A five-species transcriptome array for oral mixed-biofilm studies.

<h4>Background</h4>Oral polymicrobial interactions and biofilm formation are associated with initiation and progression of caries, gingivitis, and periodontitis. Transcriptome studies of such interactions, allowing a first mechanistic insight, are hampered by current single-species array...

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Autores principales:	Sylvio Redanz, Kerstin Standar, Andreas Podbielski, Bernd Kreikemeyer
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6d0c13e5de2c41b2b2358b020f1d6356
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spelling	oai:doaj.org-article:6d0c13e5de2c41b2b2358b020f1d63562021-11-18T07:32:23ZA five-species transcriptome array for oral mixed-biofilm studies.1932-620310.1371/journal.pone.0027827https://doaj.org/article/6d0c13e5de2c41b2b2358b020f1d63562011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22194794/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Oral polymicrobial interactions and biofilm formation are associated with initiation and progression of caries, gingivitis, and periodontitis. Transcriptome studies of such interactions, allowing a first mechanistic insight, are hampered by current single-species array designs.<h4>Methodology/principal findings</h4>In this study we used 385 K NimbleGene™ technology for design and evaluation of an array covering the full genomes of 5 important physiological-, cariogenic-, and periodontitis-associated microorganisms (Streptococcus sanguinis, Streptococcus mutans, Fusobacterium nucleatum, Aggregatibacter actinomycetemcomitans, and Porphyromonas gingivalis). Array hybridization was done with cDNA from cultures grown for 24 h anaerobically. Single species experiments identified cross-species hybridizing array probes. These probes could be neglected in a mixed-species experimental setting without the need to exclude the whole genes from the analysis. Between 69% and almost 99% of the genomes were actively transcribed under the mono-species planktonic, monolayer, and biofilm conditions. The influence of Streptococcus mitis (not represented on the array) on S. mutans gene transcription was determined as a test for a dual-species mixed biofilm setup. Phenotypically, under the influence of S. mitis an increase in S. mutans biofilm mass and a decrease in media pH-value were noticed, thereby confirming previously published data. Employing a stringent cut-off (2-fold, p<0.05), 19 S. mutans transcripts were identified with increased abundance, and 11 with decreased abundance compared to a S. mutans mono-species biofilm. Several of these genes have previously been found differentially regulated under general and acid stress, thereby confirming the value of this array.<h4>Conclusions/significance</h4>This new array allows transcriptome studies on multi-species oral biofilm interactions. It may become an important asset in future oral biofilm and inhibitor/therapy studies.Sylvio RedanzKerstin StandarAndreas PodbielskiBernd KreikemeyerPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 12, p e27827 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sylvio Redanz Kerstin Standar Andreas Podbielski Bernd Kreikemeyer A five-species transcriptome array for oral mixed-biofilm studies.
description	<h4>Background</h4>Oral polymicrobial interactions and biofilm formation are associated with initiation and progression of caries, gingivitis, and periodontitis. Transcriptome studies of such interactions, allowing a first mechanistic insight, are hampered by current single-species array designs.<h4>Methodology/principal findings</h4>In this study we used 385 K NimbleGene™ technology for design and evaluation of an array covering the full genomes of 5 important physiological-, cariogenic-, and periodontitis-associated microorganisms (Streptococcus sanguinis, Streptococcus mutans, Fusobacterium nucleatum, Aggregatibacter actinomycetemcomitans, and Porphyromonas gingivalis). Array hybridization was done with cDNA from cultures grown for 24 h anaerobically. Single species experiments identified cross-species hybridizing array probes. These probes could be neglected in a mixed-species experimental setting without the need to exclude the whole genes from the analysis. Between 69% and almost 99% of the genomes were actively transcribed under the mono-species planktonic, monolayer, and biofilm conditions. The influence of Streptococcus mitis (not represented on the array) on S. mutans gene transcription was determined as a test for a dual-species mixed biofilm setup. Phenotypically, under the influence of S. mitis an increase in S. mutans biofilm mass and a decrease in media pH-value were noticed, thereby confirming previously published data. Employing a stringent cut-off (2-fold, p<0.05), 19 S. mutans transcripts were identified with increased abundance, and 11 with decreased abundance compared to a S. mutans mono-species biofilm. Several of these genes have previously been found differentially regulated under general and acid stress, thereby confirming the value of this array.<h4>Conclusions/significance</h4>This new array allows transcriptome studies on multi-species oral biofilm interactions. It may become an important asset in future oral biofilm and inhibitor/therapy studies.
format	article
author	Sylvio Redanz Kerstin Standar Andreas Podbielski Bernd Kreikemeyer
author_facet	Sylvio Redanz Kerstin Standar Andreas Podbielski Bernd Kreikemeyer
author_sort	Sylvio Redanz
title	A five-species transcriptome array for oral mixed-biofilm studies.
title_short	A five-species transcriptome array for oral mixed-biofilm studies.
title_full	A five-species transcriptome array for oral mixed-biofilm studies.
title_fullStr	A five-species transcriptome array for oral mixed-biofilm studies.
title_full_unstemmed	A five-species transcriptome array for oral mixed-biofilm studies.
title_sort	five-species transcriptome array for oral mixed-biofilm studies.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/6d0c13e5de2c41b2b2358b020f1d6356
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A five-species transcriptome array for oral mixed-biofilm studies.

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