Arginine Induced Streptococcus gordonii Biofilm Detachment Using a Novel Rotating-Disc Rheometry Method

Oral diseases are one of the most common pathologies affecting human health. These diseases are typically associated with dental plaque-biofilms, through either build-up of the biofilm or dysbiosis of the microbial community. Arginine can disrupt dental plaque-biofilms, and maintain plaque homeostas...

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Autores principales:	Erin S. Gloag, Daniel J. Wozniak, Kevin L. Wolf, James G. Masters, Carlo Amorin Daep, Paul Stoodley
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	viscoelasticity biophysical mechanics Streptococcus gordonii arginine dental plaque Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/6ce74103428041478892d64baa1f2e82
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spelling	oai:doaj.org-article:6ce74103428041478892d64baa1f2e822021-11-05T14:44:54ZArginine Induced Streptococcus gordonii Biofilm Detachment Using a Novel Rotating-Disc Rheometry Method2235-298810.3389/fcimb.2021.784388https://doaj.org/article/6ce74103428041478892d64baa1f2e822021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fcimb.2021.784388/fullhttps://doaj.org/toc/2235-2988Oral diseases are one of the most common pathologies affecting human health. These diseases are typically associated with dental plaque-biofilms, through either build-up of the biofilm or dysbiosis of the microbial community. Arginine can disrupt dental plaque-biofilms, and maintain plaque homeostasis, making it an ideal therapeutic to combat the development of oral disease. Despite our understanding of the actions of arginine towards dental plaque-biofilms, it is still unclear how or if arginine effects the mechanical integrity of the dental plaque-biofilm. Here we adapted a rotating-disc rheometry assay, a method used to quantify marine biofilm fouling, to study how arginine treatment of Streptococcus gordonii biofilms influences biofilm detachment from surfaces. We demonstrate that the assay is highly sensitive at quantifying the presence of biofilm and the detachment or rearrangement of the biofilm structure as a function of shear stress. We demonstrate that arginine treatment leads to earlier detachment of the biofilm, indicating that arginine treatment weakens the biofilm, making it more susceptible to removal by shear stresses. Finally, we demonstrate that the biofilm disrupting affect is specific to arginine, and not a general property of amino acids, as S. gordonii biofilms treated with either glycine or lysine had mechanical properties similar to untreated biofilms. Our results add to the understanding that arginine targets biofilms by multifaceted mechanisms, both metabolic and physical, further promoting the potential of arginine as an active compound in dentifrices to maintain oral health.Erin S. GloagDaniel J. WozniakDaniel J. WozniakKevin L. WolfJames G. MastersCarlo Amorin DaepPaul StoodleyPaul StoodleyPaul StoodleyFrontiers Media S.A.articleviscoelasticitybiophysicalmechanicsStreptococcus gordoniiargininedental plaqueMicrobiologyQR1-502ENFrontiers in Cellular and Infection Microbiology, Vol 11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	viscoelasticity biophysical mechanics Streptococcus gordonii arginine dental plaque Microbiology QR1-502
spellingShingle	viscoelasticity biophysical mechanics Streptococcus gordonii arginine dental plaque Microbiology QR1-502 Erin S. Gloag Daniel J. Wozniak Daniel J. Wozniak Kevin L. Wolf James G. Masters Carlo Amorin Daep Paul Stoodley Paul Stoodley Paul Stoodley Arginine Induced Streptococcus gordonii Biofilm Detachment Using a Novel Rotating-Disc Rheometry Method
description	Oral diseases are one of the most common pathologies affecting human health. These diseases are typically associated with dental plaque-biofilms, through either build-up of the biofilm or dysbiosis of the microbial community. Arginine can disrupt dental plaque-biofilms, and maintain plaque homeostasis, making it an ideal therapeutic to combat the development of oral disease. Despite our understanding of the actions of arginine towards dental plaque-biofilms, it is still unclear how or if arginine effects the mechanical integrity of the dental plaque-biofilm. Here we adapted a rotating-disc rheometry assay, a method used to quantify marine biofilm fouling, to study how arginine treatment of Streptococcus gordonii biofilms influences biofilm detachment from surfaces. We demonstrate that the assay is highly sensitive at quantifying the presence of biofilm and the detachment or rearrangement of the biofilm structure as a function of shear stress. We demonstrate that arginine treatment leads to earlier detachment of the biofilm, indicating that arginine treatment weakens the biofilm, making it more susceptible to removal by shear stresses. Finally, we demonstrate that the biofilm disrupting affect is specific to arginine, and not a general property of amino acids, as S. gordonii biofilms treated with either glycine or lysine had mechanical properties similar to untreated biofilms. Our results add to the understanding that arginine targets biofilms by multifaceted mechanisms, both metabolic and physical, further promoting the potential of arginine as an active compound in dentifrices to maintain oral health.
format	article
author	Erin S. Gloag Daniel J. Wozniak Daniel J. Wozniak Kevin L. Wolf James G. Masters Carlo Amorin Daep Paul Stoodley Paul Stoodley Paul Stoodley
author_facet	Erin S. Gloag Daniel J. Wozniak Daniel J. Wozniak Kevin L. Wolf James G. Masters Carlo Amorin Daep Paul Stoodley Paul Stoodley Paul Stoodley
author_sort	Erin S. Gloag
title	Arginine Induced Streptococcus gordonii Biofilm Detachment Using a Novel Rotating-Disc Rheometry Method
title_short	Arginine Induced Streptococcus gordonii Biofilm Detachment Using a Novel Rotating-Disc Rheometry Method
title_full	Arginine Induced Streptococcus gordonii Biofilm Detachment Using a Novel Rotating-Disc Rheometry Method
title_fullStr	Arginine Induced Streptococcus gordonii Biofilm Detachment Using a Novel Rotating-Disc Rheometry Method
title_full_unstemmed	Arginine Induced Streptococcus gordonii Biofilm Detachment Using a Novel Rotating-Disc Rheometry Method
title_sort	arginine induced streptococcus gordonii biofilm detachment using a novel rotating-disc rheometry method
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/6ce74103428041478892d64baa1f2e82
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Arginine Induced Streptococcus gordonii Biofilm Detachment Using a Novel Rotating-Disc Rheometry Method

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