In vivo genotoxicity assessment of titanium dioxide nanoparticles by Allium cepa root tip assay at high exposure concentrations.

The industrial production and commercial applications of titanium dioxide nanoparticles have increased considerably in recent times, which has increased the probability of environmental contamination with these agents and their adverse effects on living systems. This study was designed to assess the...

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Autores principales:	Sunandan Pakrashi, Nitin Jain, Swayamprava Dalai, Jerobin Jayakumar, Prathna Thanjavur Chandrasekaran, Ashok M Raichur, Natarajan Chandrasekaran, Amitava Mukherjee
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6d41794d89c6431a94bd91f6385d6275
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spelling	oai:doaj.org-article:6d41794d89c6431a94bd91f6385d62752021-11-18T08:33:57ZIn vivo genotoxicity assessment of titanium dioxide nanoparticles by Allium cepa root tip assay at high exposure concentrations.1932-620310.1371/journal.pone.0087789https://doaj.org/article/6d41794d89c6431a94bd91f6385d62752014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24504252/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The industrial production and commercial applications of titanium dioxide nanoparticles have increased considerably in recent times, which has increased the probability of environmental contamination with these agents and their adverse effects on living systems. This study was designed to assess the genotoxicity potential of TiO2 NPs at high exposure concentrations, its bio-uptake, and the oxidative stress it generated, a recognised cause of genotoxicity. Allium cepa root tips were treated with TiO2 NP dispersions at four different concentrations (12.5, 25, 50, 100 µg/mL). A dose dependant decrease in the mitotic index (69 to 21) and an increase in the number of distinctive chromosomal aberrations were observed. Optical, fluorescence and confocal laser scanning microscopy revealed chromosomal aberrations, including chromosomal breaks and sticky, multipolar, and laggard chromosomes, and micronucleus formation. The chromosomal aberrations and DNA damage were also validated by the comet assay. The bio-uptake of TiO2 in particulate form was the key cause of reactive oxygen species generation, which in turn was probably the cause of the DNA aberrations and genotoxicity observed in this study.Sunandan PakrashiNitin JainSwayamprava DalaiJerobin JayakumarPrathna Thanjavur ChandrasekaranAshok M RaichurNatarajan ChandrasekaranAmitava MukherjeePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 2, p e87789 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sunandan Pakrashi Nitin Jain Swayamprava Dalai Jerobin Jayakumar Prathna Thanjavur Chandrasekaran Ashok M Raichur Natarajan Chandrasekaran Amitava Mukherjee In vivo genotoxicity assessment of titanium dioxide nanoparticles by Allium cepa root tip assay at high exposure concentrations.
description	The industrial production and commercial applications of titanium dioxide nanoparticles have increased considerably in recent times, which has increased the probability of environmental contamination with these agents and their adverse effects on living systems. This study was designed to assess the genotoxicity potential of TiO2 NPs at high exposure concentrations, its bio-uptake, and the oxidative stress it generated, a recognised cause of genotoxicity. Allium cepa root tips were treated with TiO2 NP dispersions at four different concentrations (12.5, 25, 50, 100 µg/mL). A dose dependant decrease in the mitotic index (69 to 21) and an increase in the number of distinctive chromosomal aberrations were observed. Optical, fluorescence and confocal laser scanning microscopy revealed chromosomal aberrations, including chromosomal breaks and sticky, multipolar, and laggard chromosomes, and micronucleus formation. The chromosomal aberrations and DNA damage were also validated by the comet assay. The bio-uptake of TiO2 in particulate form was the key cause of reactive oxygen species generation, which in turn was probably the cause of the DNA aberrations and genotoxicity observed in this study.
format	article
author	Sunandan Pakrashi Nitin Jain Swayamprava Dalai Jerobin Jayakumar Prathna Thanjavur Chandrasekaran Ashok M Raichur Natarajan Chandrasekaran Amitava Mukherjee
author_facet	Sunandan Pakrashi Nitin Jain Swayamprava Dalai Jerobin Jayakumar Prathna Thanjavur Chandrasekaran Ashok M Raichur Natarajan Chandrasekaran Amitava Mukherjee
author_sort	Sunandan Pakrashi
title	In vivo genotoxicity assessment of titanium dioxide nanoparticles by Allium cepa root tip assay at high exposure concentrations.
title_short	In vivo genotoxicity assessment of titanium dioxide nanoparticles by Allium cepa root tip assay at high exposure concentrations.
title_full	In vivo genotoxicity assessment of titanium dioxide nanoparticles by Allium cepa root tip assay at high exposure concentrations.
title_fullStr	In vivo genotoxicity assessment of titanium dioxide nanoparticles by Allium cepa root tip assay at high exposure concentrations.
title_full_unstemmed	In vivo genotoxicity assessment of titanium dioxide nanoparticles by Allium cepa root tip assay at high exposure concentrations.
title_sort	in vivo genotoxicity assessment of titanium dioxide nanoparticles by allium cepa root tip assay at high exposure concentrations.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/6d41794d89c6431a94bd91f6385d6275
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In vivo genotoxicity assessment of titanium dioxide nanoparticles by Allium cepa root tip assay at high exposure concentrations.

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