Molecular phylogenetic inference of White-Spotted Guitarfish (Rhynchobatus australiae) collected from local Malaysian fish markets

Md-Zain BM, Abdul-Mutalib SA, Aifat NR, Masstor NH, Mohd-Yusof NS, Mohd-Hashim A, Abdul-Latiff MAB, Yaakop S, Samat A. 2018. Molecular phylogenetic inference of White-Spotted Guitarfish (Rhynchobatus australiae) collected from local Malaysian fish markets. Biodiversitas 19: 1382-1386. The white-spot...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	BADRUL MUNIR MD-ZAIN, SHAHIDATINA AISHAH ABDUL-MUTALIB
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2018
Materias:	phylogenetic position ray rhyncobatus australiae shark white-spotted guitarfish Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/6d090d3055c14e028d7f043281f0d9c7
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:6d090d3055c14e028d7f043281f0d9c7
record_format	dspace
spelling	oai:doaj.org-article:6d090d3055c14e028d7f043281f0d9c72021-11-16T14:03:29ZMolecular phylogenetic inference of White-Spotted Guitarfish (Rhynchobatus australiae) collected from local Malaysian fish markets1412-033X2085-472210.13057/biodiv/d190426https://doaj.org/article/6d090d3055c14e028d7f043281f0d9c72018-07-01T00:00:00Zhttps://smujo.id/biodiv/article/view/2797https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Md-Zain BM, Abdul-Mutalib SA, Aifat NR, Masstor NH, Mohd-Yusof NS, Mohd-Hashim A, Abdul-Latiff MAB, Yaakop S, Samat A. 2018. Molecular phylogenetic inference of White-Spotted Guitarfish (Rhynchobatus australiae) collected from local Malaysian fish markets. Biodiversitas 19: 1382-1386. The white-spotted guitarfish (Rhynchobatus australiae) is in high demand at local Malaysian fish markets because its fins are a valuable food source. To date, few molecular studies have characterized their genetic identity. We have conducted a molecular study to infer the phylogenetic relationships of white-spotted guitarfish, which portray a similar morphology to sharks and rays. The main objective of this study was to determine the phylogenetic position of R. australiae using cytochrome oxidase I (COI) sequences of mitochondrial DNA based on fish samples collected from local Malaysian fish markets. This study included nine genetic samples of R. australiae and fourteen samples from other members of the shark and ray families, including Sphyrna lewini (Sphyrnidae), Rhizoprionodon oligolinx and Carcharhinus sorrah (Carcharhinidae), Dasyatis zugei, Himantura walga, Himantura gerradi, Himantura jenkinsii and Neotrygon kuhlii (Dasyatidae). Chimaera fulva, a member of the Chimaera family, was used as the outgroup. Sequences in size of ~701 base pairs were successfully obtained from all fish samples. The phylogenetic tree topology was reconstructed using distance-based (neighbor-joining) and character-based (maximum parsimony) methods using MEGA and PAUP software. Results indicated that R. australiae formed monophyletic clade and is closely related to sharks (Sphyrnidae and Carcharhinidae). This conclusion was also supported by genetic distance analysis which indicated that Rhynchobatidae and sharks (Carcharhinidae and Sphyrnidae) were closer to each other than to rays (Dasyatidae). This study has proven the efficiency of the COI mitochondrial locus in revealing the phylogenetic position of R. australiae. Research findings from this study have increased our understanding of the phylogenetic relationships among guitarfish, sharks, and rays, and their respective taxonomic positions are given their shared morphological characters. This will benefit us in identifying these fish species before consumption from local fish markets.BADRUL MUNIR MD-ZAINSHAHIDATINA AISHAH ABDUL-MUTALIBMBI & UNS Soloarticlephylogenetic positionrayrhyncobatus australiaesharkwhite-spotted guitarfishBiology (General)QH301-705.5ENBiodiversitas, Vol 19, Iss 4, Pp 1382-1386 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	phylogenetic position ray rhyncobatus australiae shark white-spotted guitarfish Biology (General) QH301-705.5
spellingShingle	phylogenetic position ray rhyncobatus australiae shark white-spotted guitarfish Biology (General) QH301-705.5 BADRUL MUNIR MD-ZAIN SHAHIDATINA AISHAH ABDUL-MUTALIB Molecular phylogenetic inference of White-Spotted Guitarfish (Rhynchobatus australiae) collected from local Malaysian fish markets
description	Md-Zain BM, Abdul-Mutalib SA, Aifat NR, Masstor NH, Mohd-Yusof NS, Mohd-Hashim A, Abdul-Latiff MAB, Yaakop S, Samat A. 2018. Molecular phylogenetic inference of White-Spotted Guitarfish (Rhynchobatus australiae) collected from local Malaysian fish markets. Biodiversitas 19: 1382-1386. The white-spotted guitarfish (Rhynchobatus australiae) is in high demand at local Malaysian fish markets because its fins are a valuable food source. To date, few molecular studies have characterized their genetic identity. We have conducted a molecular study to infer the phylogenetic relationships of white-spotted guitarfish, which portray a similar morphology to sharks and rays. The main objective of this study was to determine the phylogenetic position of R. australiae using cytochrome oxidase I (COI) sequences of mitochondrial DNA based on fish samples collected from local Malaysian fish markets. This study included nine genetic samples of R. australiae and fourteen samples from other members of the shark and ray families, including Sphyrna lewini (Sphyrnidae), Rhizoprionodon oligolinx and Carcharhinus sorrah (Carcharhinidae), Dasyatis zugei, Himantura walga, Himantura gerradi, Himantura jenkinsii and Neotrygon kuhlii (Dasyatidae). Chimaera fulva, a member of the Chimaera family, was used as the outgroup. Sequences in size of ~701 base pairs were successfully obtained from all fish samples. The phylogenetic tree topology was reconstructed using distance-based (neighbor-joining) and character-based (maximum parsimony) methods using MEGA and PAUP software. Results indicated that R. australiae formed monophyletic clade and is closely related to sharks (Sphyrnidae and Carcharhinidae). This conclusion was also supported by genetic distance analysis which indicated that Rhynchobatidae and sharks (Carcharhinidae and Sphyrnidae) were closer to each other than to rays (Dasyatidae). This study has proven the efficiency of the COI mitochondrial locus in revealing the phylogenetic position of R. australiae. Research findings from this study have increased our understanding of the phylogenetic relationships among guitarfish, sharks, and rays, and their respective taxonomic positions are given their shared morphological characters. This will benefit us in identifying these fish species before consumption from local fish markets.
format	article
author	BADRUL MUNIR MD-ZAIN SHAHIDATINA AISHAH ABDUL-MUTALIB
author_facet	BADRUL MUNIR MD-ZAIN SHAHIDATINA AISHAH ABDUL-MUTALIB
author_sort	BADRUL MUNIR MD-ZAIN
title	Molecular phylogenetic inference of White-Spotted Guitarfish (Rhynchobatus australiae) collected from local Malaysian fish markets
title_short	Molecular phylogenetic inference of White-Spotted Guitarfish (Rhynchobatus australiae) collected from local Malaysian fish markets
title_full	Molecular phylogenetic inference of White-Spotted Guitarfish (Rhynchobatus australiae) collected from local Malaysian fish markets
title_fullStr	Molecular phylogenetic inference of White-Spotted Guitarfish (Rhynchobatus australiae) collected from local Malaysian fish markets
title_full_unstemmed	Molecular phylogenetic inference of White-Spotted Guitarfish (Rhynchobatus australiae) collected from local Malaysian fish markets
title_sort	molecular phylogenetic inference of white-spotted guitarfish (rhynchobatus australiae) collected from local malaysian fish markets
publisher	MBI & UNS Solo
publishDate	2018
url	https://doaj.org/article/6d090d3055c14e028d7f043281f0d9c7
work_keys_str_mv	AT badrulmunirmdzain molecularphylogeneticinferenceofwhitespottedguitarfishrhynchobatusaustraliaecollectedfromlocalmalaysianfishmarkets AT shahidatinaaishahabdulmutalib molecularphylogeneticinferenceofwhitespottedguitarfishrhynchobatusaustraliaecollectedfromlocalmalaysianfishmarkets
_version_	1718426428681224192

Molecular phylogenetic inference of White-Spotted Guitarfish (Rhynchobatus australiae) collected from local Malaysian fish markets

Ejemplares similares