Identification and characterization of three novel lipases belonging to families II and V from Anaerovibrio lipolyticus 5ST.

Following the isolation, cultivation and characterization of the rumen bacterium Anaerovibrio lipolyticus in the 1960s, it has been recognized as one of the major species involved in lipid hydrolysis in ruminant animals. However, there has been limited characterization of the lipases from the bacter...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Florence Privé, Naheed N Kaderbhai, Susan Girdwood, Hilary J Worgan, Eric Pinloche, Nigel D Scollan, Sharon A Huws, C Jamie Newbold
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6d67226e0eed4e4ab2f3345cbe82e854
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:6d67226e0eed4e4ab2f3345cbe82e854
record_format	dspace
spelling	oai:doaj.org-article:6d67226e0eed4e4ab2f3345cbe82e8542021-11-18T09:00:20ZIdentification and characterization of three novel lipases belonging to families II and V from Anaerovibrio lipolyticus 5ST.1932-620310.1371/journal.pone.0069076https://doaj.org/article/6d67226e0eed4e4ab2f3345cbe82e8542013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23950883/?tool=EBIhttps://doaj.org/toc/1932-6203Following the isolation, cultivation and characterization of the rumen bacterium Anaerovibrio lipolyticus in the 1960s, it has been recognized as one of the major species involved in lipid hydrolysis in ruminant animals. However, there has been limited characterization of the lipases from the bacterium, despite the importance of understanding lipolysis and its impact on subsequent biohydrogenation of polyunsaturated fatty acids by rumen microbes. This study describes the draft genome of Anaerovibrio lipolytica 5ST, and the characterization of three lipolytic genes and their translated protein. The uncompleted draft genome was 2.83 Mbp and comprised of 2,673 coding sequences with a G+C content of 43.3%. Three putative lipase genes, alipA, alipB and alipC, encoding 492-, 438- and 248- amino acid peptides respectively, were identified using RAST. Phylogenetic analysis indicated that alipA and alipB clustered with the GDSL/SGNH family II, and alipC clustered with lipolytic enzymes from family V. Subsequent expression and purification of the enzymes showed that they were thermally unstable and had higher activities at neutral to alkaline pH. Substrate specificity assays indicated that the enzymes had higher hydrolytic activity against caprylate (C8), laurate (C12) and myristate (C14).Florence PrivéNaheed N KaderbhaiSusan GirdwoodHilary J WorganEric PinlocheNigel D ScollanSharon A HuwsC Jamie NewboldPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 8, p e69076 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Florence Privé Naheed N Kaderbhai Susan Girdwood Hilary J Worgan Eric Pinloche Nigel D Scollan Sharon A Huws C Jamie Newbold Identification and characterization of three novel lipases belonging to families II and V from Anaerovibrio lipolyticus 5ST.
description	Following the isolation, cultivation and characterization of the rumen bacterium Anaerovibrio lipolyticus in the 1960s, it has been recognized as one of the major species involved in lipid hydrolysis in ruminant animals. However, there has been limited characterization of the lipases from the bacterium, despite the importance of understanding lipolysis and its impact on subsequent biohydrogenation of polyunsaturated fatty acids by rumen microbes. This study describes the draft genome of Anaerovibrio lipolytica 5ST, and the characterization of three lipolytic genes and their translated protein. The uncompleted draft genome was 2.83 Mbp and comprised of 2,673 coding sequences with a G+C content of 43.3%. Three putative lipase genes, alipA, alipB and alipC, encoding 492-, 438- and 248- amino acid peptides respectively, were identified using RAST. Phylogenetic analysis indicated that alipA and alipB clustered with the GDSL/SGNH family II, and alipC clustered with lipolytic enzymes from family V. Subsequent expression and purification of the enzymes showed that they were thermally unstable and had higher activities at neutral to alkaline pH. Substrate specificity assays indicated that the enzymes had higher hydrolytic activity against caprylate (C8), laurate (C12) and myristate (C14).
format	article
author	Florence Privé Naheed N Kaderbhai Susan Girdwood Hilary J Worgan Eric Pinloche Nigel D Scollan Sharon A Huws C Jamie Newbold
author_facet	Florence Privé Naheed N Kaderbhai Susan Girdwood Hilary J Worgan Eric Pinloche Nigel D Scollan Sharon A Huws C Jamie Newbold
author_sort	Florence Privé
title	Identification and characterization of three novel lipases belonging to families II and V from Anaerovibrio lipolyticus 5ST.
title_short	Identification and characterization of three novel lipases belonging to families II and V from Anaerovibrio lipolyticus 5ST.
title_full	Identification and characterization of three novel lipases belonging to families II and V from Anaerovibrio lipolyticus 5ST.
title_fullStr	Identification and characterization of three novel lipases belonging to families II and V from Anaerovibrio lipolyticus 5ST.
title_full_unstemmed	Identification and characterization of three novel lipases belonging to families II and V from Anaerovibrio lipolyticus 5ST.
title_sort	identification and characterization of three novel lipases belonging to families ii and v from anaerovibrio lipolyticus 5st.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/6d67226e0eed4e4ab2f3345cbe82e854
work_keys_str_mv	AT florenceprive identificationandcharacterizationofthreenovellipasesbelongingtofamiliesiiandvfromanaerovibriolipolyticus5st AT naheednkaderbhai identificationandcharacterizationofthreenovellipasesbelongingtofamiliesiiandvfromanaerovibriolipolyticus5st AT susangirdwood identificationandcharacterizationofthreenovellipasesbelongingtofamiliesiiandvfromanaerovibriolipolyticus5st AT hilaryjworgan identificationandcharacterizationofthreenovellipasesbelongingtofamiliesiiandvfromanaerovibriolipolyticus5st AT ericpinloche identificationandcharacterizationofthreenovellipasesbelongingtofamiliesiiandvfromanaerovibriolipolyticus5st AT nigeldscollan identificationandcharacterizationofthreenovellipasesbelongingtofamiliesiiandvfromanaerovibriolipolyticus5st AT sharonahuws identificationandcharacterizationofthreenovellipasesbelongingtofamiliesiiandvfromanaerovibriolipolyticus5st AT cjamienewbold identificationandcharacterizationofthreenovellipasesbelongingtofamiliesiiandvfromanaerovibriolipolyticus5st
_version_	1718421026114633728

Identification and characterization of three novel lipases belonging to families II and V from Anaerovibrio lipolyticus 5ST.

Ejemplares similares