwankim overexpressionofbbx18promotesthermomorphogenesisthroughtheprr5pif4pathway AT jeonghyangpark overexpressionofbbx18promotesthermomorphogenesisthroughtheprr5pif4pathway AT dainseo overexpressionofbbx18promotesthermomorphogenesisthroughtheprr5pif4pathway AT eunkyoooh overexpressionofbbx18promotesthermomorphogenesisthroughtheprr5pif4pathway
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Overexpression of BBX18 Promotes Thermomorphogenesis Through the PRR5-PIF4 Pathway

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