The effect of macrophage polarization on the expression of the oxytocin signalling system in enteric neurons

Abstract Background The aim of the current study was to investigate the effect of macrophage polarization on the expression of oxytocin (OT) and the oxytocin receptor (OTR) in enteric neurons. Methods In this study, we used a classic colitis model and D-mannose model to observe the correlation betwe...

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Autores principales:	Yao Shi, Shuang Li, Haojie Zhang, Jianchun Zhu, Tongtong Che, Bing Yan, Jingxin Li, Chuanyong Liu
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	OT signalling system Macrophage polarization Proinflammatory and anti-inflammatory factors Neural-immune interactions Neurology. Diseases of the nervous system RC346-429
Acceso en línea:	https://doaj.org/article/6d1b425c9d934e96b787751ccf322c5a
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spelling	oai:doaj.org-article:6d1b425c9d934e96b787751ccf322c5a2021-11-14T12:38:07ZThe effect of macrophage polarization on the expression of the oxytocin signalling system in enteric neurons10.1186/s12974-021-02313-w1742-2094https://doaj.org/article/6d1b425c9d934e96b787751ccf322c5a2021-11-01T00:00:00Zhttps://doi.org/10.1186/s12974-021-02313-whttps://doaj.org/toc/1742-2094Abstract Background The aim of the current study was to investigate the effect of macrophage polarization on the expression of oxytocin (OT) and the oxytocin receptor (OTR) in enteric neurons. Methods In this study, we used a classic colitis model and D-mannose model to observe the correlation between macrophage polarization and OT signalling system. In order to further demonstrate the effect of macrophages, we examined the expression of OT signalling system after depletion of macrophages. Results The data showed that, in vitro, following polarization of macrophages to the M1 type by LPS, the macrophage supernatant contained proinflammatory cytokines (IL-1β, IL-6 and TNF-α) that inhibited the expression of OT and OTR in cultured enteric neurons; following macrophage polarization to the M2 type by IL4, the macrophage supernatant contained anti-inflammatory cytokines (TGF-β) that promoted the expression of OT and OTR in cultured enteric neurons. Furthermore, M1 macrophages decreased the expression of the OT signalling system mainly through STAT3/NF-κB pathways in cultured enteric neurons; M2 macrophages increased the expression of the OT signalling system mainly through activation of Smad2/3 and inhibition of the expression of Peg3 in cultured enteric neurons. In a colitis model, we demonstrated that macrophages were polarized to the M1 type during the inflammatory phase, with significant decreased in the expression of OT and OTR. When macrophages were polarized to the M2 type during the recovery phase, OT and OTR expression increased significantly. In addition, we found that D-mannose increased the expression of OT and OTR through polarization of macrophages to the M2 type. Conclusions This is the first study to demonstrate that macrophage polarization differentially regulates the expression of OT and OTR in enteric neurons.Yao ShiShuang LiHaojie ZhangJianchun ZhuTongtong CheBing YanJingxin LiChuanyong LiuBMCarticleOT signalling systemMacrophage polarizationProinflammatory and anti-inflammatory factorsNeural-immune interactionsNeurology. Diseases of the nervous systemRC346-429ENJournal of Neuroinflammation, Vol 18, Iss 1, Pp 1-18 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	OT signalling system Macrophage polarization Proinflammatory and anti-inflammatory factors Neural-immune interactions Neurology. Diseases of the nervous system RC346-429
spellingShingle	OT signalling system Macrophage polarization Proinflammatory and anti-inflammatory factors Neural-immune interactions Neurology. Diseases of the nervous system RC346-429 Yao Shi Shuang Li Haojie Zhang Jianchun Zhu Tongtong Che Bing Yan Jingxin Li Chuanyong Liu The effect of macrophage polarization on the expression of the oxytocin signalling system in enteric neurons
description	Abstract Background The aim of the current study was to investigate the effect of macrophage polarization on the expression of oxytocin (OT) and the oxytocin receptor (OTR) in enteric neurons. Methods In this study, we used a classic colitis model and D-mannose model to observe the correlation between macrophage polarization and OT signalling system. In order to further demonstrate the effect of macrophages, we examined the expression of OT signalling system after depletion of macrophages. Results The data showed that, in vitro, following polarization of macrophages to the M1 type by LPS, the macrophage supernatant contained proinflammatory cytokines (IL-1β, IL-6 and TNF-α) that inhibited the expression of OT and OTR in cultured enteric neurons; following macrophage polarization to the M2 type by IL4, the macrophage supernatant contained anti-inflammatory cytokines (TGF-β) that promoted the expression of OT and OTR in cultured enteric neurons. Furthermore, M1 macrophages decreased the expression of the OT signalling system mainly through STAT3/NF-κB pathways in cultured enteric neurons; M2 macrophages increased the expression of the OT signalling system mainly through activation of Smad2/3 and inhibition of the expression of Peg3 in cultured enteric neurons. In a colitis model, we demonstrated that macrophages were polarized to the M1 type during the inflammatory phase, with significant decreased in the expression of OT and OTR. When macrophages were polarized to the M2 type during the recovery phase, OT and OTR expression increased significantly. In addition, we found that D-mannose increased the expression of OT and OTR through polarization of macrophages to the M2 type. Conclusions This is the first study to demonstrate that macrophage polarization differentially regulates the expression of OT and OTR in enteric neurons.
format	article
author	Yao Shi Shuang Li Haojie Zhang Jianchun Zhu Tongtong Che Bing Yan Jingxin Li Chuanyong Liu
author_facet	Yao Shi Shuang Li Haojie Zhang Jianchun Zhu Tongtong Che Bing Yan Jingxin Li Chuanyong Liu
author_sort	Yao Shi
title	The effect of macrophage polarization on the expression of the oxytocin signalling system in enteric neurons
title_short	The effect of macrophage polarization on the expression of the oxytocin signalling system in enteric neurons
title_full	The effect of macrophage polarization on the expression of the oxytocin signalling system in enteric neurons
title_fullStr	The effect of macrophage polarization on the expression of the oxytocin signalling system in enteric neurons
title_full_unstemmed	The effect of macrophage polarization on the expression of the oxytocin signalling system in enteric neurons
title_sort	effect of macrophage polarization on the expression of the oxytocin signalling system in enteric neurons
publisher	BMC
publishDate	2021
url	https://doaj.org/article/6d1b425c9d934e96b787751ccf322c5a
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The effect of macrophage polarization on the expression of the oxytocin signalling system in enteric neurons

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