Study on the bZIP-Type Transcription Factors NapA and RsmA in the Regulation of Intracellular Reactive Species Levels and Sterigmatocystin Production of <i>Aspergillus nidulans</i>

Basic leucine zipper (bZIP) transcription factors play a crucial role in the environmental stress response of eukaryotes. In this work, we studied the effect of gene manipulations, including both deletions and overexpressions, of two selected bZIP transcription factors, NapA and RsmA, in the oxidati...

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Autores principales:	Bernadett Bákány, Wen-Bing Yin, Beatrix Dienes, Tibor Nagy, Éva Leiter, Tamás Emri, Nancy P. Keller, István Pócsi
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Aspergillus nidulans</i> bZIP-type transcription factors oxidative stress secondary metabolite production sterigmatocystin catalase Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/6d6a45e2103740de8f002ceb52796797
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spelling	oai:doaj.org-article:6d6a45e2103740de8f002ceb527967972021-11-11T17:03:37ZStudy on the bZIP-Type Transcription Factors NapA and RsmA in the Regulation of Intracellular Reactive Species Levels and Sterigmatocystin Production of <i>Aspergillus nidulans</i>10.3390/ijms2221115771422-00671661-6596https://doaj.org/article/6d6a45e2103740de8f002ceb527967972021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11577https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Basic leucine zipper (bZIP) transcription factors play a crucial role in the environmental stress response of eukaryotes. In this work, we studied the effect of gene manipulations, including both deletions and overexpressions, of two selected bZIP transcription factors, NapA and RsmA, in the oxidative stress response and sterigmatocystin production of <i>Aspergillus nidulans</i>. We found that NapA was important in the oxidative stress response by negatively regulating intracellular reactive species production and positively regulating catalase activities, whereas RsmA slightly negatively regulated catalase activities. Concerning sterigmatocystin production, the highest concentration was measured in the <i>ΔrsmA</i><i>ΔnapA</i> double deletion mutant, but elevated sterigmatocystin production was also found in the OE<i>rsmA</i> OE<i>napA</i> strain. Our results indicate that NapA influences sterigmatocystin production via regulating reactive species level whereas RsmA modulates toxin production independently of the redox regulation of the cells.Bernadett BákányWen-Bing YinBeatrix DienesTibor NagyÉva LeiterTamás EmriNancy P. KellerIstván PócsiMDPI AGarticle<i>Aspergillus nidulans</i>bZIP-type transcription factorsoxidative stresssecondary metabolite productionsterigmatocystincatalaseBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11577, p 11577 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Aspergillus nidulans</i> bZIP-type transcription factors oxidative stress secondary metabolite production sterigmatocystin catalase Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	<i>Aspergillus nidulans</i> bZIP-type transcription factors oxidative stress secondary metabolite production sterigmatocystin catalase Biology (General) QH301-705.5 Chemistry QD1-999 Bernadett Bákány Wen-Bing Yin Beatrix Dienes Tibor Nagy Éva Leiter Tamás Emri Nancy P. Keller István Pócsi Study on the bZIP-Type Transcription Factors NapA and RsmA in the Regulation of Intracellular Reactive Species Levels and Sterigmatocystin Production of <i>Aspergillus nidulans</i>
description	Basic leucine zipper (bZIP) transcription factors play a crucial role in the environmental stress response of eukaryotes. In this work, we studied the effect of gene manipulations, including both deletions and overexpressions, of two selected bZIP transcription factors, NapA and RsmA, in the oxidative stress response and sterigmatocystin production of <i>Aspergillus nidulans</i>. We found that NapA was important in the oxidative stress response by negatively regulating intracellular reactive species production and positively regulating catalase activities, whereas RsmA slightly negatively regulated catalase activities. Concerning sterigmatocystin production, the highest concentration was measured in the <i>ΔrsmA</i><i>ΔnapA</i> double deletion mutant, but elevated sterigmatocystin production was also found in the OE<i>rsmA</i> OE<i>napA</i> strain. Our results indicate that NapA influences sterigmatocystin production via regulating reactive species level whereas RsmA modulates toxin production independently of the redox regulation of the cells.
format	article
author	Bernadett Bákány Wen-Bing Yin Beatrix Dienes Tibor Nagy Éva Leiter Tamás Emri Nancy P. Keller István Pócsi
author_facet	Bernadett Bákány Wen-Bing Yin Beatrix Dienes Tibor Nagy Éva Leiter Tamás Emri Nancy P. Keller István Pócsi
author_sort	Bernadett Bákány
title	Study on the bZIP-Type Transcription Factors NapA and RsmA in the Regulation of Intracellular Reactive Species Levels and Sterigmatocystin Production of <i>Aspergillus nidulans</i>
title_short	Study on the bZIP-Type Transcription Factors NapA and RsmA in the Regulation of Intracellular Reactive Species Levels and Sterigmatocystin Production of <i>Aspergillus nidulans</i>
title_full	Study on the bZIP-Type Transcription Factors NapA and RsmA in the Regulation of Intracellular Reactive Species Levels and Sterigmatocystin Production of <i>Aspergillus nidulans</i>
title_fullStr	Study on the bZIP-Type Transcription Factors NapA and RsmA in the Regulation of Intracellular Reactive Species Levels and Sterigmatocystin Production of <i>Aspergillus nidulans</i>
title_full_unstemmed	Study on the bZIP-Type Transcription Factors NapA and RsmA in the Regulation of Intracellular Reactive Species Levels and Sterigmatocystin Production of <i>Aspergillus nidulans</i>
title_sort	study on the bzip-type transcription factors napa and rsma in the regulation of intracellular reactive species levels and sterigmatocystin production of <i>aspergillus nidulans</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/6d6a45e2103740de8f002ceb52796797
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Study on the bZIP-Type Transcription Factors NapA and RsmA in the Regulation of Intracellular Reactive Species Levels and Sterigmatocystin Production of <i>Aspergillus nidulans</i>

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