Reduction-responsive cross-linked stearyl peptide for effective delivery of plasmid DNA

Chong Yao,1,* Zongguang Tai,1,* Xiaoyu Wang,1,* Jiyong Liu,1 Quangang Zhu,1,2 Xin Wu,3 Lijuan Zhang,1 Wei Zhang,4 Jing Tian,1 Yuan Gao,1 Shen Gao1 1Department of Pharmaceutics, Changhai Hospital, Second Military Medical University, 2Department of Pharmacy, Yueyang Hospital of Integrated Traditional...

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Autores principales:	Yao C, Tai Z, Wang X, Liu J, Zhu Q, Wu X, Zhang L, Zhang W, Tian J, Gao Y, Gao S
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/6cdae9010fd140a68a6ef2ce644fc88e
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spelling	oai:doaj.org-article:6cdae9010fd140a68a6ef2ce644fc88e2021-12-02T05:03:00ZReduction-responsive cross-linked stearyl peptide for effective delivery of plasmid DNA1178-2013https://doaj.org/article/6cdae9010fd140a68a6ef2ce644fc88e2015-05-01T00:00:00Zhttp://www.dovepress.com/reduction-responsive-cross-linked-stearyl-peptide-for-effective-delive-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Chong Yao,1,* Zongguang Tai,1,* Xiaoyu Wang,1,* Jiyong Liu,1 Quangang Zhu,1,2 Xin Wu,3 Lijuan Zhang,1 Wei Zhang,4 Jing Tian,1 Yuan Gao,1 Shen Gao1 1Department of Pharmaceutics, Changhai Hospital, Second Military Medical University, 2Department of Pharmacy, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, 3Department of Pharmaceutics, Shanghai First People’s Hospital, Shanghai Jiaotong University School of Medicine, 4Department of Pharmaceutics, Shanghai Pulmonary Hospital, Shanghai Tongji University, Shanghai, People’s Republic of China *These authors contributed equally to this work Abstract: Low efficiency and significant toxicity are the main obstacles to successful gene delivery. We have developed a cationic reduction-responsive vector based on a disulfide cross-linked stearylated polyarginine peptide modified with histidine (C-SHR) for DNA delivery. The structure of the C-SHR was characterized, and the in vitro and in vivo transfection efficiency and cytotoxicity of C-SHR/plasmid DNA complexes were examined. Compared with non-cross-linked stearylated polyarginine peptide (SHR), C-SHR increased the intracellular uptake and dissociation behavior of the complexes. In addition, the gene transfection efficiency of C-SHR/plasmid DNA complexes in HEK293 and HeLa cells was improved and was comparable with that of bPEI-25K/plasmid DNA complexes, and the cytotoxicity of C-SHR was significantly less than that of bPEI-25K. Importantly, the in vivo gene transfection efficiency of C-SHR/plasmid DNA complexes was five fold higher than that of SHR/plasmid DNA complexes, suggesting that C-SHR is an efficient non-viral vector for DNA delivery.Keywords: polyarginine, histidine, stearyl, reduction-responsive, peptide, DNA deliveryYao CTai ZWang XLiu JZhu QWu XZhang LZhang WTian JGao YGao SDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 3403-3416 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Yao C Tai Z Wang X Liu J Zhu Q Wu X Zhang L Zhang W Tian J Gao Y Gao S Reduction-responsive cross-linked stearyl peptide for effective delivery of plasmid DNA
description	Chong Yao,1,* Zongguang Tai,1,* Xiaoyu Wang,1,* Jiyong Liu,1 Quangang Zhu,1,2 Xin Wu,3 Lijuan Zhang,1 Wei Zhang,4 Jing Tian,1 Yuan Gao,1 Shen Gao1 1Department of Pharmaceutics, Changhai Hospital, Second Military Medical University, 2Department of Pharmacy, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, 3Department of Pharmaceutics, Shanghai First People’s Hospital, Shanghai Jiaotong University School of Medicine, 4Department of Pharmaceutics, Shanghai Pulmonary Hospital, Shanghai Tongji University, Shanghai, People’s Republic of China *These authors contributed equally to this work Abstract: Low efficiency and significant toxicity are the main obstacles to successful gene delivery. We have developed a cationic reduction-responsive vector based on a disulfide cross-linked stearylated polyarginine peptide modified with histidine (C-SHR) for DNA delivery. The structure of the C-SHR was characterized, and the in vitro and in vivo transfection efficiency and cytotoxicity of C-SHR/plasmid DNA complexes were examined. Compared with non-cross-linked stearylated polyarginine peptide (SHR), C-SHR increased the intracellular uptake and dissociation behavior of the complexes. In addition, the gene transfection efficiency of C-SHR/plasmid DNA complexes in HEK293 and HeLa cells was improved and was comparable with that of bPEI-25K/plasmid DNA complexes, and the cytotoxicity of C-SHR was significantly less than that of bPEI-25K. Importantly, the in vivo gene transfection efficiency of C-SHR/plasmid DNA complexes was five fold higher than that of SHR/plasmid DNA complexes, suggesting that C-SHR is an efficient non-viral vector for DNA delivery.Keywords: polyarginine, histidine, stearyl, reduction-responsive, peptide, DNA delivery
format	article
author	Yao C Tai Z Wang X Liu J Zhu Q Wu X Zhang L Zhang W Tian J Gao Y Gao S
author_facet	Yao C Tai Z Wang X Liu J Zhu Q Wu X Zhang L Zhang W Tian J Gao Y Gao S
author_sort	Yao C
title	Reduction-responsive cross-linked stearyl peptide for effective delivery of plasmid DNA
title_short	Reduction-responsive cross-linked stearyl peptide for effective delivery of plasmid DNA
title_full	Reduction-responsive cross-linked stearyl peptide for effective delivery of plasmid DNA
title_fullStr	Reduction-responsive cross-linked stearyl peptide for effective delivery of plasmid DNA
title_full_unstemmed	Reduction-responsive cross-linked stearyl peptide for effective delivery of plasmid DNA
title_sort	reduction-responsive cross-linked stearyl peptide for effective delivery of plasmid dna
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/6cdae9010fd140a68a6ef2ce644fc88e
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Reduction-responsive cross-linked stearyl peptide for effective delivery of plasmid DNA

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