Flagella, Type I Fimbriae and Curli of Uropathogenic <i>Escherichia coli</i> Promote the Release of Proinflammatory Cytokines in a Coculture System

Background. Urinary tract infections (UTIs) are a public health problem in Mexico, and uropathogenic <i>Escherichia coli</i> (UPEC) is one of the main etiological agents. Flagella, type I fimbriae, and curli promote the ability of these bacteria to successfully colonize its host. Aim. Th...

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Autores principales:	Rubí Vega-Hernández, Sara A. Ochoa, Ricardo Valle-Rios, Gustavo A. Jaimes-Ortega, José Arellano-Galindo, Gerardo Aparicio-Ozores, José Antonio Ibarra, Rigoberto Hernández-Castro, Ariadnna Cruz-Córdova, Juan Xicohtencatl-Cortes
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	UPEC adherence fimbriae cytokines coculture Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/6cb349e5eae94f7ab11eca2e2191f369
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spelling	oai:doaj.org-article:6cb349e5eae94f7ab11eca2e2191f3692021-11-25T18:24:25ZFlagella, Type I Fimbriae and Curli of Uropathogenic <i>Escherichia coli</i> Promote the Release of Proinflammatory Cytokines in a Coculture System10.3390/microorganisms91122332076-2607https://doaj.org/article/6cb349e5eae94f7ab11eca2e2191f3692021-10-01T00:00:00Zhttps://www.mdpi.com/2076-2607/9/11/2233https://doaj.org/toc/2076-2607Background. Urinary tract infections (UTIs) are a public health problem in Mexico, and uropathogenic <i>Escherichia coli</i> (UPEC) is one of the main etiological agents. Flagella, type I fimbriae, and curli promote the ability of these bacteria to successfully colonize its host. Aim. This study aimed to determine whether flagella-, type I fimbriae-, and curli-expressing UPEC induces the release of proinflammatory cytokines in an established coculture system. Methods. The <i>fliC</i>, <i>fimH</i>, and <i>csgA</i> genes by UPEC strain were disrupted by allelic replacement. Flagella, type I fimbriae, and curli were visualized by transmission electron microscopy (TEM). HTB-5 (upper chamber) and HMC-1 (lower chamber) cells cocultured in Transwell<sup>®</sup> plates were infected with these UPEC strains and purified proteins. There was adherence to HTB-5 cells treated with different UPEC strains and they were quantified as colony-forming units (CFU)/mL. Results. High concentrations of IL-6 and IL-8 were induced by the FimH and FliC proteins; however, these cytokines were detected in low concentrations in presence of CsgA. Compared with UPEC CFT073, CFT073Δ<i>fimH</i>, CFT073Δ<i>fimH</i>Δ<i>fliC</i>, and CFT073Δ<i>csgA</i>Δ<i>fimH</i> strains significantly reduced the adherence to HTB-5 cells. Conclusion. The FimH and FliC proteins are involved in IL-6 and IL-8 release in a coculture model of HTB-5 and HMC-1 cells.Rubí Vega-HernándezSara A. OchoaRicardo Valle-RiosGustavo A. Jaimes-OrtegaJosé Arellano-GalindoGerardo Aparicio-OzoresJosé Antonio IbarraRigoberto Hernández-CastroAriadnna Cruz-CórdovaJuan Xicohtencatl-CortesMDPI AGarticleUPECadherencefimbriaecytokinescocultureBiology (General)QH301-705.5ENMicroorganisms, Vol 9, Iss 2233, p 2233 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	UPEC adherence fimbriae cytokines coculture Biology (General) QH301-705.5
spellingShingle	UPEC adherence fimbriae cytokines coculture Biology (General) QH301-705.5 Rubí Vega-Hernández Sara A. Ochoa Ricardo Valle-Rios Gustavo A. Jaimes-Ortega José Arellano-Galindo Gerardo Aparicio-Ozores José Antonio Ibarra Rigoberto Hernández-Castro Ariadnna Cruz-Córdova Juan Xicohtencatl-Cortes Flagella, Type I Fimbriae and Curli of Uropathogenic <i>Escherichia coli</i> Promote the Release of Proinflammatory Cytokines in a Coculture System
description	Background. Urinary tract infections (UTIs) are a public health problem in Mexico, and uropathogenic <i>Escherichia coli</i> (UPEC) is one of the main etiological agents. Flagella, type I fimbriae, and curli promote the ability of these bacteria to successfully colonize its host. Aim. This study aimed to determine whether flagella-, type I fimbriae-, and curli-expressing UPEC induces the release of proinflammatory cytokines in an established coculture system. Methods. The <i>fliC</i>, <i>fimH</i>, and <i>csgA</i> genes by UPEC strain were disrupted by allelic replacement. Flagella, type I fimbriae, and curli were visualized by transmission electron microscopy (TEM). HTB-5 (upper chamber) and HMC-1 (lower chamber) cells cocultured in Transwell<sup>®</sup> plates were infected with these UPEC strains and purified proteins. There was adherence to HTB-5 cells treated with different UPEC strains and they were quantified as colony-forming units (CFU)/mL. Results. High concentrations of IL-6 and IL-8 were induced by the FimH and FliC proteins; however, these cytokines were detected in low concentrations in presence of CsgA. Compared with UPEC CFT073, CFT073Δ<i>fimH</i>, CFT073Δ<i>fimH</i>Δ<i>fliC</i>, and CFT073Δ<i>csgA</i>Δ<i>fimH</i> strains significantly reduced the adherence to HTB-5 cells. Conclusion. The FimH and FliC proteins are involved in IL-6 and IL-8 release in a coculture model of HTB-5 and HMC-1 cells.
format	article
author	Rubí Vega-Hernández Sara A. Ochoa Ricardo Valle-Rios Gustavo A. Jaimes-Ortega José Arellano-Galindo Gerardo Aparicio-Ozores José Antonio Ibarra Rigoberto Hernández-Castro Ariadnna Cruz-Córdova Juan Xicohtencatl-Cortes
author_facet	Rubí Vega-Hernández Sara A. Ochoa Ricardo Valle-Rios Gustavo A. Jaimes-Ortega José Arellano-Galindo Gerardo Aparicio-Ozores José Antonio Ibarra Rigoberto Hernández-Castro Ariadnna Cruz-Córdova Juan Xicohtencatl-Cortes
author_sort	Rubí Vega-Hernández
title	Flagella, Type I Fimbriae and Curli of Uropathogenic <i>Escherichia coli</i> Promote the Release of Proinflammatory Cytokines in a Coculture System
title_short	Flagella, Type I Fimbriae and Curli of Uropathogenic <i>Escherichia coli</i> Promote the Release of Proinflammatory Cytokines in a Coculture System
title_full	Flagella, Type I Fimbriae and Curli of Uropathogenic <i>Escherichia coli</i> Promote the Release of Proinflammatory Cytokines in a Coculture System
title_fullStr	Flagella, Type I Fimbriae and Curli of Uropathogenic <i>Escherichia coli</i> Promote the Release of Proinflammatory Cytokines in a Coculture System
title_full_unstemmed	Flagella, Type I Fimbriae and Curli of Uropathogenic <i>Escherichia coli</i> Promote the Release of Proinflammatory Cytokines in a Coculture System
title_sort	flagella, type i fimbriae and curli of uropathogenic <i>escherichia coli</i> promote the release of proinflammatory cytokines in a coculture system
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/6cb349e5eae94f7ab11eca2e2191f369
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Flagella, Type I Fimbriae and Curli of Uropathogenic <i>Escherichia coli</i> Promote the Release of Proinflammatory Cytokines in a Coculture System

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