TailTimer: A device for automating data collection in the rodent tail immersion assay.

The tail immersion assay is a widely used method for measuring acute thermal pain in a way which is quantifiable and reproducible. It is non-invasive and measures response to a stimulus that may be encountered by an animal in its natural environment. However, quantification of tail withdrawal latenc...

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Autores principales:	Mallory E Udell, Jie Ni, Angel Garcia Martinez, Megan K Mulligan, Eva E Redei, Hao Chen
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6cc2d7c2abff4901b7bf08d5ecfc723c
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spelling	oai:doaj.org-article:6cc2d7c2abff4901b7bf08d5ecfc723c2021-12-02T20:17:44ZTailTimer: A device for automating data collection in the rodent tail immersion assay.1932-620310.1371/journal.pone.0256264https://doaj.org/article/6cc2d7c2abff4901b7bf08d5ecfc723c2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0256264https://doaj.org/toc/1932-6203The tail immersion assay is a widely used method for measuring acute thermal pain in a way which is quantifiable and reproducible. It is non-invasive and measures response to a stimulus that may be encountered by an animal in its natural environment. However, quantification of tail withdrawal latency relies on manual timing of tail flick using a stopwatch, and precise temperatures of the water at the time of measurement are most often not recorded. These two factors greatly reduce the reproducibility of tail immersion assay data and likely contribute to some of the discrepancies present among relevant literature. We designed a device, TailTimer, which uses a Raspberry Pi single-board computer, a digital temperature sensor, and two electrical wires, to automatically record tail withdrawal latency and water temperature. We programmed TailTimer to continuously display and record water temperature and to only permit the assay to be conducted when the water is within ± 0.25°C of the target temperature. Our software also records the identification of the animals using a radio frequency identification (RFID) system. We further adapted the RFID system to recognize several specific keys as user interface commands, allowing TailTimer to be operated via RFID fobs for increased usability. Data recorded using the TailTimer device showed a negative linear relationship between tail withdrawal latency and water temperature when tested between 47-50°C. We also observed a previously unreported, yet profound, effect of water mixing speed on latency. In one experiment using TailTimer, we observed significantly longer latencies following administration of oral oxycodone versus a distilled water control when measured after 15 mins or 1 h, but not after 4 h. TailTimer also detected significant strain differences in baseline latency. These findings valorize TailTimer in its sensitivity and reliability for measuring thermal pain thresholds.Mallory E UdellJie NiAngel Garcia MartinezMegan K MulliganEva E RedeiHao ChenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 8, p e0256264 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mallory E Udell Jie Ni Angel Garcia Martinez Megan K Mulligan Eva E Redei Hao Chen TailTimer: A device for automating data collection in the rodent tail immersion assay.
description	The tail immersion assay is a widely used method for measuring acute thermal pain in a way which is quantifiable and reproducible. It is non-invasive and measures response to a stimulus that may be encountered by an animal in its natural environment. However, quantification of tail withdrawal latency relies on manual timing of tail flick using a stopwatch, and precise temperatures of the water at the time of measurement are most often not recorded. These two factors greatly reduce the reproducibility of tail immersion assay data and likely contribute to some of the discrepancies present among relevant literature. We designed a device, TailTimer, which uses a Raspberry Pi single-board computer, a digital temperature sensor, and two electrical wires, to automatically record tail withdrawal latency and water temperature. We programmed TailTimer to continuously display and record water temperature and to only permit the assay to be conducted when the water is within ± 0.25°C of the target temperature. Our software also records the identification of the animals using a radio frequency identification (RFID) system. We further adapted the RFID system to recognize several specific keys as user interface commands, allowing TailTimer to be operated via RFID fobs for increased usability. Data recorded using the TailTimer device showed a negative linear relationship between tail withdrawal latency and water temperature when tested between 47-50°C. We also observed a previously unreported, yet profound, effect of water mixing speed on latency. In one experiment using TailTimer, we observed significantly longer latencies following administration of oral oxycodone versus a distilled water control when measured after 15 mins or 1 h, but not after 4 h. TailTimer also detected significant strain differences in baseline latency. These findings valorize TailTimer in its sensitivity and reliability for measuring thermal pain thresholds.
format	article
author	Mallory E Udell Jie Ni Angel Garcia Martinez Megan K Mulligan Eva E Redei Hao Chen
author_facet	Mallory E Udell Jie Ni Angel Garcia Martinez Megan K Mulligan Eva E Redei Hao Chen
author_sort	Mallory E Udell
title	TailTimer: A device for automating data collection in the rodent tail immersion assay.
title_short	TailTimer: A device for automating data collection in the rodent tail immersion assay.
title_full	TailTimer: A device for automating data collection in the rodent tail immersion assay.
title_fullStr	TailTimer: A device for automating data collection in the rodent tail immersion assay.
title_full_unstemmed	TailTimer: A device for automating data collection in the rodent tail immersion assay.
title_sort	tailtimer: a device for automating data collection in the rodent tail immersion assay.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/6cc2d7c2abff4901b7bf08d5ecfc723c
work_keys_str_mv	AT malloryeudell tailtimeradeviceforautomatingdatacollectionintherodenttailimmersionassay AT jieni tailtimeradeviceforautomatingdatacollectionintherodenttailimmersionassay AT angelgarciamartinez tailtimeradeviceforautomatingdatacollectionintherodenttailimmersionassay AT megankmulligan tailtimeradeviceforautomatingdatacollectionintherodenttailimmersionassay AT evaeredei tailtimeradeviceforautomatingdatacollectionintherodenttailimmersionassay AT haochen tailtimeradeviceforautomatingdatacollectionintherodenttailimmersionassay
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TailTimer: A device for automating data collection in the rodent tail immersion assay.

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