Protective roles of grass carp Ctenopharyngodon idella Mx isoforms against grass carp reovirus.

<h4>Background</h4>Myxovirus resistance (Mx) proteins are crucial effectors of the innate antiviral response against a wide range of viruses, mediated by the type I interferon (IFN-I) signaling pathway. However, the antiviral activity of Mx proteins is diverse and complicated in differen...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Limin Peng, Chunrong Yang, Jianguo Su
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6cd5e09f4ed341c79e9b98b31068782b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:6cd5e09f4ed341c79e9b98b31068782b
record_format	dspace
spelling	oai:doaj.org-article:6cd5e09f4ed341c79e9b98b31068782b2021-11-18T08:05:01ZProtective roles of grass carp Ctenopharyngodon idella Mx isoforms against grass carp reovirus.1932-620310.1371/journal.pone.0052142https://doaj.org/article/6cd5e09f4ed341c79e9b98b31068782b2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23251697/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Myxovirus resistance (Mx) proteins are crucial effectors of the innate antiviral response against a wide range of viruses, mediated by the type I interferon (IFN-I) signaling pathway. However, the antiviral activity of Mx proteins is diverse and complicated in different species.<h4>Methodology/principal findings</h4>In the current study, two novel Mx genes (CiMx1 and CiMx3) were identified in grass carp (Ctenopharyngodon idella). CiMx1 and CiMx3 proteins exhibit high sequence identity (92.1%), and low identity with CiMx2 (49.2% and 49.5%, respectively) from the GenBank database. The predicted three-dimensional (3D) structures are distinct among the three isoforms. mRNA instability motifs also display significant differences in the three genes. The spatial and temporal expression profiles of three C. idella Mx genes and the IFN-I gene were investigated by real-time fluorescence quantitative RT-PCR (qRT-PCR) following infection with grass carp reovirus (GCRV) in vivo and in vitro. The results demonstrated that all the four genes were implicated in the anti-GCRV immune response, that mRNA expression of Mx genes might be independent of IFN-I, and that CIK cells are suitable for antiviral studies. By comparing expression patterns following GCRV challenge or poly(I:C) treatment, it was observed that GCRV blocks mRNA expression of the four genes. To determine the functions of Mx genes, three CiMx cDNAs were cloned into expression vectors and utilized for transfection of CIK cells. The protection conferred by each recombinant CiMx protein against GCRV infection was evaluated. Antiviral activity against GCRV was demonstrated by reduced cytopathic effect, lower virus titer and lower levels of expressed viral transcripts. The transcription of IFN-I gene was also monitored.<h4>Conclusions/significance</h4>The results indicate all three Mx genes can suppress replication of grass carp reovirus and over-expression of Mx genes mediate feedback inhibition of the IFN-I gene.Limin PengChunrong YangJianguo SuJianguo SuPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 12, p e52142 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Limin Peng Chunrong Yang Jianguo Su Jianguo Su Protective roles of grass carp Ctenopharyngodon idella Mx isoforms against grass carp reovirus.
description	<h4>Background</h4>Myxovirus resistance (Mx) proteins are crucial effectors of the innate antiviral response against a wide range of viruses, mediated by the type I interferon (IFN-I) signaling pathway. However, the antiviral activity of Mx proteins is diverse and complicated in different species.<h4>Methodology/principal findings</h4>In the current study, two novel Mx genes (CiMx1 and CiMx3) were identified in grass carp (Ctenopharyngodon idella). CiMx1 and CiMx3 proteins exhibit high sequence identity (92.1%), and low identity with CiMx2 (49.2% and 49.5%, respectively) from the GenBank database. The predicted three-dimensional (3D) structures are distinct among the three isoforms. mRNA instability motifs also display significant differences in the three genes. The spatial and temporal expression profiles of three C. idella Mx genes and the IFN-I gene were investigated by real-time fluorescence quantitative RT-PCR (qRT-PCR) following infection with grass carp reovirus (GCRV) in vivo and in vitro. The results demonstrated that all the four genes were implicated in the anti-GCRV immune response, that mRNA expression of Mx genes might be independent of IFN-I, and that CIK cells are suitable for antiviral studies. By comparing expression patterns following GCRV challenge or poly(I:C) treatment, it was observed that GCRV blocks mRNA expression of the four genes. To determine the functions of Mx genes, three CiMx cDNAs were cloned into expression vectors and utilized for transfection of CIK cells. The protection conferred by each recombinant CiMx protein against GCRV infection was evaluated. Antiviral activity against GCRV was demonstrated by reduced cytopathic effect, lower virus titer and lower levels of expressed viral transcripts. The transcription of IFN-I gene was also monitored.<h4>Conclusions/significance</h4>The results indicate all three Mx genes can suppress replication of grass carp reovirus and over-expression of Mx genes mediate feedback inhibition of the IFN-I gene.
format	article
author	Limin Peng Chunrong Yang Jianguo Su Jianguo Su
author_facet	Limin Peng Chunrong Yang Jianguo Su Jianguo Su
author_sort	Limin Peng
title	Protective roles of grass carp Ctenopharyngodon idella Mx isoforms against grass carp reovirus.
title_short	Protective roles of grass carp Ctenopharyngodon idella Mx isoforms against grass carp reovirus.
title_full	Protective roles of grass carp Ctenopharyngodon idella Mx isoforms against grass carp reovirus.
title_fullStr	Protective roles of grass carp Ctenopharyngodon idella Mx isoforms against grass carp reovirus.
title_full_unstemmed	Protective roles of grass carp Ctenopharyngodon idella Mx isoforms against grass carp reovirus.
title_sort	protective roles of grass carp ctenopharyngodon idella mx isoforms against grass carp reovirus.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/6cd5e09f4ed341c79e9b98b31068782b
work_keys_str_mv	AT liminpeng protectiverolesofgrasscarpctenopharyngodonidellamxisoformsagainstgrasscarpreovirus AT chunrongyang protectiverolesofgrasscarpctenopharyngodonidellamxisoformsagainstgrasscarpreovirus AT jianguosu protectiverolesofgrasscarpctenopharyngodonidellamxisoformsagainstgrasscarpreovirus AT jianguosu protectiverolesofgrasscarpctenopharyngodonidellamxisoformsagainstgrasscarpreovirus
_version_	1718422233597083648

Protective roles of grass carp Ctenopharyngodon idella Mx isoforms against grass carp reovirus.

Ejemplares similares