Imaging of angiogenesis of human umbilical vein endothelial cells by uptake of exosomes secreted from hepatocellular carcinoma cells

Abstract Hepatocellular carcinoma (HCC) is a typical hyper-vascular tumor, so the understanding the mechanisms of angiogenesis in HCC is very important for its treatment. However, the influence of the exosomes secreted from HCC cells (HCC-exosomes) on angiogenesis remains poorly understood. We herei...

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Autores principales:	Hiroshi Yukawa, Kaoru Suzuki, Keita Aoki, Tomoko Arimoto, Takao Yasui, Noritada Kaji, Tetsuya Ishikawa, Takahiro Ochiya, Yoshinobu Baba
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6d395b856090459d92f4ffcba9b96c91
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spelling	oai:doaj.org-article:6d395b856090459d92f4ffcba9b96c912021-12-02T11:40:17ZImaging of angiogenesis of human umbilical vein endothelial cells by uptake of exosomes secreted from hepatocellular carcinoma cells10.1038/s41598-018-24563-02045-2322https://doaj.org/article/6d395b856090459d92f4ffcba9b96c912018-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-24563-0https://doaj.org/toc/2045-2322Abstract Hepatocellular carcinoma (HCC) is a typical hyper-vascular tumor, so the understanding the mechanisms of angiogenesis in HCC is very important for its treatment. However, the influence of the exosomes secreted from HCC cells (HCC-exosomes) on angiogenesis remains poorly understood. We herein examined the effects of the exosomes secreted from HepG2 cells (HepG2-exosomes) on the lumen formation of human umbilical vein endothelial cells (HUVECs) by the imaging of angiogenesis. The degree of lumen formation of HUVECs was dependent on the number of HepG2-exosomes. The HepG2-exosomes expressed NKG2D, an activating receptor for immune cells, and HSP70, a stress-induced heat shock protein associated with angiogenesis through the vascular endothelial growth factor (VEGF) receptor. In addition, the HepG2-exosomes contained several microRNAs (miRNAs) reported to exist in the serum of HCC patients. These results suggest that the HCC-exosomes play an important role in angiogenesis. Further studies on the function of HCC-exosomes may provide a new target for HCC treatment.Hiroshi YukawaKaoru SuzukiKeita AokiTomoko ArimotoTakao YasuiNoritada KajiTetsuya IshikawaTakahiro OchiyaYoshinobu BabaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-12 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hiroshi Yukawa Kaoru Suzuki Keita Aoki Tomoko Arimoto Takao Yasui Noritada Kaji Tetsuya Ishikawa Takahiro Ochiya Yoshinobu Baba Imaging of angiogenesis of human umbilical vein endothelial cells by uptake of exosomes secreted from hepatocellular carcinoma cells
description	Abstract Hepatocellular carcinoma (HCC) is a typical hyper-vascular tumor, so the understanding the mechanisms of angiogenesis in HCC is very important for its treatment. However, the influence of the exosomes secreted from HCC cells (HCC-exosomes) on angiogenesis remains poorly understood. We herein examined the effects of the exosomes secreted from HepG2 cells (HepG2-exosomes) on the lumen formation of human umbilical vein endothelial cells (HUVECs) by the imaging of angiogenesis. The degree of lumen formation of HUVECs was dependent on the number of HepG2-exosomes. The HepG2-exosomes expressed NKG2D, an activating receptor for immune cells, and HSP70, a stress-induced heat shock protein associated with angiogenesis through the vascular endothelial growth factor (VEGF) receptor. In addition, the HepG2-exosomes contained several microRNAs (miRNAs) reported to exist in the serum of HCC patients. These results suggest that the HCC-exosomes play an important role in angiogenesis. Further studies on the function of HCC-exosomes may provide a new target for HCC treatment.
format	article
author	Hiroshi Yukawa Kaoru Suzuki Keita Aoki Tomoko Arimoto Takao Yasui Noritada Kaji Tetsuya Ishikawa Takahiro Ochiya Yoshinobu Baba
author_facet	Hiroshi Yukawa Kaoru Suzuki Keita Aoki Tomoko Arimoto Takao Yasui Noritada Kaji Tetsuya Ishikawa Takahiro Ochiya Yoshinobu Baba
author_sort	Hiroshi Yukawa
title	Imaging of angiogenesis of human umbilical vein endothelial cells by uptake of exosomes secreted from hepatocellular carcinoma cells
title_short	Imaging of angiogenesis of human umbilical vein endothelial cells by uptake of exosomes secreted from hepatocellular carcinoma cells
title_full	Imaging of angiogenesis of human umbilical vein endothelial cells by uptake of exosomes secreted from hepatocellular carcinoma cells
title_fullStr	Imaging of angiogenesis of human umbilical vein endothelial cells by uptake of exosomes secreted from hepatocellular carcinoma cells
title_full_unstemmed	Imaging of angiogenesis of human umbilical vein endothelial cells by uptake of exosomes secreted from hepatocellular carcinoma cells
title_sort	imaging of angiogenesis of human umbilical vein endothelial cells by uptake of exosomes secreted from hepatocellular carcinoma cells
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/6d395b856090459d92f4ffcba9b96c91
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Imaging of angiogenesis of human umbilical vein endothelial cells by uptake of exosomes secreted from hepatocellular carcinoma cells

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