Highly sensitive detection of dengue biomarker using streptavidin-conjugated quantum dots

Abstract A highly sensitive immunosensor using streptavidin-conjugated quantum dots (QDs/SA) was developed to detect dengue biomarker of non-structural protein 1 (NS1) at very low concentration, so that it can probe dengue infection even in the early stage. The QDs/SA were first bound to biotinylate...

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Autores principales:	Linh Tran, Sangkwon Park
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6cf34517d3d84b638e0193b3ca5c3842
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spelling	oai:doaj.org-article:6cf34517d3d84b638e0193b3ca5c38422021-12-02T16:30:10ZHighly sensitive detection of dengue biomarker using streptavidin-conjugated quantum dots10.1038/s41598-021-94172-x2045-2322https://doaj.org/article/6cf34517d3d84b638e0193b3ca5c38422021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94172-xhttps://doaj.org/toc/2045-2322Abstract A highly sensitive immunosensor using streptavidin-conjugated quantum dots (QDs/SA) was developed to detect dengue biomarker of non-structural protein 1 (NS1) at very low concentration, so that it can probe dengue infection even in the early stage. The QDs/SA were first bound to biotinylated NS1 antibody (Ab) and the QDs/SA-Ab conjugates were then used to detect the NS1 antigen (Ag) in the Ag concentration range of 1 pM to 120 nM. The formation of QDs/SA-Ab and QDs/SA-Ab-Ag conjugates was confirmed by the measurements of field emission scanning electron microscopy (FF-SEM), field emission transmission electron microscopy (FE-TEM), dynamic light scattering (DLS), and zeta-potential. Fluorescence emission spectra of QDs/SA-Ab-Ag conjugates showed that the magnitude of fluorescence quenching was linearly proportional to the NS1 Ag concentration and it nicely followed the Stern–Volmer (SV) equation in phosphate buffer solution. However, in human plasma serum solution, the fluorescence quenching behavior was negatively deviated from the SV equation presumably due to interference by the serum component biomolecules, and it was well explained by the Lehrer equation. These results suggest that the current approach is promising because it is highly sensitive, fast, simple, and convenient, and thus it has a potential of application for point-of-care.Linh TranSangkwon ParkNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Linh Tran Sangkwon Park Highly sensitive detection of dengue biomarker using streptavidin-conjugated quantum dots
description	Abstract A highly sensitive immunosensor using streptavidin-conjugated quantum dots (QDs/SA) was developed to detect dengue biomarker of non-structural protein 1 (NS1) at very low concentration, so that it can probe dengue infection even in the early stage. The QDs/SA were first bound to biotinylated NS1 antibody (Ab) and the QDs/SA-Ab conjugates were then used to detect the NS1 antigen (Ag) in the Ag concentration range of 1 pM to 120 nM. The formation of QDs/SA-Ab and QDs/SA-Ab-Ag conjugates was confirmed by the measurements of field emission scanning electron microscopy (FF-SEM), field emission transmission electron microscopy (FE-TEM), dynamic light scattering (DLS), and zeta-potential. Fluorescence emission spectra of QDs/SA-Ab-Ag conjugates showed that the magnitude of fluorescence quenching was linearly proportional to the NS1 Ag concentration and it nicely followed the Stern–Volmer (SV) equation in phosphate buffer solution. However, in human plasma serum solution, the fluorescence quenching behavior was negatively deviated from the SV equation presumably due to interference by the serum component biomolecules, and it was well explained by the Lehrer equation. These results suggest that the current approach is promising because it is highly sensitive, fast, simple, and convenient, and thus it has a potential of application for point-of-care.
format	article
author	Linh Tran Sangkwon Park
author_facet	Linh Tran Sangkwon Park
author_sort	Linh Tran
title	Highly sensitive detection of dengue biomarker using streptavidin-conjugated quantum dots
title_short	Highly sensitive detection of dengue biomarker using streptavidin-conjugated quantum dots
title_full	Highly sensitive detection of dengue biomarker using streptavidin-conjugated quantum dots
title_fullStr	Highly sensitive detection of dengue biomarker using streptavidin-conjugated quantum dots
title_full_unstemmed	Highly sensitive detection of dengue biomarker using streptavidin-conjugated quantum dots
title_sort	highly sensitive detection of dengue biomarker using streptavidin-conjugated quantum dots
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/6cf34517d3d84b638e0193b3ca5c3842
work_keys_str_mv	AT linhtran highlysensitivedetectionofdenguebiomarkerusingstreptavidinconjugatedquantumdots AT sangkwonpark highlysensitivedetectionofdenguebiomarkerusingstreptavidinconjugatedquantumdots
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Highly sensitive detection of dengue biomarker using streptavidin-conjugated quantum dots

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