Insights into the functionality of endophytic actinobacteria with a focus on their biosynthetic potential and secondary metabolites production

Abstract Endophytic actinobacteria play an important role in growth promotion and development of host plant by producing enormous quantities of novel bioactive natural products. In the present investigation, 169 endophytic actinobacteria were isolated from endospheric tissues of Rhynchotoechum ellip...

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Autores principales:	Ajit Kumar Passari, Vineet Kumar Mishra, Garima Singh, Pratibha Singh, Brijesh Kumar, Vijai Kumar Gupta, Rupak Kumar Sarma, Ratul Saikia, Anthonia O’. Donovan, Bhim Pratap Singh
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6d5b384e383d4afa9e22938afba24d6b
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spelling	oai:doaj.org-article:6d5b384e383d4afa9e22938afba24d6b2021-12-02T11:52:37ZInsights into the functionality of endophytic actinobacteria with a focus on their biosynthetic potential and secondary metabolites production10.1038/s41598-017-12235-42045-2322https://doaj.org/article/6d5b384e383d4afa9e22938afba24d6b2017-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-12235-4https://doaj.org/toc/2045-2322Abstract Endophytic actinobacteria play an important role in growth promotion and development of host plant by producing enormous quantities of novel bioactive natural products. In the present investigation, 169 endophytic actinobacteria were isolated from endospheric tissues of Rhynchotoechum ellipticum. Based on their antimicrobial potential, 81 strains were identified by 16rRNA gene analysis, which were taxonomically grouped into 15 genera. All identified strains were screened for their plant growth promoting attributes and, for the presence of modular polyketide synthases (PKSI, PKSII and nonribosomal peptide synthetase (NRPS) gene clusters to correlate the biosynthetic genes with their functional properties. Expression studies and antioxidant potential for four representative strains were evaluated using qRT-PCR and DPPH assay respectively. Additionally, six antibiotics (erythromycin, ketoconazole, fluconazole, chloramphenicol, rifampicin and miconazole) and nine phenolic compounds (catechin, kaempferol, chebulagic acid, chlorogenic acid, Asiatic acid, ferulic acid, arjunic acid, gallic acid and boswellic acid) were detected and quantified using UHPLC-QqQLIT-MS/MS. Furthermore, three strains (BPSAC77, 121 and 101) showed the presence of the anticancerous compound paclitaxel which was reported for the first time from endophytic actinobacteria. This study provides a holistic picture, that endophytic actinobacteria are rich bacterial resource for bioactive natural products, which has a great prospective in agriculture and pharmaceutical industries.Ajit Kumar PassariVineet Kumar MishraGarima SinghPratibha SinghBrijesh KumarVijai Kumar GuptaRupak Kumar SarmaRatul SaikiaAnthonia O’. DonovanBhim Pratap SinghNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-17 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ajit Kumar Passari Vineet Kumar Mishra Garima Singh Pratibha Singh Brijesh Kumar Vijai Kumar Gupta Rupak Kumar Sarma Ratul Saikia Anthonia O’. Donovan Bhim Pratap Singh Insights into the functionality of endophytic actinobacteria with a focus on their biosynthetic potential and secondary metabolites production
description	Abstract Endophytic actinobacteria play an important role in growth promotion and development of host plant by producing enormous quantities of novel bioactive natural products. In the present investigation, 169 endophytic actinobacteria were isolated from endospheric tissues of Rhynchotoechum ellipticum. Based on their antimicrobial potential, 81 strains were identified by 16rRNA gene analysis, which were taxonomically grouped into 15 genera. All identified strains were screened for their plant growth promoting attributes and, for the presence of modular polyketide synthases (PKSI, PKSII and nonribosomal peptide synthetase (NRPS) gene clusters to correlate the biosynthetic genes with their functional properties. Expression studies and antioxidant potential for four representative strains were evaluated using qRT-PCR and DPPH assay respectively. Additionally, six antibiotics (erythromycin, ketoconazole, fluconazole, chloramphenicol, rifampicin and miconazole) and nine phenolic compounds (catechin, kaempferol, chebulagic acid, chlorogenic acid, Asiatic acid, ferulic acid, arjunic acid, gallic acid and boswellic acid) were detected and quantified using UHPLC-QqQLIT-MS/MS. Furthermore, three strains (BPSAC77, 121 and 101) showed the presence of the anticancerous compound paclitaxel which was reported for the first time from endophytic actinobacteria. This study provides a holistic picture, that endophytic actinobacteria are rich bacterial resource for bioactive natural products, which has a great prospective in agriculture and pharmaceutical industries.
format	article
author	Ajit Kumar Passari Vineet Kumar Mishra Garima Singh Pratibha Singh Brijesh Kumar Vijai Kumar Gupta Rupak Kumar Sarma Ratul Saikia Anthonia O’. Donovan Bhim Pratap Singh
author_facet	Ajit Kumar Passari Vineet Kumar Mishra Garima Singh Pratibha Singh Brijesh Kumar Vijai Kumar Gupta Rupak Kumar Sarma Ratul Saikia Anthonia O’. Donovan Bhim Pratap Singh
author_sort	Ajit Kumar Passari
title	Insights into the functionality of endophytic actinobacteria with a focus on their biosynthetic potential and secondary metabolites production
title_short	Insights into the functionality of endophytic actinobacteria with a focus on their biosynthetic potential and secondary metabolites production
title_full	Insights into the functionality of endophytic actinobacteria with a focus on their biosynthetic potential and secondary metabolites production
title_fullStr	Insights into the functionality of endophytic actinobacteria with a focus on their biosynthetic potential and secondary metabolites production
title_full_unstemmed	Insights into the functionality of endophytic actinobacteria with a focus on their biosynthetic potential and secondary metabolites production
title_sort	insights into the functionality of endophytic actinobacteria with a focus on their biosynthetic potential and secondary metabolites production
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/6d5b384e383d4afa9e22938afba24d6b
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Insights into the functionality of endophytic actinobacteria with a focus on their biosynthetic potential and secondary metabolites production

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