The natural Disc1-deletion present in several inbred mouse strains does not affect sleep

Abstract The gene Disrupted in Schizophrenia-1 (DISC1) is linked to a range of psychiatric disorders. Two recent transgenic studies suggest DISC1 is also involved in homeostatic sleep regulation. Several strains of inbred mice commonly used for genome manipulation experiments, including several Swis...

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Autores principales:	Lars Dittrich, Alessandro Petese, Walker S. Jackson
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6d1637fccaeb478da9869ef66470825c
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spelling	oai:doaj.org-article:6d1637fccaeb478da9869ef66470825c2021-12-02T15:06:06ZThe natural Disc1-deletion present in several inbred mouse strains does not affect sleep10.1038/s41598-017-06015-32045-2322https://doaj.org/article/6d1637fccaeb478da9869ef66470825c2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06015-3https://doaj.org/toc/2045-2322Abstract The gene Disrupted in Schizophrenia-1 (DISC1) is linked to a range of psychiatric disorders. Two recent transgenic studies suggest DISC1 is also involved in homeostatic sleep regulation. Several strains of inbred mice commonly used for genome manipulation experiments, including several Swiss and likely all 129 substrains, carry a natural deletion mutation of Disc1. This constitutes a potential confound for studying sleep in genetically modified mice. Since disturbed sleep can also influence psychiatric and neurodegenerative disease models, this putative confound might affect a wide range of studies in several fields. Therefore, we asked to what extent the natural Disc1 deletion affects sleep. To this end, we first compared sleep and electroencephalogram (EEG) phenotypes of 129S4 mice carrying the Disc1 deletion and C57BL/6N mice carrying the full-length version. We then bred Disc1 from C57BL/6N into the 129S4 background, resulting in S4-Disc1 mice. The differences between 129S4 and C57BL/6N were not detected in the 129S4 to S4-Disc1 comparison. We conclude that the mutation has no effect on the measured sleep and EEG characteristics. Thus, it is unlikely the widespread Disc1 deletion has led to spurious results in previous sleep studies or that it alters sleep in mouse models of psychiatric or neurodegenerative diseases.Lars DittrichAlessandro PeteseWalker S. JacksonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lars Dittrich Alessandro Petese Walker S. Jackson The natural Disc1-deletion present in several inbred mouse strains does not affect sleep
description	Abstract The gene Disrupted in Schizophrenia-1 (DISC1) is linked to a range of psychiatric disorders. Two recent transgenic studies suggest DISC1 is also involved in homeostatic sleep regulation. Several strains of inbred mice commonly used for genome manipulation experiments, including several Swiss and likely all 129 substrains, carry a natural deletion mutation of Disc1. This constitutes a potential confound for studying sleep in genetically modified mice. Since disturbed sleep can also influence psychiatric and neurodegenerative disease models, this putative confound might affect a wide range of studies in several fields. Therefore, we asked to what extent the natural Disc1 deletion affects sleep. To this end, we first compared sleep and electroencephalogram (EEG) phenotypes of 129S4 mice carrying the Disc1 deletion and C57BL/6N mice carrying the full-length version. We then bred Disc1 from C57BL/6N into the 129S4 background, resulting in S4-Disc1 mice. The differences between 129S4 and C57BL/6N were not detected in the 129S4 to S4-Disc1 comparison. We conclude that the mutation has no effect on the measured sleep and EEG characteristics. Thus, it is unlikely the widespread Disc1 deletion has led to spurious results in previous sleep studies or that it alters sleep in mouse models of psychiatric or neurodegenerative diseases.
format	article
author	Lars Dittrich Alessandro Petese Walker S. Jackson
author_facet	Lars Dittrich Alessandro Petese Walker S. Jackson
author_sort	Lars Dittrich
title	The natural Disc1-deletion present in several inbred mouse strains does not affect sleep
title_short	The natural Disc1-deletion present in several inbred mouse strains does not affect sleep
title_full	The natural Disc1-deletion present in several inbred mouse strains does not affect sleep
title_fullStr	The natural Disc1-deletion present in several inbred mouse strains does not affect sleep
title_full_unstemmed	The natural Disc1-deletion present in several inbred mouse strains does not affect sleep
title_sort	natural disc1-deletion present in several inbred mouse strains does not affect sleep
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/6d1637fccaeb478da9869ef66470825c
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The natural Disc1-deletion present in several inbred mouse strains does not affect sleep

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