Aminopropyltriethoxysilane-mediated surface functionalization of hydroxyapatite nanoparticles: synthesis, characterization, and in vitro toxicity assay

Shige Wang1, Shihui Wen2, Mingwu Shen2, Rui Guo2, Xueyan Cao2, Jianhua Wang3, Xiangyang Shi1,2,41State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, 2College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, 3Department of Biochemistry and Molecula...

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Autores principales:	Wang S, Wen S, Shen M, Guo R, Cao X, Wang J, Shi X
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2011
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/6d3f175ee19c4f3c9ee47238380cf63e
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spelling	oai:doaj.org-article:6d3f175ee19c4f3c9ee47238380cf63e2021-12-02T08:08:31ZAminopropyltriethoxysilane-mediated surface functionalization of hydroxyapatite nanoparticles: synthesis, characterization, and in vitro toxicity assay1176-91141178-2013https://doaj.org/article/6d3f175ee19c4f3c9ee47238380cf63e2011-12-01T00:00:00Zhttp://www.dovepress.com/aminopropyltriethoxysilane-mediated-surface-functionalization-of-hydro-a8924https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Shige Wang1, Shihui Wen2, Mingwu Shen2, Rui Guo2, Xueyan Cao2, Jianhua Wang3, Xiangyang Shi1,2,41State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, 2College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, 3Department of Biochemistry and Molecular Cell Biology, School of Medicine, Shanghai Jiao Tong University, Shanghai, People&#39;s Republic of China; 4Centro de Qu&iacute;mica da Madeira, Universidade da Madeira, Campus da Penteada, Funchal, PortugalBackground: We report on aminopropyltriethoxysilane (APTS)-mediated surface modification of nanohydroxyapatite with different surface functional groups for potential biomedical applications. In this study, nanohydroxyapatite covalently linked with APTS (n-HA-APTS) was reacted with acetic anhydride or succinic anhydride to produce neutralized (n-HA-APTS.Ac) or negatively charged (n-HA-APTS.SAH) nanohydroxyapatite, respectively. Nanohydroxyapatite formed with amine, acetyl, and carboxyl groups was extensively characterized using Fourier transform infrared spectroscopy, transmission electron microscopy, 1H nuclear magnetic resonance spectroscopy, X-ray diffraction, inductively coupled plasma-atomic emission spectroscopy, and zeta potential measurements.Results: In vitro 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide colorimetric assay revealed that the slight toxicity of the amine-functionalized n-HA-APTS could be eliminated by post-functionalization of APTS amines to form acetyl and carboxyl groups. Blood compatibility assessment demonstrated that the negligible hemolytic activity of the pristine nanohydroxyapatite particles did not appreciably change after APTS-mediated surface functionalization.Conclusion: APTS-mediated functionalization of nanohydroxyapatite with different surface groups may be useful for further functionalization of nanohydroxyapatite with biologically active materials, thereby providing possibilities for a broad range of biomedical applications.Keywords: aminopropyltriethoxysilane, hydroxyapatite nanoparticles, surface functionalization, acetylation, carboxylation, toxicityWang SWen SShen MGuo RCao XWang JShi XDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2011, Iss default, Pp 3449-3459 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Wang S Wen S Shen M Guo R Cao X Wang J Shi X Aminopropyltriethoxysilane-mediated surface functionalization of hydroxyapatite nanoparticles: synthesis, characterization, and in vitro toxicity assay
description	Shige Wang1, Shihui Wen2, Mingwu Shen2, Rui Guo2, Xueyan Cao2, Jianhua Wang3, Xiangyang Shi1,2,41State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, 2College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, 3Department of Biochemistry and Molecular Cell Biology, School of Medicine, Shanghai Jiao Tong University, Shanghai, People&#39;s Republic of China; 4Centro de Qu&iacute;mica da Madeira, Universidade da Madeira, Campus da Penteada, Funchal, PortugalBackground: We report on aminopropyltriethoxysilane (APTS)-mediated surface modification of nanohydroxyapatite with different surface functional groups for potential biomedical applications. In this study, nanohydroxyapatite covalently linked with APTS (n-HA-APTS) was reacted with acetic anhydride or succinic anhydride to produce neutralized (n-HA-APTS.Ac) or negatively charged (n-HA-APTS.SAH) nanohydroxyapatite, respectively. Nanohydroxyapatite formed with amine, acetyl, and carboxyl groups was extensively characterized using Fourier transform infrared spectroscopy, transmission electron microscopy, 1H nuclear magnetic resonance spectroscopy, X-ray diffraction, inductively coupled plasma-atomic emission spectroscopy, and zeta potential measurements.Results: In vitro 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide colorimetric assay revealed that the slight toxicity of the amine-functionalized n-HA-APTS could be eliminated by post-functionalization of APTS amines to form acetyl and carboxyl groups. Blood compatibility assessment demonstrated that the negligible hemolytic activity of the pristine nanohydroxyapatite particles did not appreciably change after APTS-mediated surface functionalization.Conclusion: APTS-mediated functionalization of nanohydroxyapatite with different surface groups may be useful for further functionalization of nanohydroxyapatite with biologically active materials, thereby providing possibilities for a broad range of biomedical applications.Keywords: aminopropyltriethoxysilane, hydroxyapatite nanoparticles, surface functionalization, acetylation, carboxylation, toxicity
format	article
author	Wang S Wen S Shen M Guo R Cao X Wang J Shi X
author_facet	Wang S Wen S Shen M Guo R Cao X Wang J Shi X
author_sort	Wang S
title	Aminopropyltriethoxysilane-mediated surface functionalization of hydroxyapatite nanoparticles: synthesis, characterization, and in vitro toxicity assay
title_short	Aminopropyltriethoxysilane-mediated surface functionalization of hydroxyapatite nanoparticles: synthesis, characterization, and in vitro toxicity assay
title_full	Aminopropyltriethoxysilane-mediated surface functionalization of hydroxyapatite nanoparticles: synthesis, characterization, and in vitro toxicity assay
title_fullStr	Aminopropyltriethoxysilane-mediated surface functionalization of hydroxyapatite nanoparticles: synthesis, characterization, and in vitro toxicity assay
title_full_unstemmed	Aminopropyltriethoxysilane-mediated surface functionalization of hydroxyapatite nanoparticles: synthesis, characterization, and in vitro toxicity assay
title_sort	aminopropyltriethoxysilane-mediated surface functionalization of hydroxyapatite nanoparticles: synthesis, characterization, and in vitro toxicity assay
publisher	Dove Medical Press
publishDate	2011
url	https://doaj.org/article/6d3f175ee19c4f3c9ee47238380cf63e
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Aminopropyltriethoxysilane-mediated surface functionalization of hydroxyapatite nanoparticles: synthesis, characterization, and in vitro toxicity assay

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