Detection and Drug Susceptibility Testing of <i>Neisseria gonorrhoeae</i> Using Isothermal Microcalorimetry
Background: Gonorrhea is a frequently encountered sexually transmitted disease that results in urethritis and can further lead to pelvic inflammatory disease, infertility, and possibly disseminated gonococcal infections. Thus, it must be diagnosed promptly and accurately. In addition, drug susceptib...
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| Autores principales: | , , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/6cb08748f0d14ad6a073dde6de4eb04f |
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| Sumario: | Background: Gonorrhea is a frequently encountered sexually transmitted disease that results in urethritis and can further lead to pelvic inflammatory disease, infertility, and possibly disseminated gonococcal infections. Thus, it must be diagnosed promptly and accurately. In addition, drug susceptibility testing should be performed rapidly as well. Unfortunately, <i>Neisseria gonorrhoea</i> is a fastidious microorganism that is difficult to grow and requires culturing in an opaque medium. Methods: Here, we used isothermal microcalorimetry (IMC) to monitor the growth and the antimicrobial susceptibility of <i>N. gonorrhoea</i>. Results: Using IMC, concentrations of <i>N.</i> <i>gonorrhoea</i> between 2000 and 1 CFU·mL<sup>−1</sup> were detected within 12 to 33 h. In addition, drug susceptibility could be monitored easily. Conclusions: The use of isothermal microcalorimetry provides an interesting and useful tool to detect and characterize fastidious microbes such as <i>N. gonorrhoea</i> that require media incompatible with optical detection conventionally used in many commercial systems. |
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