Hymyc1 downregulation promotes stem cell proliferation in Hydra vulgaris.

Hydra is a unique model for studying the mechanisms underlying stem cell biology. The activity of the three stem cell lineages structuring its body constantly replenishes mature cells lost due to normal tissue turnover. By a poorly understood mechanism, stem cells are maintained through self-renewal...

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Autores principales:	Alfredo Ambrosone, Valentina Marchesano, Angela Tino, Bert Hobmayer, Claudia Tortiglione
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6d0fc4b42dc1461cba80049d356ca6dd
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spelling	oai:doaj.org-article:6d0fc4b42dc1461cba80049d356ca6dd2021-11-18T07:29:34ZHymyc1 downregulation promotes stem cell proliferation in Hydra vulgaris.1932-620310.1371/journal.pone.0030660https://doaj.org/article/6d0fc4b42dc1461cba80049d356ca6dd2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22292012/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Hydra is a unique model for studying the mechanisms underlying stem cell biology. The activity of the three stem cell lineages structuring its body constantly replenishes mature cells lost due to normal tissue turnover. By a poorly understood mechanism, stem cells are maintained through self-renewal while concomitantly producing differentiated progeny. In vertebrates, one of many genes that participate in regulating stem cell homeostasis is the protooncogene c-myc, which has been recently identified also in Hydra, and found expressed in the interstitial stem cell lineage. In the present paper, by developing a novel strategy of RNA interference-mediated gene silencing (RNAi) based on an enhanced uptake of small interfering RNAi (siRNA), we provide molecular and biological evidence for an unexpected function of the Hydra myc gene (Hymyc1) in the homeostasis of the interstitial stem cell lineage. We found that Hymyc1 inhibition impairs the balance between stem cell self renewal/differentiation, as shown by the accumulation of stem cell intermediate and terminal differentiation products in genetically interfered animals. The identical phenotype induced by the 10058-F4 inhibitor, a disruptor of c-Myc/Max dimerization, demonstrates the specificity of the RNAi approach. We show the kinetic and the reversible feature of Hymyc1 RNAi, together with the effects displayed on regenerating animals. Our results show the involvement of Hymyc1 in the control of interstitial stem cell dynamics, provide new clues to decipher the molecular control of the cell and tissue plasticity in Hydra, and also provide further insights into the complex myc network in higher organisms. The ability of Hydra cells to uptake double stranded RNA and to trigger a RNAi response lays the foundations of a comprehensive analysis of the RNAi response in Hydra allowing us to track back in the evolution and the origin of this process.Alfredo AmbrosoneValentina MarchesanoAngela TinoBert HobmayerClaudia TortiglionePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 1, p e30660 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Alfredo Ambrosone Valentina Marchesano Angela Tino Bert Hobmayer Claudia Tortiglione Hymyc1 downregulation promotes stem cell proliferation in Hydra vulgaris.
description	Hydra is a unique model for studying the mechanisms underlying stem cell biology. The activity of the three stem cell lineages structuring its body constantly replenishes mature cells lost due to normal tissue turnover. By a poorly understood mechanism, stem cells are maintained through self-renewal while concomitantly producing differentiated progeny. In vertebrates, one of many genes that participate in regulating stem cell homeostasis is the protooncogene c-myc, which has been recently identified also in Hydra, and found expressed in the interstitial stem cell lineage. In the present paper, by developing a novel strategy of RNA interference-mediated gene silencing (RNAi) based on an enhanced uptake of small interfering RNAi (siRNA), we provide molecular and biological evidence for an unexpected function of the Hydra myc gene (Hymyc1) in the homeostasis of the interstitial stem cell lineage. We found that Hymyc1 inhibition impairs the balance between stem cell self renewal/differentiation, as shown by the accumulation of stem cell intermediate and terminal differentiation products in genetically interfered animals. The identical phenotype induced by the 10058-F4 inhibitor, a disruptor of c-Myc/Max dimerization, demonstrates the specificity of the RNAi approach. We show the kinetic and the reversible feature of Hymyc1 RNAi, together with the effects displayed on regenerating animals. Our results show the involvement of Hymyc1 in the control of interstitial stem cell dynamics, provide new clues to decipher the molecular control of the cell and tissue plasticity in Hydra, and also provide further insights into the complex myc network in higher organisms. The ability of Hydra cells to uptake double stranded RNA and to trigger a RNAi response lays the foundations of a comprehensive analysis of the RNAi response in Hydra allowing us to track back in the evolution and the origin of this process.
format	article
author	Alfredo Ambrosone Valentina Marchesano Angela Tino Bert Hobmayer Claudia Tortiglione
author_facet	Alfredo Ambrosone Valentina Marchesano Angela Tino Bert Hobmayer Claudia Tortiglione
author_sort	Alfredo Ambrosone
title	Hymyc1 downregulation promotes stem cell proliferation in Hydra vulgaris.
title_short	Hymyc1 downregulation promotes stem cell proliferation in Hydra vulgaris.
title_full	Hymyc1 downregulation promotes stem cell proliferation in Hydra vulgaris.
title_fullStr	Hymyc1 downregulation promotes stem cell proliferation in Hydra vulgaris.
title_full_unstemmed	Hymyc1 downregulation promotes stem cell proliferation in Hydra vulgaris.
title_sort	hymyc1 downregulation promotes stem cell proliferation in hydra vulgaris.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/6d0fc4b42dc1461cba80049d356ca6dd
work_keys_str_mv	AT alfredoambrosone hymyc1downregulationpromotesstemcellproliferationinhydravulgaris AT valentinamarchesano hymyc1downregulationpromotesstemcellproliferationinhydravulgaris AT angelatino hymyc1downregulationpromotesstemcellproliferationinhydravulgaris AT berthobmayer hymyc1downregulationpromotesstemcellproliferationinhydravulgaris AT claudiatortiglione hymyc1downregulationpromotesstemcellproliferationinhydravulgaris
_version_	1718423349461254144

Hymyc1 downregulation promotes stem cell proliferation in Hydra vulgaris.

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