Detection and Drug Susceptibility Testing of <i>Neisseria gonorrhoeae</i> Using Isothermal Microcalorimetry

Background: Gonorrhea is a frequently encountered sexually transmitted disease that results in urethritis and can further lead to pelvic inflammatory disease, infertility, and possibly disseminated gonococcal infections. Thus, it must be diagnosed promptly and accurately. In addition, drug susceptib...

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Autores principales:	Anabel E. Grütter, Tecla Lafranca, Aurelia Pahnita Sigg, Max Mariotti, Gernot Bonkat, Olivier Braissant
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	isothermal microcalorimetry <i>Neisseria gonorrhoea</i> antimicrobials Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/6cb08748f0d14ad6a073dde6de4eb04f
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spelling	oai:doaj.org-article:6cb08748f0d14ad6a073dde6de4eb04f2021-11-25T18:25:15ZDetection and Drug Susceptibility Testing of <i>Neisseria gonorrhoeae</i> Using Isothermal Microcalorimetry10.3390/microorganisms91123372076-2607https://doaj.org/article/6cb08748f0d14ad6a073dde6de4eb04f2021-11-01T00:00:00Zhttps://www.mdpi.com/2076-2607/9/11/2337https://doaj.org/toc/2076-2607Background: Gonorrhea is a frequently encountered sexually transmitted disease that results in urethritis and can further lead to pelvic inflammatory disease, infertility, and possibly disseminated gonococcal infections. Thus, it must be diagnosed promptly and accurately. In addition, drug susceptibility testing should be performed rapidly as well. Unfortunately, <i>Neisseria gonorrhoea</i> is a fastidious microorganism that is difficult to grow and requires culturing in an opaque medium. Methods: Here, we used isothermal microcalorimetry (IMC) to monitor the growth and the antimicrobial susceptibility of <i>N. gonorrhoea</i>. Results: Using IMC, concentrations of <i>N.</i> <i>gonorrhoea</i> between 2000 and 1 CFU·mL<sup>−1</sup> were detected within 12 to 33 h. In addition, drug susceptibility could be monitored easily. Conclusions: The use of isothermal microcalorimetry provides an interesting and useful tool to detect and characterize fastidious microbes such as <i>N. gonorrhoea</i> that require media incompatible with optical detection conventionally used in many commercial systems.Anabel E. GrütterTecla LafrancaAurelia Pahnita SiggMax MariottiGernot BonkatOlivier BraissantMDPI AGarticleisothermal microcalorimetry<i>Neisseria gonorrhoea</i>antimicrobialsBiology (General)QH301-705.5ENMicroorganisms, Vol 9, Iss 2337, p 2337 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	isothermal microcalorimetry <i>Neisseria gonorrhoea</i> antimicrobials Biology (General) QH301-705.5
spellingShingle	isothermal microcalorimetry <i>Neisseria gonorrhoea</i> antimicrobials Biology (General) QH301-705.5 Anabel E. Grütter Tecla Lafranca Aurelia Pahnita Sigg Max Mariotti Gernot Bonkat Olivier Braissant Detection and Drug Susceptibility Testing of <i>Neisseria gonorrhoeae</i> Using Isothermal Microcalorimetry
description	Background: Gonorrhea is a frequently encountered sexually transmitted disease that results in urethritis and can further lead to pelvic inflammatory disease, infertility, and possibly disseminated gonococcal infections. Thus, it must be diagnosed promptly and accurately. In addition, drug susceptibility testing should be performed rapidly as well. Unfortunately, <i>Neisseria gonorrhoea</i> is a fastidious microorganism that is difficult to grow and requires culturing in an opaque medium. Methods: Here, we used isothermal microcalorimetry (IMC) to monitor the growth and the antimicrobial susceptibility of <i>N. gonorrhoea</i>. Results: Using IMC, concentrations of <i>N.</i> <i>gonorrhoea</i> between 2000 and 1 CFU·mL<sup>−1</sup> were detected within 12 to 33 h. In addition, drug susceptibility could be monitored easily. Conclusions: The use of isothermal microcalorimetry provides an interesting and useful tool to detect and characterize fastidious microbes such as <i>N. gonorrhoea</i> that require media incompatible with optical detection conventionally used in many commercial systems.
format	article
author	Anabel E. Grütter Tecla Lafranca Aurelia Pahnita Sigg Max Mariotti Gernot Bonkat Olivier Braissant
author_facet	Anabel E. Grütter Tecla Lafranca Aurelia Pahnita Sigg Max Mariotti Gernot Bonkat Olivier Braissant
author_sort	Anabel E. Grütter
title	Detection and Drug Susceptibility Testing of <i>Neisseria gonorrhoeae</i> Using Isothermal Microcalorimetry
title_short	Detection and Drug Susceptibility Testing of <i>Neisseria gonorrhoeae</i> Using Isothermal Microcalorimetry
title_full	Detection and Drug Susceptibility Testing of <i>Neisseria gonorrhoeae</i> Using Isothermal Microcalorimetry
title_fullStr	Detection and Drug Susceptibility Testing of <i>Neisseria gonorrhoeae</i> Using Isothermal Microcalorimetry
title_full_unstemmed	Detection and Drug Susceptibility Testing of <i>Neisseria gonorrhoeae</i> Using Isothermal Microcalorimetry
title_sort	detection and drug susceptibility testing of <i>neisseria gonorrhoeae</i> using isothermal microcalorimetry
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/6cb08748f0d14ad6a073dde6de4eb04f
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Detection and Drug Susceptibility Testing of <i>Neisseria gonorrhoeae</i> Using Isothermal Microcalorimetry

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