Correlations between the Composition of the Bovine Microbiota and Vitamin B<sub>12</sub> Abundance

ABSTRACT Vitamin B12 is synthesized by prokaryotes in the rumens of dairy cows—and this has implications in human nutrition since humans rely on consumption of dairy products for vitamin B12 acquisition. However, the concentration of vitamin B12 in milk is highly variable, and there is interest in d...

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Autores principales:	Julian Franco-Lopez, Melissa Duplessis, An Bui, Coralie Reymond, William Poisson, Lya Blais, Jasmine Chong, Rachel Gervais, Daniel E. Rico, Roger I. Cue, Christiane L. Girard, Jennifer Ronholm
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2020
Materias:	bovine microbiome microbiota milk rumen vitamin B12 Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/6d210c2ae2364d619c380ad3dfce73ce
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spelling	oai:doaj.org-article:6d210c2ae2364d619c380ad3dfce73ce2021-12-02T18:15:46ZCorrelations between the Composition of the Bovine Microbiota and Vitamin B<sub>12</sub> Abundance10.1128/mSystems.00107-202379-5077https://doaj.org/article/6d210c2ae2364d619c380ad3dfce73ce2020-04-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSystems.00107-20https://doaj.org/toc/2379-5077ABSTRACT Vitamin B12 is synthesized by prokaryotes in the rumens of dairy cows—and this has implications in human nutrition since humans rely on consumption of dairy products for vitamin B12 acquisition. However, the concentration of vitamin B12 in milk is highly variable, and there is interest in determining what causes vitamin B12 variability. We collected 92 temporally linked rumen, fecal, blood, and milk sample sets from Holstein cows at various stages of lactation fitted with rumen cannula and attempted to define which bacterial genera correlated well with vitamin B12 abundance. The level of vitamin B12 present in each sample was measured, and the bacterial population of each rumen, fecal, and milk sample (n = 263) was analyzed by 16S rRNA-targeted amplicon sequencing of the V4 region. The bacterial populations present in the rumen, small intestine, and milk were highly dissimilar. Combined diet and lactation status had significant effects on the composition of the microbiota in the rumen and in the feces. A high ruminal concentration of vitamin B12 was correlated with the increased abundance of Prevotella, while a low ruminal concentration of vitamin B12 was correlated with increased abundance of Bacteroidetes, Ruminiclostridium, and Butyrivibrio. The ultimate concentration of vitamin B12 is controlled by the complex interaction of several factors, including the composition of the microbiota. Bacterial consumption of vitamin B12 in the rumen may be more important in determining overall levels than bacterial production. IMPORTANCE In this paper, we examined the microbiome of the bovine rumen, feces, and milk and attempted to understand how the bacterial communities at each site affected the production and movement of vitamin B12 around the animal’s body. It was determined that the composition of the bovine rumen microbiome correlates well with vitamin B12 concentration, indicating that the rumen microbiota may be a good target for manipulation to improve production of this important vitamin.Julian Franco-LopezMelissa DuplessisAn BuiCoralie ReymondWilliam PoissonLya BlaisJasmine ChongRachel GervaisDaniel E. RicoRoger I. CueChristiane L. GirardJennifer RonholmAmerican Society for Microbiologyarticlebovinemicrobiomemicrobiotamilkrumenvitamin B12MicrobiologyQR1-502ENmSystems, Vol 5, Iss 2 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	bovine microbiome microbiota milk rumen vitamin B12 Microbiology QR1-502
spellingShingle	bovine microbiome microbiota milk rumen vitamin B12 Microbiology QR1-502 Julian Franco-Lopez Melissa Duplessis An Bui Coralie Reymond William Poisson Lya Blais Jasmine Chong Rachel Gervais Daniel E. Rico Roger I. Cue Christiane L. Girard Jennifer Ronholm Correlations between the Composition of the Bovine Microbiota and Vitamin B<sub>12</sub> Abundance
description	ABSTRACT Vitamin B12 is synthesized by prokaryotes in the rumens of dairy cows—and this has implications in human nutrition since humans rely on consumption of dairy products for vitamin B12 acquisition. However, the concentration of vitamin B12 in milk is highly variable, and there is interest in determining what causes vitamin B12 variability. We collected 92 temporally linked rumen, fecal, blood, and milk sample sets from Holstein cows at various stages of lactation fitted with rumen cannula and attempted to define which bacterial genera correlated well with vitamin B12 abundance. The level of vitamin B12 present in each sample was measured, and the bacterial population of each rumen, fecal, and milk sample (n = 263) was analyzed by 16S rRNA-targeted amplicon sequencing of the V4 region. The bacterial populations present in the rumen, small intestine, and milk were highly dissimilar. Combined diet and lactation status had significant effects on the composition of the microbiota in the rumen and in the feces. A high ruminal concentration of vitamin B12 was correlated with the increased abundance of Prevotella, while a low ruminal concentration of vitamin B12 was correlated with increased abundance of Bacteroidetes, Ruminiclostridium, and Butyrivibrio. The ultimate concentration of vitamin B12 is controlled by the complex interaction of several factors, including the composition of the microbiota. Bacterial consumption of vitamin B12 in the rumen may be more important in determining overall levels than bacterial production. IMPORTANCE In this paper, we examined the microbiome of the bovine rumen, feces, and milk and attempted to understand how the bacterial communities at each site affected the production and movement of vitamin B12 around the animal’s body. It was determined that the composition of the bovine rumen microbiome correlates well with vitamin B12 concentration, indicating that the rumen microbiota may be a good target for manipulation to improve production of this important vitamin.
format	article
author	Julian Franco-Lopez Melissa Duplessis An Bui Coralie Reymond William Poisson Lya Blais Jasmine Chong Rachel Gervais Daniel E. Rico Roger I. Cue Christiane L. Girard Jennifer Ronholm
author_facet	Julian Franco-Lopez Melissa Duplessis An Bui Coralie Reymond William Poisson Lya Blais Jasmine Chong Rachel Gervais Daniel E. Rico Roger I. Cue Christiane L. Girard Jennifer Ronholm
author_sort	Julian Franco-Lopez
title	Correlations between the Composition of the Bovine Microbiota and Vitamin B<sub>12</sub> Abundance
title_short	Correlations between the Composition of the Bovine Microbiota and Vitamin B<sub>12</sub> Abundance
title_full	Correlations between the Composition of the Bovine Microbiota and Vitamin B<sub>12</sub> Abundance
title_fullStr	Correlations between the Composition of the Bovine Microbiota and Vitamin B<sub>12</sub> Abundance
title_full_unstemmed	Correlations between the Composition of the Bovine Microbiota and Vitamin B<sub>12</sub> Abundance
title_sort	correlations between the composition of the bovine microbiota and vitamin b<sub>12</sub> abundance
publisher	American Society for Microbiology
publishDate	2020
url	https://doaj.org/article/6d210c2ae2364d619c380ad3dfce73ce
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Correlations between the Composition of the Bovine Microbiota and Vitamin B<sub>12</sub> Abundance

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