The roles of Y-box-binding protein (YB)-1 and C-X-C motif chemokine ligand 14 (CXCL14) in the progression of prostate cancer via extracellular-signal-regulated kinase (ERK) signaling

The cold-shock protein Y-box-binding protein (YB)-1 regulates the expression of various chemokines and their receptors at the transcriptional level. Expression of the orphan chemokine CXCL14 is repressed by EGF induced signaling. The possible links between EGF-mediated YB-1 and CXCL14 as well as the...

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Autores principales:	Chen Wang, Na Zhao, Fuyuki Sato, Keiji Tanimoto, Hiroyuki Okada, Yang Liu, Ujjal K. Bhawal
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	prostate cancer yb-1 cxcl14 egf erk pathway Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/6d521762721743228ab3d51f843e7e46
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spelling	oai:doaj.org-article:6d521762721743228ab3d51f843e7e462021-11-04T15:51:54ZThe roles of Y-box-binding protein (YB)-1 and C-X-C motif chemokine ligand 14 (CXCL14) in the progression of prostate cancer via extracellular-signal-regulated kinase (ERK) signaling2165-59792165-598710.1080/21655979.2021.1993537https://doaj.org/article/6d521762721743228ab3d51f843e7e462021-10-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.1993537https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987The cold-shock protein Y-box-binding protein (YB)-1 regulates the expression of various chemokines and their receptors at the transcriptional level. Expression of the orphan chemokine CXCL14 is repressed by EGF induced signaling. The possible links between EGF-mediated YB-1 and CXCL14 as well as the functions of critical kinase pathways in the progression of prostate cancer have remained unexplored. Here we examined the correlation between YB-1 and CXCL14, and the ERK/AKT/mTOR pathways in prostate cancer. Knockdown of YB-1 decreased cyclinD1 expression with an upregulation of cleaved-PARP in human prostate cancer cells. EGF treatment upregulated phospho-YB-1 expression in a time-dependent manner, while treatment with an ERK inhibitor completely silenced its expression in prostate cancer cells. EGF treatment stimulates CyclinD1 and YB-1 phosphorylation in an ERK-dependent pathway. Positive and negative regulation of YB-1 and CXCL14 was observed after EGF treatment in prostate cancer cells, respectively. EGF rescues cell cycle and apoptosis via the AKT and ERK pathways. Furthermore, YB-1 silencing induces G1 arrest and apoptosis, while knockdown of CXCL14 facilitates cell growth and inhibits apoptosis in prostate cancer cells. YB-1 and CXCL14 were inversely correlated in prostate cancer cells and tissues. A significant association between poor overall survival and High YB-1 expression was observed in human prostate cancer patients. In conclusion, our data reveal the functional relationship between YB-1 and CXCL14 in EGF mediated ERK signaling, and YB-1 expression is a significant prognostic marker to predict prostate cancer.Chen WangNa ZhaoFuyuki SatoKeiji TanimotoHiroyuki OkadaYang LiuUjjal K. BhawalTaylor & Francis Grouparticleprostate canceryb-1cxcl14egferk pathwayBiotechnologyTP248.13-248.65ENBioengineered, Vol 0, Iss 0 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	prostate cancer yb-1 cxcl14 egf erk pathway Biotechnology TP248.13-248.65
spellingShingle	prostate cancer yb-1 cxcl14 egf erk pathway Biotechnology TP248.13-248.65 Chen Wang Na Zhao Fuyuki Sato Keiji Tanimoto Hiroyuki Okada Yang Liu Ujjal K. Bhawal The roles of Y-box-binding protein (YB)-1 and C-X-C motif chemokine ligand 14 (CXCL14) in the progression of prostate cancer via extracellular-signal-regulated kinase (ERK) signaling
description	The cold-shock protein Y-box-binding protein (YB)-1 regulates the expression of various chemokines and their receptors at the transcriptional level. Expression of the orphan chemokine CXCL14 is repressed by EGF induced signaling. The possible links between EGF-mediated YB-1 and CXCL14 as well as the functions of critical kinase pathways in the progression of prostate cancer have remained unexplored. Here we examined the correlation between YB-1 and CXCL14, and the ERK/AKT/mTOR pathways in prostate cancer. Knockdown of YB-1 decreased cyclinD1 expression with an upregulation of cleaved-PARP in human prostate cancer cells. EGF treatment upregulated phospho-YB-1 expression in a time-dependent manner, while treatment with an ERK inhibitor completely silenced its expression in prostate cancer cells. EGF treatment stimulates CyclinD1 and YB-1 phosphorylation in an ERK-dependent pathway. Positive and negative regulation of YB-1 and CXCL14 was observed after EGF treatment in prostate cancer cells, respectively. EGF rescues cell cycle and apoptosis via the AKT and ERK pathways. Furthermore, YB-1 silencing induces G1 arrest and apoptosis, while knockdown of CXCL14 facilitates cell growth and inhibits apoptosis in prostate cancer cells. YB-1 and CXCL14 were inversely correlated in prostate cancer cells and tissues. A significant association between poor overall survival and High YB-1 expression was observed in human prostate cancer patients. In conclusion, our data reveal the functional relationship between YB-1 and CXCL14 in EGF mediated ERK signaling, and YB-1 expression is a significant prognostic marker to predict prostate cancer.
format	article
author	Chen Wang Na Zhao Fuyuki Sato Keiji Tanimoto Hiroyuki Okada Yang Liu Ujjal K. Bhawal
author_facet	Chen Wang Na Zhao Fuyuki Sato Keiji Tanimoto Hiroyuki Okada Yang Liu Ujjal K. Bhawal
author_sort	Chen Wang
title	The roles of Y-box-binding protein (YB)-1 and C-X-C motif chemokine ligand 14 (CXCL14) in the progression of prostate cancer via extracellular-signal-regulated kinase (ERK) signaling
title_short	The roles of Y-box-binding protein (YB)-1 and C-X-C motif chemokine ligand 14 (CXCL14) in the progression of prostate cancer via extracellular-signal-regulated kinase (ERK) signaling
title_full	The roles of Y-box-binding protein (YB)-1 and C-X-C motif chemokine ligand 14 (CXCL14) in the progression of prostate cancer via extracellular-signal-regulated kinase (ERK) signaling
title_fullStr	The roles of Y-box-binding protein (YB)-1 and C-X-C motif chemokine ligand 14 (CXCL14) in the progression of prostate cancer via extracellular-signal-regulated kinase (ERK) signaling
title_full_unstemmed	The roles of Y-box-binding protein (YB)-1 and C-X-C motif chemokine ligand 14 (CXCL14) in the progression of prostate cancer via extracellular-signal-regulated kinase (ERK) signaling
title_sort	roles of y-box-binding protein (yb)-1 and c-x-c motif chemokine ligand 14 (cxcl14) in the progression of prostate cancer via extracellular-signal-regulated kinase (erk) signaling
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/6d521762721743228ab3d51f843e7e46
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The roles of Y-box-binding protein (YB)-1 and C-X-C motif chemokine ligand 14 (CXCL14) in the progression of prostate cancer via extracellular-signal-regulated kinase (ERK) signaling

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