Identification and characterization of bacterial biofilm production in clinical isolates of Staphylococcus spp. against antimicrobials

Justification and Objectives: Circulating blood is sterile and the presence of microorganisms can be of clinical interest, especially in the hospital environment, being able to cause infectious processes and substantially increase morbidity and mortality. The objective of this work was to characteri...

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Autores principales:	Lamartine Rodrigues Martins, Mariana Quitéria de Morais Silva, Carlos Alberto Medeiros Neto, Maria Izabelly Silva Pimentel, Igor Vasconcelos Rocha, Sibele Ribeiro de Oliveira
Formato:	article
Lenguaje:	PT
Publicado:	Universidade de Santa Cruz do Sul 2021
Materias:	biofilmes hemocultura staphylococcus Medicine R Internal medicine RC31-1245 Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/6cb24e06a9994f7d98034783b4a42dc2
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spelling	oai:doaj.org-article:6cb24e06a9994f7d98034783b4a42dc22021-11-11T13:45:28ZIdentification and characterization of bacterial biofilm production in clinical isolates of Staphylococcus spp. against antimicrobials2238-336010.17058/reci.v11i2.15303https://doaj.org/article/6cb24e06a9994f7d98034783b4a42dc22021-08-01T00:00:00Zhttps://online.unisc.br/seer/index.php/epidemiologia/article/view/15303https://doaj.org/toc/2238-3360Justification and Objectives: Circulating blood is sterile and the presence of microorganisms can be of clinical interest, especially in the hospital environment, being able to cause infectious processes and substantially increase morbidity and mortality. The objective of this work was to characterize the isolates of the genus Staphylococcus spp. from bloodstream infections as to the production of bacterial biofilm and resistance to the main antimicrobials used in clinical practice. Methods: Blood cultures were collected with an indication of positivity for bacterial growth from multiple sectors of the study hospital, which were subsequently processed to identify the bacterial genus through the use of phenotypic tests for Gram positive bacteria. The verification of the resistance profile was performed following the Kirby-Bauer disk diffusion. The identification of the production and quantification of the bacterial biofilm occurred following the protocol described by O’toole (2010). Results: The most frequent clinical isolate was Coagulase negative Staphylococci 38 (54.29%), followed by Staphylococcus aureus 32 (45.71%). Resistance to erythromycin, norfloxacin, levofloxacin and azithromycin was observed in most isolates (70%). Regarding methicillin, more MRSA (59.38%) than MR-CONS (47.37%) were isolated. The ICU was the place where the formation of the biofilm showed indicative data of greater adherence, which was associated with MRSA strains. Conclusion: The bacterial isolates associated with bloodstream infections showed high resistance to antimicrobials. The presence of MRSA and MR-CONS with strong and/or moderate biofilm production capacity represents a greater risk to the health of patients affected by infections caused by these agents.Lamartine Rodrigues MartinsMariana Quitéria de Morais SilvaCarlos Alberto Medeiros NetoMaria Izabelly Silva PimentelIgor Vasconcelos RochaSibele Ribeiro de OliveiraUniversidade de Santa Cruz do SularticlebiofilmeshemoculturastaphylococcusMedicineRInternal medicineRC31-1245Infectious and parasitic diseasesRC109-216PTRevista de Epidemiologia e Controle de Infecção, Vol 11, Iss 2 (2021)
institution	DOAJ
collection	DOAJ
language	PT
topic	biofilmes hemocultura staphylococcus Medicine R Internal medicine RC31-1245 Infectious and parasitic diseases RC109-216
spellingShingle	biofilmes hemocultura staphylococcus Medicine R Internal medicine RC31-1245 Infectious and parasitic diseases RC109-216 Lamartine Rodrigues Martins Mariana Quitéria de Morais Silva Carlos Alberto Medeiros Neto Maria Izabelly Silva Pimentel Igor Vasconcelos Rocha Sibele Ribeiro de Oliveira Identification and characterization of bacterial biofilm production in clinical isolates of Staphylococcus spp. against antimicrobials
description	Justification and Objectives: Circulating blood is sterile and the presence of microorganisms can be of clinical interest, especially in the hospital environment, being able to cause infectious processes and substantially increase morbidity and mortality. The objective of this work was to characterize the isolates of the genus Staphylococcus spp. from bloodstream infections as to the production of bacterial biofilm and resistance to the main antimicrobials used in clinical practice. Methods: Blood cultures were collected with an indication of positivity for bacterial growth from multiple sectors of the study hospital, which were subsequently processed to identify the bacterial genus through the use of phenotypic tests for Gram positive bacteria. The verification of the resistance profile was performed following the Kirby-Bauer disk diffusion. The identification of the production and quantification of the bacterial biofilm occurred following the protocol described by O’toole (2010). Results: The most frequent clinical isolate was Coagulase negative Staphylococci 38 (54.29%), followed by Staphylococcus aureus 32 (45.71%). Resistance to erythromycin, norfloxacin, levofloxacin and azithromycin was observed in most isolates (70%). Regarding methicillin, more MRSA (59.38%) than MR-CONS (47.37%) were isolated. The ICU was the place where the formation of the biofilm showed indicative data of greater adherence, which was associated with MRSA strains. Conclusion: The bacterial isolates associated with bloodstream infections showed high resistance to antimicrobials. The presence of MRSA and MR-CONS with strong and/or moderate biofilm production capacity represents a greater risk to the health of patients affected by infections caused by these agents.
format	article
author	Lamartine Rodrigues Martins Mariana Quitéria de Morais Silva Carlos Alberto Medeiros Neto Maria Izabelly Silva Pimentel Igor Vasconcelos Rocha Sibele Ribeiro de Oliveira
author_facet	Lamartine Rodrigues Martins Mariana Quitéria de Morais Silva Carlos Alberto Medeiros Neto Maria Izabelly Silva Pimentel Igor Vasconcelos Rocha Sibele Ribeiro de Oliveira
author_sort	Lamartine Rodrigues Martins
title	Identification and characterization of bacterial biofilm production in clinical isolates of Staphylococcus spp. against antimicrobials
title_short	Identification and characterization of bacterial biofilm production in clinical isolates of Staphylococcus spp. against antimicrobials
title_full	Identification and characterization of bacterial biofilm production in clinical isolates of Staphylococcus spp. against antimicrobials
title_fullStr	Identification and characterization of bacterial biofilm production in clinical isolates of Staphylococcus spp. against antimicrobials
title_full_unstemmed	Identification and characterization of bacterial biofilm production in clinical isolates of Staphylococcus spp. against antimicrobials
title_sort	identification and characterization of bacterial biofilm production in clinical isolates of staphylococcus spp. against antimicrobials
publisher	Universidade de Santa Cruz do Sul
publishDate	2021
url	https://doaj.org/article/6cb24e06a9994f7d98034783b4a42dc2
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Identification and characterization of bacterial biofilm production in clinical isolates of Staphylococcus spp. against antimicrobials

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