Influence of charge ratio of liposome/DNA complexes on their size after extrusion and transfection efficiency

Marija Brgles, Maja &Scaron;antak, Beata Halassy, Dubravko Forcic, Jelka Toma&scaron;icInstitute of Immunology, Research and Development Department, Zagreb, CroatiaBackground: Physicochemical characteristics of liposome/DNA complexes influence transfection efficiency and affect each...

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Autores principales:	Brgles M, &Scaron;antak M, Halassy B, Forcic D, Toma&scaron;ić J
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/6ce9aad57b234672a9fb475bf50a487b
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spelling	oai:doaj.org-article:6ce9aad57b234672a9fb475bf50a487b2021-12-02T02:16:49ZInfluence of charge ratio of liposome/DNA complexes on their size after extrusion and transfection efficiency1176-91141178-2013https://doaj.org/article/6ce9aad57b234672a9fb475bf50a487b2012-01-01T00:00:00Zhttp://www.dovepress.com/influence-of-charge-ratio-of-liposomedna-complexes-on-their-size-after-a9138https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Marija Brgles, Maja &Scaron;antak, Beata Halassy, Dubravko Forcic, Jelka Toma&scaron;icInstitute of Immunology, Research and Development Department, Zagreb, CroatiaBackground: Physicochemical characteristics of liposome/DNA complexes influence transfection efficiency and affect each other in a very intricate way. The result of this is discrepancies in conclusions drawn about the individual influence of each one.Methods: Aiming to elucidate the influence of liposome/DNA charge ratio and size on transfection efficiency and on each other, we used liposome/DNA complexes with charge ratio (+/-) in the range of 1&ndash;50 and extruded through membranes of 400, 200, and 100 nm. Plasmid DNA encoding green fluorescent protein was used to measure transfection efficiency by flow cytometry. Sizes of liposome/DNA complexes were measured by dynamic light scattering.Results: Liposome size was reduced after extrusion but this was mainly driven by the charge ratio and not by the size of the membrane pores. Reduction of complex size at each charge ratio positively correlated with transfection efficiency. When the size of the complexes was approximately constant, increasing the charge ratio was found to promote transfection efficiency. Cationic lipid N-(1-(2,3-dioleoyloxy)propyl)N,N,N trimethylammonium chloride was used for modulation of positive charge and a cytotoxicity test showed that increasing its amount increases cytotoxicity.Conclusion: It can be concluded that charge ratio dictates the size of the complex whereas overall size reduction and higher charge ratios promote transfection efficiency in vitro.Keywords: transfection efficiency, liposome charge, liposome sizeBrgles M&Scaron;antak MHalassy BForcic DToma&scaron;ić JDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 393-401 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Brgles M &Scaron;antak M Halassy B Forcic D Toma&scaron;ić J Influence of charge ratio of liposome/DNA complexes on their size after extrusion and transfection efficiency
description	Marija Brgles, Maja &Scaron;antak, Beata Halassy, Dubravko Forcic, Jelka Toma&scaron;icInstitute of Immunology, Research and Development Department, Zagreb, CroatiaBackground: Physicochemical characteristics of liposome/DNA complexes influence transfection efficiency and affect each other in a very intricate way. The result of this is discrepancies in conclusions drawn about the individual influence of each one.Methods: Aiming to elucidate the influence of liposome/DNA charge ratio and size on transfection efficiency and on each other, we used liposome/DNA complexes with charge ratio (+/-) in the range of 1&ndash;50 and extruded through membranes of 400, 200, and 100 nm. Plasmid DNA encoding green fluorescent protein was used to measure transfection efficiency by flow cytometry. Sizes of liposome/DNA complexes were measured by dynamic light scattering.Results: Liposome size was reduced after extrusion but this was mainly driven by the charge ratio and not by the size of the membrane pores. Reduction of complex size at each charge ratio positively correlated with transfection efficiency. When the size of the complexes was approximately constant, increasing the charge ratio was found to promote transfection efficiency. Cationic lipid N-(1-(2,3-dioleoyloxy)propyl)N,N,N trimethylammonium chloride was used for modulation of positive charge and a cytotoxicity test showed that increasing its amount increases cytotoxicity.Conclusion: It can be concluded that charge ratio dictates the size of the complex whereas overall size reduction and higher charge ratios promote transfection efficiency in vitro.Keywords: transfection efficiency, liposome charge, liposome size
format	article
author	Brgles M &Scaron;antak M Halassy B Forcic D Toma&scaron;ić J
author_facet	Brgles M &Scaron;antak M Halassy B Forcic D Toma&scaron;ić J
author_sort	Brgles M
title	Influence of charge ratio of liposome/DNA complexes on their size after extrusion and transfection efficiency
title_short	Influence of charge ratio of liposome/DNA complexes on their size after extrusion and transfection efficiency
title_full	Influence of charge ratio of liposome/DNA complexes on their size after extrusion and transfection efficiency
title_fullStr	Influence of charge ratio of liposome/DNA complexes on their size after extrusion and transfection efficiency
title_full_unstemmed	Influence of charge ratio of liposome/DNA complexes on their size after extrusion and transfection efficiency
title_sort	influence of charge ratio of liposome/dna complexes on their size after extrusion and transfection efficiency
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/6ce9aad57b234672a9fb475bf50a487b
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Influence of charge ratio of liposome/DNA complexes on their size after extrusion and transfection efficiency

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