Flagella, Type I Fimbriae and Curli of Uropathogenic <i>Escherichia coli</i> Promote the Release of Proinflammatory Cytokines in a Coculture System
Background. Urinary tract infections (UTIs) are a public health problem in Mexico, and uropathogenic <i>Escherichia coli</i> (UPEC) is one of the main etiological agents. Flagella, type I fimbriae, and curli promote the ability of these bacteria to successfully colonize its host. Aim. Th...
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| Autores principales: | , , , , , , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/6cb349e5eae94f7ab11eca2e2191f369 |
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| Sumario: | Background. Urinary tract infections (UTIs) are a public health problem in Mexico, and uropathogenic <i>Escherichia coli</i> (UPEC) is one of the main etiological agents. Flagella, type I fimbriae, and curli promote the ability of these bacteria to successfully colonize its host. Aim. This study aimed to determine whether flagella-, type I fimbriae-, and curli-expressing UPEC induces the release of proinflammatory cytokines in an established coculture system. Methods. The <i>fliC</i>, <i>fimH</i>, and <i>csgA</i> genes by UPEC strain were disrupted by allelic replacement. Flagella, type I fimbriae, and curli were visualized by transmission electron microscopy (TEM). HTB-5 (upper chamber) and HMC-1 (lower chamber) cells cocultured in Transwell<sup>®</sup> plates were infected with these UPEC strains and purified proteins. There was adherence to HTB-5 cells treated with different UPEC strains and they were quantified as colony-forming units (CFU)/mL. Results. High concentrations of IL-6 and IL-8 were induced by the FimH and FliC proteins; however, these cytokines were detected in low concentrations in presence of CsgA. Compared with UPEC CFT073, CFT073Δ<i>fimH</i>, CFT073Δ<i>fimH</i>Δ<i>fliC</i>, and CFT073Δ<i>csgA</i>Δ<i>fimH</i> strains significantly reduced the adherence to HTB-5 cells. Conclusion. The FimH and FliC proteins are involved in IL-6 and IL-8 release in a coculture model of HTB-5 and HMC-1 cells. |
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