The Elite Alleles of OsSPL4 Regulate Grain Size and Increase Grain Yield in Rice

Abstract Grain weight and grain number, the two important yield traits, are mainly determined by grain size and panicle architecture in rice. Herein, we report the identification and functional analysis of OsSPL4 in panicle and grain development of rice. Using CRISPR/Cas9 system, two elite alleles o...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jihong Hu, Liyu Huang, Guanglong Chen, Hui Liu, Yesheng Zhang, Ru Zhang, Shilai Zhang, Jintao Liu, Qingyi Hu, Fengyi Hu, Wen Wang, Yi Ding
Formato:	article
Lenguaje:	EN
Publicado:	SpringerOpen 2021
Materias:	OsSPL4 Grain size Grain weight Panicle branching Grain yield Rice Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/6d4de70d73854accb0254ee9a9d674b5
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:6d4de70d73854accb0254ee9a9d674b5
record_format	dspace
spelling	oai:doaj.org-article:6d4de70d73854accb0254ee9a9d674b52021-11-08T11:15:19ZThe Elite Alleles of OsSPL4 Regulate Grain Size and Increase Grain Yield in Rice10.1186/s12284-021-00531-71939-84251939-8433https://doaj.org/article/6d4de70d73854accb0254ee9a9d674b52021-11-01T00:00:00Zhttps://doi.org/10.1186/s12284-021-00531-7https://doaj.org/toc/1939-8425https://doaj.org/toc/1939-8433Abstract Grain weight and grain number, the two important yield traits, are mainly determined by grain size and panicle architecture in rice. Herein, we report the identification and functional analysis of OsSPL4 in panicle and grain development of rice. Using CRISPR/Cas9 system, two elite alleles of OsSPL4 were obtained, which exhibited an increasing number of grains per panicle and grain size, resulting in increase of rice yield. Cytological analysis showed that OsSPL4 could regulate spikelet development by promoting cell division. The results of RNA-seq and qRT-PCR validations also demonstrated that several MADS-box and cell-cycle genes were up-regulated in the mutation lines. Co-expression network revealed that many yield-related genes were involved in the regulation network of OsSPL4. In addition, OsSPL4 could be cleaved by the osa-miR156 in vivo, and the OsmiR156-OsSPL4 module might regulate the grain size in rice. Further analysis indicated that the large-grain allele of OsSPL4 in indica rice might introgress from aus varieties under artificial selection. Taken together, our findings suggested that OsSPL4 could be as a key regulator of grain size by acting on cell division control and provided a strategy for panicle architecture and grain size modification for yield improvement in rice.Jihong HuLiyu HuangGuanglong ChenHui LiuYesheng ZhangRu ZhangShilai ZhangJintao LiuQingyi HuFengyi HuWen WangYi DingSpringerOpenarticleOsSPL4Grain sizeGrain weightPanicle branchingGrain yieldRicePlant cultureSB1-1110ENRice, Vol 14, Iss 1, Pp 1-18 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	OsSPL4 Grain size Grain weight Panicle branching Grain yield Rice Plant culture SB1-1110
spellingShingle	OsSPL4 Grain size Grain weight Panicle branching Grain yield Rice Plant culture SB1-1110 Jihong Hu Liyu Huang Guanglong Chen Hui Liu Yesheng Zhang Ru Zhang Shilai Zhang Jintao Liu Qingyi Hu Fengyi Hu Wen Wang Yi Ding The Elite Alleles of OsSPL4 Regulate Grain Size and Increase Grain Yield in Rice
description	Abstract Grain weight and grain number, the two important yield traits, are mainly determined by grain size and panicle architecture in rice. Herein, we report the identification and functional analysis of OsSPL4 in panicle and grain development of rice. Using CRISPR/Cas9 system, two elite alleles of OsSPL4 were obtained, which exhibited an increasing number of grains per panicle and grain size, resulting in increase of rice yield. Cytological analysis showed that OsSPL4 could regulate spikelet development by promoting cell division. The results of RNA-seq and qRT-PCR validations also demonstrated that several MADS-box and cell-cycle genes were up-regulated in the mutation lines. Co-expression network revealed that many yield-related genes were involved in the regulation network of OsSPL4. In addition, OsSPL4 could be cleaved by the osa-miR156 in vivo, and the OsmiR156-OsSPL4 module might regulate the grain size in rice. Further analysis indicated that the large-grain allele of OsSPL4 in indica rice might introgress from aus varieties under artificial selection. Taken together, our findings suggested that OsSPL4 could be as a key regulator of grain size by acting on cell division control and provided a strategy for panicle architecture and grain size modification for yield improvement in rice.
format	article
author	Jihong Hu Liyu Huang Guanglong Chen Hui Liu Yesheng Zhang Ru Zhang Shilai Zhang Jintao Liu Qingyi Hu Fengyi Hu Wen Wang Yi Ding
author_facet	Jihong Hu Liyu Huang Guanglong Chen Hui Liu Yesheng Zhang Ru Zhang Shilai Zhang Jintao Liu Qingyi Hu Fengyi Hu Wen Wang Yi Ding
author_sort	Jihong Hu
title	The Elite Alleles of OsSPL4 Regulate Grain Size and Increase Grain Yield in Rice
title_short	The Elite Alleles of OsSPL4 Regulate Grain Size and Increase Grain Yield in Rice
title_full	The Elite Alleles of OsSPL4 Regulate Grain Size and Increase Grain Yield in Rice
title_fullStr	The Elite Alleles of OsSPL4 Regulate Grain Size and Increase Grain Yield in Rice
title_full_unstemmed	The Elite Alleles of OsSPL4 Regulate Grain Size and Increase Grain Yield in Rice
title_sort	elite alleles of osspl4 regulate grain size and increase grain yield in rice
publisher	SpringerOpen
publishDate	2021
url	https://doaj.org/article/6d4de70d73854accb0254ee9a9d674b5
work_keys_str_mv	AT jihonghu theeliteallelesofosspl4regulategrainsizeandincreasegrainyieldinrice AT liyuhuang theeliteallelesofosspl4regulategrainsizeandincreasegrainyieldinrice AT guanglongchen theeliteallelesofosspl4regulategrainsizeandincreasegrainyieldinrice AT huiliu theeliteallelesofosspl4regulategrainsizeandincreasegrainyieldinrice AT yeshengzhang theeliteallelesofosspl4regulategrainsizeandincreasegrainyieldinrice AT ruzhang theeliteallelesofosspl4regulategrainsizeandincreasegrainyieldinrice AT shilaizhang theeliteallelesofosspl4regulategrainsizeandincreasegrainyieldinrice AT jintaoliu theeliteallelesofosspl4regulategrainsizeandincreasegrainyieldinrice AT qingyihu theeliteallelesofosspl4regulategrainsizeandincreasegrainyieldinrice AT fengyihu theeliteallelesofosspl4regulategrainsizeandincreasegrainyieldinrice AT wenwang theeliteallelesofosspl4regulategrainsizeandincreasegrainyieldinrice AT yiding theeliteallelesofosspl4regulategrainsizeandincreasegrainyieldinrice AT jihonghu eliteallelesofosspl4regulategrainsizeandincreasegrainyieldinrice AT liyuhuang eliteallelesofosspl4regulategrainsizeandincreasegrainyieldinrice AT guanglongchen eliteallelesofosspl4regulategrainsizeandincreasegrainyieldinrice AT huiliu eliteallelesofosspl4regulategrainsizeandincreasegrainyieldinrice AT yeshengzhang eliteallelesofosspl4regulategrainsizeandincreasegrainyieldinrice AT ruzhang eliteallelesofosspl4regulategrainsizeandincreasegrainyieldinrice AT shilaizhang eliteallelesofosspl4regulategrainsizeandincreasegrainyieldinrice AT jintaoliu eliteallelesofosspl4regulategrainsizeandincreasegrainyieldinrice AT qingyihu eliteallelesofosspl4regulategrainsizeandincreasegrainyieldinrice AT fengyihu eliteallelesofosspl4regulategrainsizeandincreasegrainyieldinrice AT wenwang eliteallelesofosspl4regulategrainsizeandincreasegrainyieldinrice AT yiding eliteallelesofosspl4regulategrainsizeandincreasegrainyieldinrice
_version_	1718442342717849600

The Elite Alleles of OsSPL4 Regulate Grain Size and Increase Grain Yield in Rice

Ejemplares similares