Identification of proteins associated with bast fiber growth of ramie by differential proteomic analysis

Abstract Background Ramie is an important fiber-producing crop in China, and its fibers are widely used as textile materials. Fibers contain specialized secondary cellular walls that are mainly composed of cellulose, hemicelluloses, and lignin. Understanding the mechanism underlying the secondary wa...

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Autores principales:	Fu Li, Zheng Zeng, Renyan Huang, Yanzhou Wang, Touming Liu
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Ramie fiber growth Secondary wall biosynthesis Proteome Differential expression Biotechnology TP248.13-248.65 Genetics QH426-470
Acceso en línea:	https://doaj.org/article/6d2cf74af4aa43ba86c214586ea7b094
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spelling	oai:doaj.org-article:6d2cf74af4aa43ba86c214586ea7b0942021-12-05T12:17:20ZIdentification of proteins associated with bast fiber growth of ramie by differential proteomic analysis10.1186/s12864-021-08195-91471-2164https://doaj.org/article/6d2cf74af4aa43ba86c214586ea7b0942021-12-01T00:00:00Zhttps://doi.org/10.1186/s12864-021-08195-9https://doaj.org/toc/1471-2164Abstract Background Ramie is an important fiber-producing crop in China, and its fibers are widely used as textile materials. Fibers contain specialized secondary cellular walls that are mainly composed of cellulose, hemicelluloses, and lignin. Understanding the mechanism underlying the secondary wall biosynthesis of fibers will benefit the improvement of fiber yield and quality in ramie. Results Here, we performed a proteomic analysis of the bark from the top and middle parts of the stem, where fiber growth is at different stages. We identified 6971 non-redundant proteins from bast bark. Proteomic comparison revealed 983 proteins with differential expression between the two bark types. Of these 983 proteins, 46 were identified as the homolog of known secondary wall biosynthetic proteins of Arabidopsis, indicating that they were potentially associated with fiber growth. Then, we proposed a molecular model for the secondary wall biosynthesis of ramie fiber. Furthermore, interaction analysis of 46 candidate proteins revealed two interacting networks that consisted of eight cellulose biosynthetic enzymes and seven lignin biosynthetic proteins, respectively. Conclusion This study sheds light on the proteomic basis underlying bast fiber growth in ramie, and the identification of many candidates associated with fiber growth provides important basis for understanding the fiber growth in this crop.Fu LiZheng ZengRenyan HuangYanzhou WangTouming LiuBMCarticleRamiefiber growthSecondary wall biosynthesisProteomeDifferential expressionBiotechnologyTP248.13-248.65GeneticsQH426-470ENBMC Genomics, Vol 22, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Ramie fiber growth Secondary wall biosynthesis Proteome Differential expression Biotechnology TP248.13-248.65 Genetics QH426-470
spellingShingle	Ramie fiber growth Secondary wall biosynthesis Proteome Differential expression Biotechnology TP248.13-248.65 Genetics QH426-470 Fu Li Zheng Zeng Renyan Huang Yanzhou Wang Touming Liu Identification of proteins associated with bast fiber growth of ramie by differential proteomic analysis
description	Abstract Background Ramie is an important fiber-producing crop in China, and its fibers are widely used as textile materials. Fibers contain specialized secondary cellular walls that are mainly composed of cellulose, hemicelluloses, and lignin. Understanding the mechanism underlying the secondary wall biosynthesis of fibers will benefit the improvement of fiber yield and quality in ramie. Results Here, we performed a proteomic analysis of the bark from the top and middle parts of the stem, where fiber growth is at different stages. We identified 6971 non-redundant proteins from bast bark. Proteomic comparison revealed 983 proteins with differential expression between the two bark types. Of these 983 proteins, 46 were identified as the homolog of known secondary wall biosynthetic proteins of Arabidopsis, indicating that they were potentially associated with fiber growth. Then, we proposed a molecular model for the secondary wall biosynthesis of ramie fiber. Furthermore, interaction analysis of 46 candidate proteins revealed two interacting networks that consisted of eight cellulose biosynthetic enzymes and seven lignin biosynthetic proteins, respectively. Conclusion This study sheds light on the proteomic basis underlying bast fiber growth in ramie, and the identification of many candidates associated with fiber growth provides important basis for understanding the fiber growth in this crop.
format	article
author	Fu Li Zheng Zeng Renyan Huang Yanzhou Wang Touming Liu
author_facet	Fu Li Zheng Zeng Renyan Huang Yanzhou Wang Touming Liu
author_sort	Fu Li
title	Identification of proteins associated with bast fiber growth of ramie by differential proteomic analysis
title_short	Identification of proteins associated with bast fiber growth of ramie by differential proteomic analysis
title_full	Identification of proteins associated with bast fiber growth of ramie by differential proteomic analysis
title_fullStr	Identification of proteins associated with bast fiber growth of ramie by differential proteomic analysis
title_full_unstemmed	Identification of proteins associated with bast fiber growth of ramie by differential proteomic analysis
title_sort	identification of proteins associated with bast fiber growth of ramie by differential proteomic analysis
publisher	BMC
publishDate	2021
url	https://doaj.org/article/6d2cf74af4aa43ba86c214586ea7b094
work_keys_str_mv	AT fuli identificationofproteinsassociatedwithbastfibergrowthoframiebydifferentialproteomicanalysis AT zhengzeng identificationofproteinsassociatedwithbastfibergrowthoframiebydifferentialproteomicanalysis AT renyanhuang identificationofproteinsassociatedwithbastfibergrowthoframiebydifferentialproteomicanalysis AT yanzhouwang identificationofproteinsassociatedwithbastfibergrowthoframiebydifferentialproteomicanalysis AT toumingliu identificationofproteinsassociatedwithbastfibergrowthoframiebydifferentialproteomicanalysis
_version_	1718372078198980608

Identification of proteins associated with bast fiber growth of ramie by differential proteomic analysis

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