Ultra-high-resolution computed tomography shows changes in the lungs related with airway hyperresponsiveness in a murine asthma model

Abstract In vivo presentation of airway hyper-responsiveness (AHR) at the different time points of the allergic reaction is not clearly understood. The purpose of this study was to investigate how AHR manifests in the airway and the lung parenchyma in vivo following exposure to different stimuli and...

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Autores principales:	Jae-Woo Jung, Jung Suk Oh, Boram Bae, Yoon Hae Ahn, Lucy Wooyeon Kim, Jiwoong Choi, Hye-Young Kim, Hye-Ryun Kang, Chang Hyun Lee
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6cf79257e9794ab0afca3528e8ed5a82
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spelling	oai:doaj.org-article:6cf79257e9794ab0afca3528e8ed5a822021-12-02T15:28:46ZUltra-high-resolution computed tomography shows changes in the lungs related with airway hyperresponsiveness in a murine asthma model10.1038/s41598-021-96853-z2045-2322https://doaj.org/article/6cf79257e9794ab0afca3528e8ed5a822021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-96853-zhttps://doaj.org/toc/2045-2322Abstract In vivo presentation of airway hyper-responsiveness (AHR) at the different time points of the allergic reaction is not clearly understood. The purpose of this study was to investigate how AHR manifests in the airway and the lung parenchyma in vivo following exposure to different stimuli and in the early and late phases of asthma after allergen exposure. Ovalbumin (OVA)-induced allergic asthma model was established using 6-week female BALB/c mice. Enhanced pause was measured with a non-invasive method to assess AHR. The dynamic changes of the airway and lung parenchyma were evaluated with ultra-high-resolution computed tomography (128 multi-detector, 1024 × 1024 matrix) for 10 h. While the methacholine challenge showed no grossly visible changes in the proximal airway and lung parenchyma despite provoking AHR, the OVA challenge induced significant immediate changes manifesting as peribronchial ground glass opacities, consolidations, air-trapping, and paradoxical proximal airway dilatations. After resolution of immediate response, multiple episodes of AHRs occurred with paradoxical proximal airway dilatation and peripheral air-trapping in late phase over a prolonged time period in vivo. Understanding of airflow limitation based on the structural changes of asthmatic airway would be helpful to make an appropriate drug delivery strategy for the treatment of asthma.Jae-Woo JungJung Suk OhBoram BaeYoon Hae AhnLucy Wooyeon KimJiwoong ChoiHye-Young KimHye-Ryun KangChang Hyun LeeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jae-Woo Jung Jung Suk Oh Boram Bae Yoon Hae Ahn Lucy Wooyeon Kim Jiwoong Choi Hye-Young Kim Hye-Ryun Kang Chang Hyun Lee Ultra-high-resolution computed tomography shows changes in the lungs related with airway hyperresponsiveness in a murine asthma model
description	Abstract In vivo presentation of airway hyper-responsiveness (AHR) at the different time points of the allergic reaction is not clearly understood. The purpose of this study was to investigate how AHR manifests in the airway and the lung parenchyma in vivo following exposure to different stimuli and in the early and late phases of asthma after allergen exposure. Ovalbumin (OVA)-induced allergic asthma model was established using 6-week female BALB/c mice. Enhanced pause was measured with a non-invasive method to assess AHR. The dynamic changes of the airway and lung parenchyma were evaluated with ultra-high-resolution computed tomography (128 multi-detector, 1024 × 1024 matrix) for 10 h. While the methacholine challenge showed no grossly visible changes in the proximal airway and lung parenchyma despite provoking AHR, the OVA challenge induced significant immediate changes manifesting as peribronchial ground glass opacities, consolidations, air-trapping, and paradoxical proximal airway dilatations. After resolution of immediate response, multiple episodes of AHRs occurred with paradoxical proximal airway dilatation and peripheral air-trapping in late phase over a prolonged time period in vivo. Understanding of airflow limitation based on the structural changes of asthmatic airway would be helpful to make an appropriate drug delivery strategy for the treatment of asthma.
format	article
author	Jae-Woo Jung Jung Suk Oh Boram Bae Yoon Hae Ahn Lucy Wooyeon Kim Jiwoong Choi Hye-Young Kim Hye-Ryun Kang Chang Hyun Lee
author_facet	Jae-Woo Jung Jung Suk Oh Boram Bae Yoon Hae Ahn Lucy Wooyeon Kim Jiwoong Choi Hye-Young Kim Hye-Ryun Kang Chang Hyun Lee
author_sort	Jae-Woo Jung
title	Ultra-high-resolution computed tomography shows changes in the lungs related with airway hyperresponsiveness in a murine asthma model
title_short	Ultra-high-resolution computed tomography shows changes in the lungs related with airway hyperresponsiveness in a murine asthma model
title_full	Ultra-high-resolution computed tomography shows changes in the lungs related with airway hyperresponsiveness in a murine asthma model
title_fullStr	Ultra-high-resolution computed tomography shows changes in the lungs related with airway hyperresponsiveness in a murine asthma model
title_full_unstemmed	Ultra-high-resolution computed tomography shows changes in the lungs related with airway hyperresponsiveness in a murine asthma model
title_sort	ultra-high-resolution computed tomography shows changes in the lungs related with airway hyperresponsiveness in a murine asthma model
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/6cf79257e9794ab0afca3528e8ed5a82
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Ultra-high-resolution computed tomography shows changes in the lungs related with airway hyperresponsiveness in a murine asthma model

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